Scale up of production in a bioreactor of a halotolerant protease from moderately halophilic Bacillus sp. isolated from soil

Prasad, Roopa; Abraham, Theruvath Koshy; Nair, A. Jayakumaran

doi:10.1590/s1516-89132014005000014

Cited by 15 publications

(9 citation statements)

References 25 publications

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“…The bacterium was isolated from poultry soil and was identified as Bacillus sp. MTCC 9558 belonging to B. cereus cluster was found to be a good producer of protease reported by us earlier (Prasad et al 2014).…”

Section: Microorganismsupporting

confidence: 58%

Production and Characterization of Bioactive Peptides by Purified Protease Isolated From Bacillus Sp. Mtcc 9558

Prasad¹,

Nair

2021

CTBP

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Section: Microorganismsupporting

confidence: 58%

Production and Characterization of Bioactive Peptides by Purified Protease Isolated From Bacillus Sp. Mtcc 9558

Prasad¹,

Nair

2021

CTBP

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“…The study of NA7 growth kinetics with reference to protease production demonstrated that, prior to the end of the exponential phase, the culture showed marked protease production efficiency with relatively stable maximum activity records (0.610-0.625 TU) for more than 60 h during the stationary phase. When compared to other similar halotolerant Bacillus shake flask condition (Prasad et al, 2014), the expression phase of the proteolytic activity is very much longer.…”

Section: Discussionmentioning

confidence: 88%

Isolation and characterization of a haloalkaliphilic protease producer bacterium from Wadi Natrun in Egypt

Lotfy¹,

Abd-El-Karim²,

El-Sharouny³

et al. 2017

Afr. J. Biotechnol.

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A Gram-positive, rod-shaped, spore-forming haloalkaliphilic bacterium designated as NA7 was isolated from the surface of a Helianthemum nummularium root sample obtained from Wadi Natrun in Egypt. Sequence analysis of the 16S rRNA gene revealed a Bacillus haloalkaliphilius strain as the closest match with 99% identity. In a shake flask culture containing 10% NaCl, adjusted to pH 10 and incubated at 37°C, the isolated strain produced thermostable extracellular alkaline protease with relatively stable maximum activity records (0.610-0.625 TU) within a relatively long stationary phase that exceeded 60 h. A 2-level fractional factorial design (Plackett-Burman) was then applied to screen for nutritional and cultivation factors regulating protease production by the isolate and to appraise their effects. Calculated statistical parameters revealed that NaCl and MgSO 4 are the most significant independent variables affecting alkaline protease production by NA7 and suggested a near-optimum culture condition. Verification of this predicted condition resulted in an alkaline protease specific activity record of 509 TU/mg protein with a 1.27 fold increase when compared to the basal medium culture.

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“…The refractive index obtained is 1.3381 so the concentration of ethanol was 8 % w/w. Agro-based byproducts as being used as an alternate substrate for the commercial production of several industrially important products [9]. Based on the type of feedstock used, industrial bioethanol production is divided into three generations and approximate ethanol yields from different types of feedstocks have been studied extensively [10].…”

Section: Concentration Of Ethanolmentioning

confidence: 99%

Design of Experimental Lab Scale Vertical Mass Flow Type Bioreactor for Bioethanol Production by Co-Culture Strategy from Cassava Waste

Prasad¹,

Jijina²,

Akhila³

et al. 2023

J. Sci. Res.

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Due to a surge in demand and cost, there is a requirement for alternative energy sources by devising strategies for the efficient production of biofuels. Immobilized microbial systems for the conversion of biomass to fuels have become progressively important. Cassava (Manihot esculentum) processing waste, a massive byproduct of starch processing is utilized in this work for bioethanol production. This study was an attempt to design and develop an experimental lab-scale Vertical Mass-Flow type Bioreactor (VMFB) demarcated into aerobic and anaerobic zones to produce bioethanol. The upper aerobic zone was meant for saccharification and the lower anaerobic zone for fermentation, the technique is called Simultaneous Saccharification and Fermentation (SSF). The feasibility of co-immobilizing saccharification strains (A. awamori and D. bruxellensis) and fermentation strains (Z. mobilis) for bioethanol production through SSF from cassava agro-waste were tested. Polyurethane foam was used in the aerobic reaction zone and calcium-alginate beads immobilized microorganisms in the anaerobic reaction zone were employed as carriers for the immobilization. The main objective of this study was to understand the usability of agricultural waste, especially cassava processing waste as raw material for bioethanol production, using SSF technology a concentration of 8 % w/w ethanol was obtained.

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Scale up of production in a bioreactor of a halotolerant protease from moderately halophilic Bacillus sp. isolated from soil

Abstract: Studies were conducted on the production of protease by moderately halophilic

Cited by 15 publications

References 25 publications

Production and Characterization of Bioactive Peptides by Purified Protease Isolated From Bacillus Sp. Mtcc 9558

Production and Characterization of Bioactive Peptides by Purified Protease Isolated From Bacillus Sp. Mtcc 9558

Isolation and characterization of a haloalkaliphilic protease producer bacterium from Wadi Natrun in Egypt

Design of Experimental Lab Scale Vertical Mass Flow Type Bioreactor for Bioethanol Production by Co-Culture Strategy from Cassava Waste

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