Scaling up the Enzymatic Hydrolysis of Bovine Plasma Protein to Produce an Antioxidant from a Biological Source

Abstract

Background: In modern society, there is a tendency to consume products with natural origins and minimum chemical additives. This has encouraged the replacement of synthetic antioxidants for the ones obtained from natural sources, such as the antioxidants acquired from enzymatic protein hydrolysates. Objective: In this study, the process of enzymatic hydrolysis of proteins from bovine plasma, which produces hydrolysates with an antioxidant capacity (AC), was scaled up from 1 to 5 L. Methods: An experimental design was developed in 1 L to evaluate the effect of the substrate concentration (So) on the time needed to reach a Degree of hydrolysis (DH) of 20 % as well as the AC. Results: The best conditions in the 1 L reactor controlled by a Titrando 842, were transferred to 5 L in a BioFlo310 reactor. These conditions were achieved at a ratio of 80 g/L of substrate and 0.89 AU of Alcalase 2.4L/g of substrate in order to obtain level of 16.36 ± 0.21 min of the 20 % of DH and an antioxidant capacity of 58.98 ± 1.80 %. Conclusion: The results showed that while DH depends on So, the antioxidant capacity only depends on the DH. Additionally, the dimensional analysis using Re as a scaling criterion obtain allowed us to obtain the same results in the model (1 L) and the prototype (5 L).

Unraveling the biological potential of chicken viscera proteins: a study based on their enzymatic hydrolysis to obtain antioxidant peptides

“…Some authors report behaviors similar to those obtained in our work. Grimaldos and Zapata [40] studied the scale-up from 1 to 5 L bioreactor of the enzymatic hydrolysis process of bovine plasma using the Alcalase enzyme (60.4°C, E/S 10% w/w, pH 9.0) and obtained the same results of antioxidant activity.…”

Optimization and scale up of the enzymatic hydrolysis of Californian red worm protein (Eisenia foetida)

“…Therefore, the dimensioning was carried out maintaining its dynamic similarity, thus keeping the Re number constant between the laboratory system and the prototype. As established by the dimensional analysis procedure in the scale-up process, the parametric relationships of six dimensions were taken into account [ 26 , 27 ]. …”

“…In this study, the constant Reynolds number criterion has been used, which, due to its theoretical and empirical nature, determines a similarity in the hydrodynamic flow pattern. This implies that the process must maintain the same degree of homogeneity in both systems [ 27 , 46 ]. With the increase of the scale, the stirring speed required to maintain the Reynolds constant decreases significantly, with the aim of achieving similar hydrodynamic behavior in both processes [ 24 ].…”

“…The scale-up process was carried out going from a 0.5 L reactor (laboratory model) to a 7.5 L reactor (bench scale prototype), presenting a scale-up ratio of 1:15, as shown in Fig. 1 [ 27 ]. Enzymatic hydrolysis in the scale up was performed for both scales under the optimal conditions defined in the experimental design.…”