Scaffolds the backbone of tissue engineering: Advancements in use of polyhydroxyalkanoates (PHA)

Dhania, Sunena; Bernela, Manju; Rani, Ruma; Parsad, Minakshi; Grewal, Sapna; Kumari, Santosh; Thakur, Rajesh

doi:10.1016/j.ijbiomac.2022.03.030

Cited by 32 publications

(14 citation statements)

References 170 publications

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“…Various strategies have been reported to overcome these barriers, including (i) usage of wild species bacteria that can readily produce PHBV without the addition of any precursors [ 80 , 81 , 82 ] and (ii) utilization of organic waste substrates for efficient production with low production costs [ 83 , 84 ]. Policastro et al (2021) have extensively reviewed various PHBV production strategies to enhance polymer productivity with reduced cost based on techno-economic analysis [ 85 ].…”

Section: Common Types Of Pha Used In Tissue Repair and Engineeringmentioning

confidence: 99%

Biomedical Applications of Polyhydroxyalkanoate in Tissue Engineering

et al. 2022

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Tissue engineering technology aids in the regeneration of new tissue to replace damaged or wounded tissue. Three-dimensional biodegradable and porous scaffolds are often utilized in this area to mimic the structure and function of the extracellular matrix. Scaffold material and design are significant areas of biomaterial research and the most favorable material for seeding of in vitro and in vivo cells. Polyhydroxyalkanoates (PHAs) are biopolyesters (thermoplastic) that are appropriate for this application due to their biodegradability, thermo-processability, enhanced biocompatibility, mechanical properties, non-toxicity, and environmental origin. Additionally, they offer enormous potential for modification through biological, chemical and physical alteration, including blending with various other materials. PHAs are produced by bacterial fermentation under nutrient-limiting circumstances and have been reported to offer new perspectives for devices in biological applications. The present review discusses PHAs in the applications of conventional medical devices, especially for soft tissue (sutures, wound dressings, cardiac patches and blood vessels) and hard tissue (bone and cartilage scaffolds) regeneration applications. The paper also addresses a recent advance highlighting the usage of PHAs in implantable devices, such as heart valves, stents, nerve guidance conduits and nanoparticles, including drug delivery. This review summarizes the in vivo and in vitro biodegradability of PHAs and conducts an overview of current scientific research and achievements in the development of PHAs in the biomedical sector. In the future, PHAs may replace synthetic plastics as the material of choice for medical researchers and practitioners.

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Section: Common Types Of Pha Used In Tissue Repair and Engineeringmentioning

confidence: 99%

Biomedical Applications of Polyhydroxyalkanoate in Tissue Engineering

et al. 2022

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“…The contact angle of the knitted structure, of which microscopic view is shown in Figure 3, was measured as 140º (standard deviation (sd): 4.68). Although the porous nature and mechanical properties of pile loop knit structures made up of PET yarns make them ideal candidates for cardiac tissue engineering their hydrophobic surface does not offer a favorable microenvironment for cell adhesion and proliferation [44]. Thus, scaffolds with hydrophobic nature can preferably be coated with ECM proteins (e.g., fibronectin, collagen type I) to promote cell attachment and migration [45].…”

Section: The Wettability Property Of Knit Structurementioning

confidence: 99%

In Vitro Evaluation of a Pile Loop Knitted Scaffold for Myocardial Tissue Engineering Using C2c12 Cells

HAROĞLU

2022

Tekstil Ve Mühendis

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Pile loop knitted scaffold has attracted attention for being used as a three dimensional (3D) biocompatible material particularly for the application of myocardial tissue engineering. In this research, nonintermingled, textured polyethylene terephthalate (PET) multifilament yarn with a filament count of 48 was used for producing a pile loop knit structure to investigate if structure could provide an appropriate microenvironment for the seeded murine C2C12 myoblasts in case of increasing in filament diameter (20.7 micron versus 9.1 micron from the previous study). The resulting structure had a pore network mainly consisting of macropores of 100-200 µm that were well interconnected with micropores (<90 µm). Furthermore, the mean values of the Young’s modulus of the structures at 20% strain were 240.67 kPa in the warp direction, and 85.73 kPa in the weft direction, which can be claimed not to be far from those declared in the literature for the human heart muscle. In addition, the cyclic loading-unloading test revealed no creep in the fabric after 100 cycles. According to the results of the WST-1 assay, the 48 filament-based scaffold was non-cytotoxic for C2C12 cells that were able to proliferate properly on the scaffold, which was visualized by SEM and confocal images. In this respect, pile loop knit structures keep promising results for being used as a cardiac patch.

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“…An emerging class of polymers suitable for a range of biomedical applications is the family of polyhydroxyalkanoates (PHAs), − which are aliphatic biodegradable polyesters synthesized in the form of intracellular granules by a wide range of microorganisms. PHAs have attracted increasing attention for tissue engineering applications due to their bio-origin and biodegradability, as well as their cytocompatibility and resorbability. , Depending on the biosynthetic production pathway, there is a wide variety of chemical PHA structures that vary in their monomeric composition or backbone chain-length, thereby offering a wide range of thermal and mechanical properties .…”

Section: Introductionmentioning

confidence: 99%

3D-Printed Poly(3-hydroxybutyrate-co-3-hydroxyhexanoate)-Cellulose-Based Scaffolds for Biomedical Applications

Giubilini,

Messori,

Bondioli

et al. 2023

Biomacromolecules

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While biomaterials have become indispensable for a wide range of tissue repair strategies, second removal procedures oftentimes needed in the case of non-bio-based and nonbioresorbable scaffolds are associated with significant drawbacks not only for the patient, including the risk of infection, impaired healing, or tissue damage, but also for the healthcare system in terms of cost and resources. New biopolymers are increasingly being investigated in the field of tissue regeneration, but their widespread use is still hampered by limitations regarding mechanical, biological, and functional performance when compared to traditional materials. Therefore, a common strategy to tune and broaden the final properties of biopolymers is through the effect of different reinforcing agents. This research work focused on the fabrication and characterization of a bio-based and bioresorbable composite material obtained by compounding a poly(3hydroxybutyrate-co-3-hydroxyhexanoate) (PHBH) matrix with acetylated cellulose nanocrystals (CNCs). The developed biocomposite was further processed to obtain three-dimensional scaffolds by additive manufacturing (AM). The 3D printability of the PHBH−CNC biocomposites was demonstrated by realizing different scaffold geometries, and the results of in vitro cell viability studies provided a clear indication of the cytocompatibility of the biocomposites. Moreover, the CNC content proved to be an important parameter in tuning the different functional properties of the scaffolds. It was demonstrated that the water affinity, surface roughness, and in vitro degradability rate of biocomposites increase with increasing CNC content. Therefore, this tailoring effect of CNC can expand the potential field of use of the PHBH biopolymer, making it an attractive candidate for a variety of tissue engineering applications.

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Scaffolds the backbone of tissue engineering: Advancements in use of polyhydroxyalkanoates (PHA)

Cited by 32 publications

References 170 publications

Biomedical Applications of Polyhydroxyalkanoate in Tissue Engineering

Biomedical Applications of Polyhydroxyalkanoate in Tissue Engineering

In Vitro Evaluation of a Pile Loop Knitted Scaffold for Myocardial Tissue Engineering Using C2c12 Cells

3D-Printed Poly(3-hydroxybutyrate-co-3-hydroxyhexanoate)-Cellulose-Based Scaffolds for Biomedical Applications

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