Scale up production of isoflavonoids in cell suspension cultures of <i>Pueraria tuberosa</i> grown in shake flasks and bioreactor

Sharma, Varsha; Goyal, Shaily; Ramawat, K. G.

doi:10.1002/elsc.200800114

Cited by 24 publications

(10 citation statements)

References 17 publications

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“…Studies dealing with biotechnological production of isoflavones are mainly based on suspension cultures of Pueraria sp. (Li and Zhang 2006; Goyal and Ramawat 2008a, b; Sharma et al 2009), Psoralea sp. (Shinde et al 2009a), Glycine max (Federici et al 2003; Terrier et al 2007) and Genista tinctoria (Luczkiewicz and Glod 2005; Luczkiewicz and Kokotkiewicz 2012).…”

Section: Introductionmentioning

confidence: 99%

Isoflavone production in Cyclopia subternata Vogel (honeybush) suspension cultures grown in shake flasks and stirred-tank bioreactor

Kokotkiewicz

Łuczkiewicz

Kowalski

et al. 2013

Appl Microbiol Biotechnol

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Suspension cultures of the endemic South-African plant Cyclopia subternata were established for the first time and evaluated for the presence of isoflavones. The influence of light, as well as medium supplementation strategies with phenylalanine, casein hydrolysate and coconut water on biomass growth and isoflavone production were examined. The highest levels of 7-O-β-glucosides of calycosin, pseudobaptigenin and formononetin (275.57, 125.37 and 147.28 mg/100 g DW, respectively) were recorded for cultures grown in the absence of light, whereas coconut water substantially promoted biomass growth. Cell suspensions were subsequently grown in the 2-l stirred-tank bioreactor. Maximum productivity of 7-O-β-glucosides of calycosin, pseudobaptigenin and formononetin (0.96, 0.44 and 0.22 mg l−1 day−1, respectively) in bioreactor-cultivated cells was obtained for biomass grown in the dark and supplemented with coconut water. The results indicate that C. subternata suspension cultures can be utilised for the production of the specified isoflavone derivatives absent in the intact plant.

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Section: Introductionmentioning

confidence: 99%

Isoflavone production in Cyclopia subternata Vogel (honeybush) suspension cultures grown in shake flasks and stirred-tank bioreactor

Kokotkiewicz

Łuczkiewicz

Kowalski

et al. 2013

Appl Microbiol Biotechnol

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“…Biotechno-logical production of isofl avones, mainly from the family Fabaceae, is based on suspension cultures of Pueraria sp. (Goyal and Ramawat 2008a , b ;Sharma et al 2009 ;He et al 2011 ), Psoralea sp. (Shinde et al 2009a , b ), and Glycine max (Federici et al 2003 ;Terrier et al 2007 ).…”

Section: Isofl Avone Productionmentioning

confidence: 99%

“…An increased secondary metabolite production is correlated with a slow cell division rate in cell suspension cultures (Lindsey and Yeoman 1983 ;Sharma et al 2011 ). Similarly, secondary metabolite production at the stationary phase of growth has been related with tissue organization (Tabata et al 1972 ), morphological differentiation (Ramawat et al 1985 ;Sharma et al 2009 ), and low growth rates (Lindsey and Yeoman 1983 ).…”

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confidence: 97%

Improving Secondary Metabolite Production in Tissue Cultures

Kumar

2015

Plant Biology and Biotechnology

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Plant cell and tissue culture has been suggested as an alternative means for year-round production of secondary metabolites with an added potential of increasing yields by culture selection and manipulation, genetic transformation, hairy root cultures, and use of bioreactors for mass production. Secondary metabolite pathways and genes involved in those pathways have been identifi ed, and regulation of transcription and transcription factors has been determined by studying functional genomics in conjunction with data-mining tools of bioinformatics. Besides this, advances in metabolic engineering enable researchers to confer new secondary metabolic pathways to crops by transferring three to fi ve, or more, heterologous genes taken from various other species. As an alternative, the metabolic pathways of useful secondary metabolites have been modifi ed to improve their productivity via genetic transformation. However, there is a need to understand metabolic pathways of secondary metabolism at the molecular level. Plant hairy roots offer a novel and sustainable tissue-based system that preserves multiple specialized cell types believed to be important in maintaining a better consistency in synthesis of bioactive secondary molecules. This paper will review stateof-the-art reports on improving production of secondary metabolites in tissue cultures in various plant species.

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“…Boonsnongcheep et al (2010) and Korsangruang et al (2010) have developed a system of cell suspension culture and investigated its growth and isoflavonoid accumulation for P. candollei and Thanonkeo and Panichajakul (2006) have also reported on successful callus cultures of P. candollei while remarkable efforts have been reported on cell suspension cultures and enhancement of isoflavonoid accumulation for P. tuberosa (Vaishnav et al 2006;Ramawt 2007, 2008;Rathore and Shekhawat 2009). Moreover, Sharma et al (2009Sharma et al ( , 2011 tried to scale up production of isoflavonoids in cell suspension cultures of P. tuberosa using a bioreactor. For P. lobata, a few results related to both tissue culture and isoflavone production were reported (Barbara 2003;Thiem 2003;Reppert et al 2008).…”

Section: Introductionmentioning

confidence: 99%

In vitro induction of hairy root from isoflavones-producing Korean wild arrowroot Pueraria lobata

Kim¹,

Cha²,

Lee³

et al. 2012

Journal of Plant Biotechnology

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Pueraria lobata is a perennial legume plant, widely distributed in the countries of East Asia. It is a medicinally important leguminous plant and produces various isoflavones such as puerarin, daidzein etc which have potential for preventing several chronic diseases including osteoporosis, cardiovascular disease and cancer. In this study, we tried to induce hairy roots in vitro from Korean wild arrowroot P. lobata and investigated the effects of hormones and light conditions. Initially leaf and stem segments were infected with Agrobacterium rhizogenes and incubated in different conditions. Hairy roots were induced from only stem segments and the induction was best at dark condition and the presence of IBA during incubation. Secondary roots were also significantly much more induced at the dark condition than at the 16 hours light condition. Among plant growth regulators of auxin, IBA was best for secondary root formation while 2,4-D, IAA and NAA produced callus or less hairy roots. The presence of the foreign gene rolC transferred by A. rhizogenes that plays a major role in hairy root induction was confirmed by PCR. The accumulation of isoflavones such as puerarin and daidzin was also confirmed. These results will facilitate mass production of hairy root and can be used for the production of functional substances from wild arrowroots.

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Scale up production of isoflavonoids in cell suspension cultures of Pueraria tuberosa grown in shake flasks and bioreactor

Cited by 24 publications

References 17 publications

Isoflavone production in Cyclopia subternata Vogel (honeybush) suspension cultures grown in shake flasks and stirred-tank bioreactor

Isoflavone production in Cyclopia subternata Vogel (honeybush) suspension cultures grown in shake flasks and stirred-tank bioreactor

Improving Secondary Metabolite Production in Tissue Cultures

In vitro induction of hairy root from isoflavones-producing Korean wild arrowroot Pueraria lobata

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