Scale-up production of Rehmannia glutinosa adventitious root biomass in bioreactors and improvement of its acteoside content by elicitation

Rahmat, Endang; Okello, Denis; Kim, Hyoseon; Lee, Jun; Chung, Yuseong; Komakech, Richard; Kang, Young Min

doi:10.1016/j.indcrop.2021.114059

Cited by 14 publications

(5 citation statements)

References 51 publications

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“…Although processed R. glutinosa roots have been studied extensively, there have been no studies using the roots developed biotechnologically in vitro which is the basic idea of this study. A previous study showed that R. glutinosa roots developed in vitro are safer than commercial ones since they are not contaminated by pathogens and contain higher active compounds [ 19 ].…”

Section: Discussionmentioning

confidence: 99%

Evaluation of Marker Compounds and Biological Activity of In Vitro Regenerated and Commercial Rehmannia glutinosa (Gaertn.) DC. Roots Subjected to Steam Processing

Rahmat

Chung

Nam

et al. 2022

Evidence-Based Complementary and Alternative Medicine

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Rehmannia glutinosa (Gaertn.) DC., belonging to the family Scrophulariaceae, has been known since immemorial times as a prominent oriental drug in East Asia that can treat various ailments, such as kidney disorders, anemia, and diabetes. In order to be applied for medical purposes, R. glutinosa is commonly processed using steam to increase its efficacy and biological activity. The increasing demand for R. glutinosa in the traditional medicine industry encouraged many researchers to develop a fast, efficient, and high-quality production system using biotechnological approaches. This study aimed to compare the chemical and biological activities of in vitro regenerated R. glutinosa (PKR) and commercial R. glutinosa (PCR) samples subjected to steam processing. We assessed the effects of steam processing and the differences in R. glutinosa material on 5-Hydroxymethyl-2-furaldehyde (5-HMF) content, total flavonoid and phenolic content, antioxidant activity, nitric oxide (NO) levels, and anti-inflammatory activity. PKR samples showed a significantly higher content of 5-HMF (0.15%) as compared to PCR samples (0.05%). Compared to unprocessed R. glutinosa (UPR) and PCR samples, PKR again showed the highest total phenolic and flavonoid content of 41.578 mg GAE/g and 17.208 mg RUE/g, respectively. Meanwhile, both processed R. glutinosa samples (PKR and PCR) showed a significantly higher DPPH antioxidant activity ((67.095 + 1.005)% and (61.579 + 0.907)%, respectively) than unprocessed R. glutinosa ((31.452 + 1.371)%). In addition, both PKR and PCR samples showed good anti-inflammatory activity by showing similar effects such as the inhibition of NO production and the suppression of inducible nitric oxide synthase (iNOS). Based on these results, PKR fulfilled the Chinese pharmacopeia standards, in terms of the amount of the marker compounds and showed a high level of bioactivity. Therefore, these findings are expected to be useful in verifying the efficacy of herbal medicines and the availability of suitable materials for medicinal use.

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Section: Discussionmentioning

confidence: 99%

Evaluation of Marker Compounds and Biological Activity of In Vitro Regenerated and Commercial Rehmannia glutinosa (Gaertn.) DC. Roots Subjected to Steam Processing

Rahmat

Chung

Nam

et al. 2022

Evidence-Based Complementary and Alternative Medicine

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“…10-1881305) and its productivity was verified in the larger outdoor field [13]. Moreover, our team had also developed a high quality of adventitious roots of R. glutinosa for industrial applications [14]. These materials can be used to produce high quality of processed R. glutinosa roots.…”

Section: Introductionmentioning

confidence: 92%

Standardization of Rehmannia glutinosa (Gaertn.) DC. steam processing and evaluation of its chemical, anti-oxidant, and anti-inflammatory properties

et al. 2023

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Rehmannia glutinosa (Gaertn.) DC., belonging to the family Scrophulariaceae, is an important medicinal herb cultivated in East Asia. Traditionally, R. glutinosa is steam processed to increase its efficacy in treating various ailments such as diabetes, hematinic deficiencies and adrenal disorder. However, standardization of processed R. glutinosa is highly needed to increase its quality to fulfill global market demand that is safe and possess high level of efficacy. Therefore, this study aimed to optimize the R. glutinosa steam processing methods by evaluating some key parameters such as steaming temperature, number of steaming times, steaming duration, and additive supplementation. R. glutinosa samples were steam processed at different temperatures (100 °C, 110 °C, and 120 °C), various steaming times (1 to 5 times), several steaming duration (1 to 4 h), and additives supplementation (rice wine, 5% EtOH, 10% EtOH, 20% EtOH, 30% EtOH, and 40% EtOH). As the result, 2 h, 3 replications, and supplementation with 20% EtOH at 120 °C were identified as the optimal conditions for R. glutinosa steam processing. Optimized processed R. glutinosa (SPRR 20%EtOH) resulted in significantly higher content of 5-HMF (7648.60 ± 150.08 µg/g) and iso-verbacoside (203.80 ± 10.72 µg/g) compared with unprocessed R. glutinosa (UPR). Compared to those of other samples, SPRR 20% EtOH samples had higher total flavonoid (55.36 ± 1.68 mg/g) and phenolic (69.24 ± 4.56 mg/g) contents and stronger DPPH antioxidant activity (56%). Furthermore, SPRR 20% EtOH had excellent anti-inflammatory activity, as evidenced by the suppression of inducible nitric oxide synthase (iNOS) caused by activation of nuclear factor-κB (NF-κB) through p-p65 pathway in LPS-stimulated RAW 264.7 cells. These findings will provide a basis towards industrialization of R. glutinosa processing technology that will be very helpful for oriental medication field.

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“…It has the effects of clearing heat and cooling blood, replenishing the essence and marrow, lowering blood sugar, enhancing immunity, and improving hematopoietic activity [3,4]. It is widely used to treat diseases, such as hypertension, autoimmune meningitis, osteoporosis, blood deficiency, constipation, and diabetes [5][6][7][8]. The main components of Rehmannia glutinosa are oligosaccharides, polysaccharides, and iridoid glycosides.…”

Section: Introductionmentioning

confidence: 99%

Difference analysis of oligosaccharides in different varieties of Rehmannia glutinosa

Zhao,

Song,

Cai

et al. 2024

Food Nutr. Chem.

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The oligosaccharide and monosaccharide contents of different varieties of Rehmannia glutinosa were compared to explore the difference in the oligosaccharide and monosaccharide contents in different varieties and to provide a scientific basis for formulation of product quality standards of Rehmannia glutinosa. The contents of fructose, glucose, sucrose, raffinose, and stachyose were determined via high-performance liquid chromatography. The total sugar content in the fresh Rehmannia glutinosa varieties in descending order was as follows: Beijing Tuodu 3 > Huaifeng > Jinjiu > Huaizhong 1 > Beijing 3. The total sugar content in the raw Rehmannia glutinosa varieties in descending order was as follows: Jinjiu > Huaifeng > Beijing Tuodu 3 > Beijing 3 > Huaizhong 1. The stachyose content was the highest among the five oligosaccharides. The total sugar content in the Beijing Tuodu 3 variety was the highest among the fresh Rehmannia glutinosa varieties, and the total sugar content in the Jinjiu variety was the highest among the raw Rehmannia glutinosa varieties. There were significant differences in the oligosaccharide and monosaccharide contents among the different varieties.

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Scale-up production of Rehmannia glutinosa adventitious root biomass in bioreactors and improvement of its acteoside content by elicitation

Cited by 14 publications

References 51 publications

Evaluation of Marker Compounds and Biological Activity of In Vitro Regenerated and Commercial Rehmannia glutinosa (Gaertn.) DC. Roots Subjected to Steam Processing

Evaluation of Marker Compounds and Biological Activity of In Vitro Regenerated and Commercial Rehmannia glutinosa (Gaertn.) DC. Roots Subjected to Steam Processing

Standardization of Rehmannia glutinosa (Gaertn.) DC. steam processing and evaluation of its chemical, anti-oxidant, and anti-inflammatory properties

Difference analysis of oligosaccharides in different varieties of Rehmannia glutinosa

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