Scalable Milk-Derived Whey Protein Hydrogel as an Implantable Biomaterial

Hu, Ziyi; Cao, Wangbei; Shen, Lishui; Sun, Ziyang; Kang, Yu Min; Zhu, Qinchao; Ren, Tanchen; Zhang, Liwen; Zheng, Houwei; Gao, Changyou; He, Yong; Guo, Chengchen; Zhu, Yang; Ren, Dawei

doi:10.1021/acsami.2c02361

Cited by 14 publications

(11 citation statements)

References 40 publications

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“…Alginate was methacrylated and previously characterized via 1 H NMR using our published methods. ,, Applying those same methodologies, WPI was also methacrylated to enable covalent bonding with AlgMA, forming a cross-linked network. , Alginate was methacrylated and previously characterized via 1 H NMR using our published methods. ,, Applying those same methodologies, WPI was also methacrylated to enable covalent bonding with AlgMA, forming a cross-linked network Figure A) spectral results verified the introduction of methyl protons on the protein backbone at 5.4 and 5.7 ppm and the disappearance of the peak at 4.6 ppm and supported by spectral differences in peaks between 2 and 1 ppm and differences in the WPI-MA material compared to the nonmodified material. , The AlgMA and WPI-MA systems enabled the formation of a hydrogel with varying physical and mechanical properties. The whey protein and AlgMA hydrogels were used as a model system since we can control the mechanical properties and are familiar with the cross-linking system due to previous studies; however, future work will focus on different chemistry and food-safe hydrogel formation methods.…”

Section: Resultsmentioning

confidence: 65%

Whey Protein Isolate Composites as Potential Scaffolds for Cultivated Meat

Charron,

Tahir,

Foley

et al. 2024

ACS Appl. Bio Mater.

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Modern food technology has given rise to numerous alternative protein sources in response to a growing human population and the negative environmental impacts of current food systems. To aid in achieving global food security, one such form of alternative protein being investigated is cultivated meat, which applies the principles of mechanical and tissue engineering to produce animal proteins and meat products from animal cells. Herein, nonmodified and methacrylated whey protein formed hydrogels with methacrylated alginate as potential tissue engineering scaffolds for cultivated meat. Whey protein is a byproduct of dairy processing and was selected because it is an approved food additive and cytocompatible and has shown efficacy in other biomaterial applications. Whey protein and alginate scaffolds were formed via visible light cross-linking in aqueous solutions under ambient conditions. The characteristics of the precursor solution and the physical−mechanical properties of the scaffolds were quantified; while gelation occurred within the homo-and copolymer hydrogels, the integrity of the network was significantly altered with varying components. Qualitatively, the scaffolds exhibited a three-dimensional (3D) interconnected porous network. Whey protein isolate (WPI)-based scaffolds were noncytotoxic and supported in vitro myoblast adhesion and proliferation. The data presented support the hypothesis that the composition of the hydrogel plays a significant role in the scaffold's performance.

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Section: Resultsmentioning

confidence: 65%

Whey Protein Isolate Composites as Potential Scaffolds for Cultivated Meat

Charron,

Tahir,

Foley

et al. 2024

ACS Appl. Bio Mater.

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“…Thus, it is feasible to predict how α-LAMA hydrogels will degrade in the future, with broader applications. Matrix metallopeptidase-9 (MMP-9) cleavage sites were discovered in α-LA, supporting the subcutaneous breakdown of bulk hydrogels in mice. The α-LAMA hydrogel is flexible during processing, enabling it to take up various forms, including particles and bespoke scaffolds, and to allow for various other implantation techniques.…”

Section: Resultsmentioning

confidence: 99%

Rapid UV Photo-Cross-Linking of α-Lactalbumin Hydrogel Biomaterial To Enable Wound Healing

Huang,

Zhu,

Zhu

et al. 2023

ACS Omega

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Although both the function and biocompatibility of protein-based biomaterials are better than those of synthetic materials, their usage as medical material is currently limited by their high costs, low yield, and low batch-to-batch reproducibility. In this article, we show how α-lactalbumin (α-LA), rich in tryptophan, was used to produce a novel type of naturally occurring, protein-based biomaterial suitable for wound dressing. To create a photo-cross-linkable polymer, α-LA was methacrylated at a 100-g batch scale with >95% conversion and 90% yield. α-LAMA was further processed using photo-cross-linking-based advanced processing techniques such as microfluidics and 3D printing to create injectable hydrogels, monodispersed microspheres, and patterned scaffolds. The obtained α-LAMA hydrogels show promising biocompatibility and degradability during in vivo testing. Additionally, the α-LAMA hydrogel can accelerate post-traumatic wound healing and promote new tissue regeneration. In conclusion, cheap and safe α-LAMA-based biomaterials could be produced, and they have a beneficial effect on wound healing. As a result, there may arise a potential partnership between the dairy industry and the development of pharmaceuticals.

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“…3D printing has been carried out as in our previous studies [ 29 ]. DLP photopatterning experiments were conducted using a high-quality DLP printer for laboratory use.…”

Section: Methodsmentioning

confidence: 99%

White-light crosslinkable milk protein bioadhesive with ultrafast gelation for first-aid wound treatment

et al. 2023

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Background Post-traumatic massive hemorrhage demands immediately available first-aid supplies with reduced operation time and good surgical compliance. In-situ crosslinking gels that are flexibly adapting to the wound shape have a promising potential, but it is still hard to achieve fast gelation, on-demand adhesion, and wide feasibility at the same time. Methods A white-light crosslinkable natural milk-derived casein hydrogel bioadhesive is presented for the first time. Benefiting from abundant tyrosine residues, casein hydrogel bioadhesive was synthesized by forming di-tyrosine bonds under white light with a ruthenium-based catalyst. We firstly optimized the concentration of proteins and initiators to achieve faster gelation and higher mechanical strength. Then, we examined the degradation, cytotoxicity, tissue adhesion, hemostasis, and wound healing ability of the casein hydrogels to study their potential to be used as bioadhesives. Result Rapid gelation of casein hydrogel is initiated with an outdoor flashlight, a cellphone flashlight, or an endoscopy lamp, which facilitates its usage during first-aid and minimally invasive operations. The rapid gelation enables 3D printing of the casein hydrogel and excellent hemostasis even during liver hemorrhage due to section injury. The covalent binding between casein and tissue enables robust adhesion which can withstand more than 180 mmHg blood pressure. Moreover, the casein-based hydrogel can facilitate post-traumatic wound healing caused by trauma due to its biocompatibility. Conclusion Casein-based bioadhesives developed in this study pave a way for broad and practical application in emergency wound management.

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Scalable Milk-Derived Whey Protein Hydrogel as an Implantable Biomaterial

Cited by 14 publications

References 40 publications

Whey Protein Isolate Composites as Potential Scaffolds for Cultivated Meat

Whey Protein Isolate Composites as Potential Scaffolds for Cultivated Meat

Rapid UV Photo-Cross-Linking of α-Lactalbumin Hydrogel Biomaterial To Enable Wound Healing

White-light crosslinkable milk protein bioadhesive with ultrafast gelation for first-aid wound treatment

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