Scaffolds with high oxygen content support osteogenic cell survival under hypoxia

Augustine, Robin; Camci‐Unal, Gulden

doi:10.1039/d3bm00650f

Cited by 7 publications

(3 citation statements)

References 42 publications

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“…However, an increase in CPN content in the PCL beyond 3% w/w led to a reduction in tensile strength, elongation at break, and Young's modulus. The decrease was particularly noticeable in PCL-CPN-6 scaffolds, as reported previously [29]. This finding can be a result the Payne effect, a non-linear relationship between stress and strain in reinforced polymer systems.…”

Section: Mechanical Properties Of the Composite Self-oxygenating Scaf...supporting

confidence: 86%

Hydrogel-Impregnated Self-Oxygenating Electrospun Scaffolds for Bone Tissue Engineering

2023

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Bone defects resulting from trauma, disease, or aging present significant challenges in the clinic. Although biomaterial scaffolds for bone-tissue engineering have shown promising results, challenges remain, including the need for adequate mechanical strength and suitable bioactive agents within scaffolds to promote bone formation. Oxygen is a critical factor for successful bone formation, and low oxygen tension inhibits it. In this study, we developed gelatin methacryloyl (GelMA) hydrogel-impregnated electrospun polycaprolactone (PCL) scaffolds that can release oxygen over 3 weeks. We investigated the potential of composite scaffolds for cell survival in bone-tissue engineering. Our results showed that the addition of an increased amount of CaO2 nanoparticles to the PCL scaffolds significantly increased oxygen generation, which was modulated by GelMA impregnation. Moreover, the resulting scaffolds showed improved cytocompatibility, pre-osteoblast adhesion, and proliferation under hypoxic conditions. This finding is particularly relevant since hypoxia is a prevalent feature in various bone diseases. In addition to providing oxygen, CaO2 nanoparticles also act as reinforcing agents improving the mechanical property of the scaffolds, while the incorporation of GelMA enhances cell adhesion and proliferation properties. Overall, our newly developed self-oxygenating composite biomaterials are promising scaffolds for bone-tissue engineering applications.

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Section: Mechanical Properties Of the Composite Self-oxygenating Scaf...supporting

confidence: 86%

Hydrogel-Impregnated Self-Oxygenating Electrospun Scaffolds for Bone Tissue Engineering

2023

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“…In the field of bone tissue engineering, the optimal scaffolds are intended to biomimic the mechanical and biological characteristics of various bone types . Moreover, proliferating migration, multiplication, and transformation of cells are influenced by the physical attributes of scaffolds . Researchers have been studying the connection between the cellular biofunctioning of various cells and the mechanical characteristics of scaffolds over the past few years.…”

Section: Ideal Scaffold For Bone Tissue Engineeringmentioning

confidence: 99%

“…118 Moreover, proliferating migration, multiplication, and transformation of cells are influenced by the physical attributes of scaffolds. 119 Researchers have been studying the connection between the cellular biofunctioning of various cells and the mechanical characteristics of scaffolds over the past few years. Scaffolds' chemical ingredients, compositions, and production techniques have all been changed to modify their mechanical and physical characteristics.…”

Section: Ideal Scaffold For Bone Tissue Engineeringmentioning

confidence: 99%

Current Perspectives of Protein in Bone Tissue Engineering: Bone Structure, Ideal Scaffolds, Fabrication Techniques, Applications, Scopes, and Future Advances

Aslam Khan,

Aslam,

Bin Abdullah

et al. 2024

ACS Appl. Bio Mater.

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In view of their exceptional approach, excellent inherent biocompatibility and biodegradability properties, and interaction with the local extracellular matrix, protein-based polymers have received attention in bone tissue engineering, which is a multidisciplinary field that repairs and regenerates fractured bones. Bone is a multihierarchical complex structure, and it performs several essential biofunctions, including maintaining mineral balance and structural support and protecting soft organs. Protein-based polymers have gained interest in developing ideal scaffolds as emerging biomaterials for bone fractured healing and regeneration, and it is challenging to design ideal bone substitutes as perfect biomaterials. Several protein-based polymers, including collagen, keratin, gelatin, serum albumin, etc., are potential materials due to their inherent cytocompatibility, controlled biodegradability, high biofunctionalization, and tunable mechanical characteristics. While numerous studies have indicated the encouraging possibilities of proteins in BTE, there are still major challenges concerning their biodegradability, stability in physiological conditions, and continuous release of growth factors and bioactive molecules. Robust scaffolds derived from proteins can be used to replace broken or diseased bone with a biocompatible substitute; proteins, being biopolymers, provide excellent scaffolds for bone tissue engineering. Herein, recent developments in protein polymers for cutting-edge bone tissue engineering are addressed in this review within 3−5 years, with a focus on the significant challenges and future perspectives. The first section discusses the structural fundamentals of bone anatomy and ideal scaffolds, and the second section describes the fabrication techniques of scaffolds. The third section highlights the importance of proteins and their applications in BTE. Hence, the recent development of protein polymers for state-of-the-art bone tissue engineering has been discussed, highlighting the significant challenges and future perspectives.

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Microwave‐Responsive Nanoplatform with Robust ROS Generation and Inhibiting Effects of Two‐Component System and Quorum Sensing for the Treatment of MRSA‐Induced Osteomyelitis

Cheng,

Yang,

Wang

et al. 2024

Adv Funct Materials

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The hypoxic environment of deep tissue and the weak energy of microwave (MW) are still the major limitations of MW catalytic therapy. In this work, an MW‐responsive nano‐platform consisting of an Mn‐doped porphyrin metal–organic framework loaded with calcium peroxide (Mn0.1PCC) is designed to regulate the microenvironment of the injection site and enhance the generation of reactive oxygen species. The excellent MW absorbing properties of Mn0.1PCC improve the Fenton reaction between hydrogen peroxide formed by the hydrolysis of calcium peroxide and released Mn2+ generating more hydroxyl radicals under MW. This process does not consume the oxygen substrate and even produces oxygen to improve the hypoxic environment. Therefore, at a low and safe concentration (125 µg mL−1), the antibacterial rate of Mn0.1PCC against 4 × 108 CFU mL−1 of methicillin‐resistant Staphylococcus aureus (MRSA) after MW irradiation for 15 min is 99.71% ± 0.18%. Mn0.1PCC, upon exposure to MW, prevents the recurrence of bacterial infections and overcomes drug resistance by inhibiting quorum sensing and the two‐component system of MRSA. It can also induce macrophage M1 polarization, up‐regulate the pro‐inflammatory factor tumor necrosis factor‐α, and enhance the phagocytosis of macrophage to bacteria. This strategy provides insights into the development of high‐performance MW‐responsive nanocomposites for the treatment of infections in deep tissues.

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Scaffolds with high oxygen content support osteogenic cell survival under hypoxia

Abstract: Novel oxygen-generating scaffold with a controlled oxygen release profile can support osteogenic cells under hypoxia and is a promising solution for bone tissue engineering.

Cited by 7 publications

References 42 publications

Hydrogel-Impregnated Self-Oxygenating Electrospun Scaffolds for Bone Tissue Engineering

Hydrogel-Impregnated Self-Oxygenating Electrospun Scaffolds for Bone Tissue Engineering

Current Perspectives of Protein in Bone Tissue Engineering: Bone Structure, Ideal Scaffolds, Fabrication Techniques, Applications, Scopes, and Future Advances

Microwave‐Responsive Nanoplatform with Robust ROS Generation and Inhibiting Effects of Two‐Component System and Quorum Sensing for the Treatment of MRSA‐Induced Osteomyelitis

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