Scale‐Up and Scale‐Down

Noorman, Henk

doi:10.1002/9783527697441.ch16

Cited by 3 publications

(5 citation statements)

References 59 publications

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“…In many cases, the low productivity of secondary metabolites by plant suspension cells requires a scale-up in bioreactor technology of tens of cubic meters in order to achieve an economical production process. In general, a correlation of fixed costs per kg product to bioreactor volume to the power of minus two thirds is assumed, meaning products become about five times cheaper with a ten-fold increase in production volume [75]. Currently, the scale-up of agitated vessels for plant suspension cell cultures generally follows particular rules that date back several decades and are mostly based on a certain level of geometric similarity between the vessels [76].…”

Section: Engineering and Scale-up Considerations 41 Plant Cell Suspension Rheologymentioning

confidence: 99%

Bioreactor Technology for Sustainable Production of Plant Cell-Derived Products

Werner

Maschke

Eibl

et al. 2018

Reference Series in Phytochemistry

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Section: Engineering and Scale-up Considerations 41 Plant Cell Suspension Rheologymentioning

confidence: 99%

Bioreactor Technology for Sustainable Production of Plant Cell-Derived Products

Werner

Maschke

Eibl

et al. 2018

Reference Series in Phytochemistry

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“…are well‐known for their high tolerance toward toxic chemicals including solvents (Nikel, Martinez‐Garcia, & de Lorenzo, ; Wierckx, Ballerstedt, de Bont, & Wery, ), the robustness of metabolism (Belda et al, ), and flexibility in adaptation to various environmental fluctuations and stimuli, for example, oxidative stress (Ebert, Kurth, Grund, Blank, & Schmid, ). However, P. putida is an obligate aerobic bacteria, which in industrial scale production will often encounter the problems of oxygen transfer, oxidative carbon loss, and more investment in expensive equipment and additional utility (Noorman, ). On the other hand, a classical anaerobic bacterium will typically have many alternative pathways to ferment the provided substrate into undesirable by‐products.…”

Section: Introductionmentioning

confidence: 99%

Improved performance of Pseudomonas putida in a bioelectrochemical system through overexpression of periplasmic glucose dehydrogenase

Lai

Plan

et al. 2017

Biotech & Bioengineering

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It was recently demonstrated that a bioelectrochemical system (BES) with a redox mediator allowed Pseudomonas putida to perform anoxic metabolism, converting sugar to sugar acids with high yield. However, the low productivity currently limits the application of this technology. To improve productivity, the strain was optimized through improved expression of glucose dehydrogenase (GCD) and gluconate dehydrogenase (GAD). In addition, quantitative real-time RT-PCR analysis revealed the intrinsic self-regulation of GCD and GAD. Utilizing this self-regulation system, the single overexpression strain (GCD) gave an outstanding performance in the electron transfer rate and 2-ketogluconic acid (2KGA) productivity. The peak anodic current density, specific glucose uptake rate and 2KGA producing rate were 0.12 mA/cm 2 , 0.27 ± 0.02 mmol/g CDW /hr and 0.25 ± 0.02 mmol/ g CDW /hr, which were 327%, 477%, and 644% of the values of wild-type P. putida KT2440, respectively. This work demonstrates that expression of periplasmic dehydrogenases involved in electron transfer can significantly improve productivity in the BES.anode respiration, bioelectrochemical system, membrane-bound dehydrogenase, microbial electrosynthesis, Pseudomonas putida

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“…Interestingly, the scale-dependence of gas–liquid mass transfer is different from liquid–liquid mass transfer (see Table ). Mixing is a function of power input, which can vary significantly with scale . More specifically, it is easier to thoroughly mix a smaller volume to achieve good dispersion (high power input per unit volume) than it is to mix a large volume.…”

Section: Introductionmentioning

confidence: 99%

“… a The extent of effect of hold-up on mass transfer is more or less pronounced for plug-flow or well-mixed regimes, respectively b Stable emulsions create a homogeneous medium that will be independent of mixing. …”

Section: Introductionmentioning

confidence: 99%

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Scoping the Enantioselective Desymmetrization of a Poorly Water-Soluble Diester by Recombinant Pig Liver Esterase

Meissner

Süss²,

Brundiek³

et al. 2018

Org. Process Res. Dev.

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Previously, the biocatalytic desymmetrization of dimethyl cyclohex-4-ene-cis-1,2dicarboxylate to (1S,2R)-1-(methoxycarbonyl)cyclohex-4-ene-2-carboxylic acid, an important intermediate towards the synthesis of biologically active molecules, had been well-characterized in terms of pH and temperature optima and several aspects of process performance. Eventually this promising reaction could convert 200 mM (40 g·L -1 ) of substrate with > 99.5% e.e. using the recombinant pig liver esterase, ECS-PLE06, at a scale of 8.8 L. However, the precise influence of substrate concentration and the poorly water-soluble nature of the substrate (approximately 60 mM in water at 25 °C for structurally similar dimethyl 1,4-cyclohexanedicarboxylate) remained elusive. Therefore, this work focuses on using a recently published methodology based on reaction trajectory analysis to identify mass transfer limitations in this reaction. With the constraints of mass transfer on space-time yield considered, it was possible to evaluate and improve biocatalyst yield (mass of product per mass of biocatalyst) through the use of higher substrate concentrations.Ultimately the complete conversion of approximately 75 g·L -1 substrate was achieved in 3.65 h yielding an excellent productivity of 20 g·L -1 ·h -1 with a biocatalyst yield of 4.36 g·g biocat -1 . This work also highlights the simplicity of applying a reaction trajectory analysis methodology, importance of scale during reaction characterizations and identifies future directions for reaction improvement to address substrate supply and product inhibition/deactivation.

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Scale‐Up and Scale‐Down

Cited by 3 publications

References 59 publications

Bioreactor Technology for Sustainable Production of Plant Cell-Derived Products

Bioreactor Technology for Sustainable Production of Plant Cell-Derived Products

Improved performance of Pseudomonas putida in a bioelectrochemical system through overexpression of periplasmic glucose dehydrogenase

Scoping the Enantioselective Desymmetrization of a Poorly Water-Soluble Diester by Recombinant Pig Liver Esterase

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