Scaffolds for dermal tissue engineering

Debels, Heidi; Wayne, Morrison

doi:10.1016/b978-0-08-102561-1.00006-3

Cited by 2 publications

(1 citation statement)

References 118 publications

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“…Bioengineered skin substitutes, like Apligraf or Dermagraft, utilize a skin substitute comprising living cells integrated into a matrix that provides structural support. The graft is positioned at the site of the chronic wound, facilitating the process of wound healing and recovery [22][23][24]. Another treatment, the sole biologically based treatment that is based on the activation of the AKT cascade, approved by the FDA, employs platelet-derived growth factor and is named Becaplarmin [25,26].…”

Section: Introductionmentioning

confidence: 99%

The Potential of PIP3 in Enhancing Wound Healing

Blitsman,

Hollander,

Benafsha

et al. 2024

IJMS

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Given the role of phosphatidylinositol 3,4,5-trisphosphate (PIP3) in modulating cellular processes such as proliferation, survival, and migration, we hypothesized its potential as a novel therapeutic agent for wound closure enhancement. In this study, PIP3 was examined in its free form or as a complex with cationic starch (Q-starch) as a carrier. The intracellular bioactivity and localization of free PIP3 and the Q-starch/PIP3 complexes were examined. Our results present the capability of Q-starch to form complexes with PIP3, facilitate its cellular membrane internalization, and activate intracellular paths leading to enhanced wound healing. Both free PIP3 and Q-starch/PIP3 complexes enhanced monolayer gap closure in scratch assays and induced amplified collagen production within HaCAT and BJ fibroblast cells. Western blot presented enhanced AKT activation by free or complexed PIP3 in BJ fibroblasts in which endogenous PIP3 production was pharmacologically inhibited. Furthermore, both free PIP3 and Q-starch/PIP3 complexes expedited wound closure in mice, after single or daily dermal injections into the wound margins. Free PIP3 and the Q-starch/PIP3 complexes inherently activated the AKT signaling pathway, which is responsible for crucial wound healing processes such as migration; this was also observed in wound assays in mice. PIP3 was identified as a promising molecule for enhancing wound healing, and its ability to circumvent PI3K inhibition suggests possible implications for chronic wound healing.

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Section: Introductionmentioning

confidence: 99%

The Potential of PIP3 in Enhancing Wound Healing

Blitsman,

Hollander,

Benafsha

et al. 2024

IJMS

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Ideal Living Skin Equivalents, From Old Technologies and Models to Advanced Ones: The Prospects for an Integrated Approach

Riabinin,

Pankratova,

Rogovaya

et al. 2024

BioMed Research International

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The development of technologies for the generation and transplantation of living skin equivalents (LSEs) is a significant area of translational medicine. Such functional equivalents can be used to model and study the morphogenesis of the skin and its derivatives, to test drugs, and to improve the healing of chronic wounds, burns, and other skin injuries. The evolution of LSEs over the past 50 years has demonstrated the leap in technology and quality and the shift from classical full‐thickness LSEs to principled new models, including modification of classical models and skin organoids with skin derived from human‐induced pluripotent stem cells (iPSCs) (hiPSCs). Modern methods and approaches make it possible to create LSEs that successfully mimic native skin, including derivatives such as hair follicles (HFs), sebaceous and sweat glands, blood vessels, melanocytes, and nerve cells. New technologies such as 3D and 4D bioprinting, microfluidic systems, and genetic modification enable achievement of new goals, cost reductions, and the scaled‐up production of LSEs.

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Scaffolds for dermal tissue engineering

Cited by 2 publications

References 118 publications

The Potential of PIP3 in Enhancing Wound Healing

The Potential of PIP3 in Enhancing Wound Healing

Ideal Living Skin Equivalents, From Old Technologies and Models to Advanced Ones: The Prospects for an Integrated Approach

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