Scalable ultrasmall three-dimensional nanowire transistor probes for intracellular recording

Zhao, Yunlong; You, Siheng Sean; Zhang, Anqi; Lee, Jae Hyun; Huang, Jinlin; Lieber, Charles M.

doi:10.1038/s41565-019-0478-y

Cited by 146 publications

(151 citation statements)

References 41 publications

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“…Indeed, increased study of the cascade of areas within the three-dimensional distribution of circuits in the brain appears to be the future of neuroscience. 15,[23][24][25] The extremely high shank density we achieved (exceeding 6000 sites/cm 2 ) represents an order of magnitude improvement over current microelectrode techniques. Second, as shown in figures 1 and 4, the metal-polymer printing developed in this paper allows rapid prototyping and custom routing of signals from shanks via changes to the printing programs.…”

Section: Discussionmentioning

confidence: 85%

CMU Array: A 3D Nano-Printed, Customizable Ultra-High-Density Microelectrode Array Platform

Saleh

Ritchie

Nicholas

et al. 2019

Preprint

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Microelectrode arrays (MEAs) provide the means to record electrophysiological activity fundamental to both basic and clinical neuroscience (e.g. brain-computer interfaces). Despite recent advances, current MEAs have significant limitations -including recording density, fragility, expense, and the inability to optimize the probe to individualized study or patient needs.Here we address the technological limitations through the utilization of the newest developments in 3D nanoparticle printing. 1 Our 'CMU Arrays' possess previously impossible electrode densities (> 6000 channels/cm 2 ) with tip diameters as small as 10µm. Most importantly, the probes are entirely customizable owing to the adaptive manufacturing process. Any combination of individual shank lengths, impedances, and layouts are possible. This is achieved in part via our new multi-layer, multi material, custom 3D-printed circuit boards, a fabrication advancement in itself. This device design enables new experimental avenues of targeted, large-scale recording of electrical signals from a variety of biological tissues.

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Section: Discussionmentioning

confidence: 85%

CMU Array: A 3D Nano-Printed, Customizable Ultra-High-Density Microelectrode Array Platform

Saleh

Ritchie

Nicholas

et al. 2019

Preprint

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“…Although most of the demonstrated nanoprobes used by the Lieber group are single‐probe platforms, the success of using phospholipids presents a promising strategy to facilitate cell membrane penetration by nanowire‐based probes. Very recently, Zhao et al exploited nanoscale membrane curvature when designing U‐shaped nanowire field‐effect transistor probe arrays, [ 161 ] which facilitated the internalization of these phospholipid‐coated probes with cell membranes (Figure 9d,e). This scalable probe array not only possessed the capability for high‐amplitude intracellular recordings, but also achieved parallel intracellular recording within individual cells and cell networks.…”

Section: Assisted Penetrationmentioning

confidence: 99%

Nanoneedle Platforms: The Many Ways to Pierce the Cell Membrane

He¹,

Hu²,

et al. 2020

Adv Funct Materials

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Establishing techniques to efficiently and nondestructively access the intracellular milieu is essential for many biomedical and scientific applications, ranging from drug delivery, to electrical recording, to biochemical detection. Cell penetration using nanoneedle arrays is currently a research focus area because it not only meets the increasing therapeutic demands of cell modifications and genome editing, but also provides an ideal platform for tracking long-term intracellular information. Although the precise mechanism driving membrane penetration by nanoneedle arrays is still unclear, the low cytotoxicity, wide range of delivered materials, diverse cell type targets, and simple material structures of nanoneedle arrays make these splendid platforms for cell access. Here, the recent progress in this field is reviewed by examining device architectures and discussing mechanisms for nanoneedle penetration, and the major studies demonstrating the most general applicability of nanoneedle arrays, typical methodologies to access the intracellular environment using nanoneedles with spontaneous or assisted penetration modes, as well as biosafety aspects are presented. This review should be valuable for deeply understanding the materials fabrication principles, device designs, cell penetration methodologies, biosafety aspects, and application strategies of nanoneedle array-based systems that are of crucial importance for the development of future practical biomedical platforms.

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“…F, Simultaneous intracellular recording from one DRG neuron by two ~50‐nm FETs with a 0.75‐μm ROC on one probe arm with a 2‐μm separation (i); derivative of traces in the region marked by the dashed box (ii). Reproduced with permission . Copyright 2014, Nature Publishing Group.…”

Section: Chemical/biological Sensorsmentioning

confidence: 99%

“…This approach exhibits simplicity, single viral particle sensitivity, and capability for selective multiplexed detection in solution with application in biological electronics. 186 Copyright 2014, Nature Publishing Group. FET, field-effect transistor; ROC, radii of curvature One amazing application of nanowires is the realization of free-standing and moveable sensor probes that can be integrated with specific functionalities.…”

Section: Nanowire Biological Sensorsmentioning

confidence: 99%

“…Recently, these authors further proposed scalable ultrasmall 3D nanowire transistor probes for intracellular recording. 186 Integrated applications of advanced micro-and nanofabrication technologies, such as ALD, photolithography, and electron beam lithography, were employed to fabricate U-shaped nanowire FETs ( Figure 17A). The resulting device displayed the capability to record full amplitude intracellular action potentials similar to patch-clamp recordings.…”

Section: Nanowire Biological Sensorsmentioning

confidence: 99%

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Compositional and structural engineering of inorganic nanowires toward advanced properties and applications

Sun

et al. 2019

InfoMat

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As natural one‐dimensional confined electron transport channels and photon propagation paths, nanowires (NWs) display unmatched properties and huge potential for application in diverse fields. The ability to construct a nanoscale functional system would enable significant advances in the currently desired miniaturized device integration. To date, the basic properties of all types of nanowire crystals, including metallic NWs, as well as group IV‐related, III‐V/II‐VI compounds, and metal oxide NWs, are well understood. Regarding future work, compositional (ie, doping and alloying) and structural (defect and heterostructure) designs to flexibly realize custom‐made functionality are emphasized. Along this line, recent progress is reviewed, including the basic properties (nanowire mechanics, electronics, and photonics) and applications such as photodetectors and chemical/biological sensors. A review of the correlations between the compositional/structural configuration of nanowires and the corresponding functionality is also presented. The future development direction of this field is concluded and highlighted at the end.

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Scalable ultrasmall three-dimensional nanowire transistor probes for intracellular recording

Cited by 146 publications

References 41 publications

CMU Array: A 3D Nano-Printed, Customizable Ultra-High-Density Microelectrode Array Platform

CMU Array: A 3D Nano-Printed, Customizable Ultra-High-Density Microelectrode Array Platform

Nanoneedle Platforms: The Many Ways to Pierce the Cell Membrane

Compositional and structural engineering of inorganic nanowires toward advanced properties and applications

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