Scanning Acoustic Microscopy for Mapping the Microelastic Properties of Human Corneal Tissue

Beshtawi, Ithar; Akhtar, Riaz; Hillarby, M. C.; O’Donnell, Clare; Zhao, Xu; Brahma, Arun; Carley, Fiona; Derby, Brian; Radhakrishnan, Hema

doi:10.3109/02713683.2012.753094

Cited by 26 publications

(24 citation statements)

References 29 publications

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“…In another study performed with a 761-MHz transducer, Beshtawi et al (2013) compared c at the lm-scale of human cross-linked corneas with controls and found a significant 5% increase in c for the treated specimens. The study did not assess a or Z.…”

Section: Discussionmentioning

confidence: 95%

“…These studies revealed significant variations in the acoustic properties, e.g., in cancerous tissue or atherosclerosis, indicating that structural changes of soft tissues are manifested in the mechanical properties of tissue at the micron level. Few studies have reported SAM measurements for ocular tissues (Beshtawi et al, 2013;Marmor et al, 1977). Beshtawi et al (2013) found a 5% increase of c of corneal tissue using SAM and Marmor et al (1977) found significant variations of acoustic properties of retinal layers.…”

Section: Introductionmentioning

confidence: 97%

“…Few studies have reported SAM measurements for ocular tissues (Beshtawi et al, 2013;Marmor et al, 1977). Beshtawi et al (2013) found a 5% increase of c of corneal tissue using SAM and Marmor et al (1977) found significant variations of acoustic properties of retinal layers.…”

Section: Introductionmentioning

confidence: 97%

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Fine-resolution maps of acoustic properties at 250 MHz of unstained fixed murine retinal layers

Rohrbach

Lloyd

Silverman

et al. 2015

The Journal of the Acoustical Society of America

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Ex vivo assessment of microscale tissue biomechanical properties of the mammalian retina could offer insights into diseases such as keratoconus, and macular degeneration. A 250-MHz scanning acoustic microscope (7-μm resolution) has been constructed to derive two-dimensional quantitative maps of attenuation (α), speed of sound (c), acoustic impedance (Z), bulk modulus (B), and mass density ( ρ). The two-dimensional maps were compared to coregistered hematoxylin-and-eosin stained sections. This study is the first to quantitatively assess α, c, Z, B, and ρ of individual retinal layers of mammalian animals at high ultrasound frequencies. Significant differences in these parameters between the layers were demonstrated.

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Section: Discussionmentioning

confidence: 95%

Section: Introductionmentioning

confidence: 97%

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Fine-resolution maps of acoustic properties at 250 MHz of unstained fixed murine retinal layers

Rohrbach

Lloyd

Silverman

et al. 2015

The Journal of the Acoustical Society of America

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“…Therefore, measuring them with microscopic resolution can provide invaluable information regarding the pathogenesis of these diseases. However, very few studies have reported SAM measurements for ocular tissues [4], [16], [17]. Beshtawi et al [17] found a 5 % increase of speed of sound of cross-linked corneal tissue compared to cryosectioned corneas using SAM.…”

Section: Introductionmentioning

confidence: 99%

“…However, very few studies have reported SAM measurements for ocular tissues [4], [16], [17]. Beshtawi et al [17] found a 5 % increase of speed of sound of cross-linked corneal tissue compared to cryosectioned corneas using SAM. Marmor et al [4] found significant variations of acoustic properties of retinal layers.…”

Section: Introductionmentioning

confidence: 99%

A Novel Quantitative 500-MHz Acoustic Microscopy System for Ophthalmologic Tissues

Rohrbach

Jakob²,

Lloyd³

et al. 2017

IEEE Trans. Biomed. Eng.

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Objective This paper describes development of a novel 500-MHz scanning acoustic microscope (SAM) for assessing the mechanical properties of ocular tissues at fine resolution. The mechanical properties of some ocular tissues, such as lamina cribrosa (LC) in the optic nerve head, are believed to play a pivotal role in eye pathogenesis. Methods A novel etching technology was used to fabricate silicon-based lens for a 500-MHz transducer. The transducer was tested in a custom designed scanning system on human eyes. Two-dimensional (2D) maps of bulk modulus (K), mass density (ρ) were derived using improved versions of current state-of-the-art signal processing approaches. Results The transducer employed a lens radius of 125 μm and had a center frequency of 479 MHz with a −6-dB bandwidth of 264 MHz and a lateral resolution of 4 μm. The LC, Bruch’s membrane (BM) at the interface of the retina and choroid, and Bowman’s layer (BL) at the interface of the corneal epithelium and stroma, were successfully imaged and resolved. Analysis of the 2D parameter maps revealed average values of LC, BM and BL with KLC = 2.81 ± 0.17; GPa, KBM = 2.89 ± 0.18; GPa, K BL = 2.6 ± 0.09; GPa, ρ LC = 0.96 ± 0.03 g/cm3; ρ BM = 0.97 ± 0.04 g/cm3; ρ BL = 0.98 ± 0.04g/cm3; Significance This novel SAM was shown to be capable of measuring mechanical properties of soft biological tissues at microscopic resolution; it currently is the only system that allows Simultaneous measurement of K, ρ, and attenuation in large lateral scales (field area > 9 mm2) and at fine resolutions.

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Mechanical Heterogeneity in the Bone Microenvironment as Characterized by Atomic Force Microscopy

Chen

Hughes

Mullin

et al. 2020

Biophysical Journal

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Scanning Acoustic Microscopy for Mapping the Microelastic Properties of Human Corneal Tissue

Abstract: SAM is a novel tool for examining corneal tissue with a high spatial resolution, providing both histological and mechanical data.

Cited by 26 publications

References 29 publications

Fine-resolution maps of acoustic properties at 250 MHz of unstained fixed murine retinal layers

Fine-resolution maps of acoustic properties at 250 MHz of unstained fixed murine retinal layers

A Novel Quantitative 500-MHz Acoustic Microscopy System for Ophthalmologic Tissues

Mechanical Heterogeneity in the Bone Microenvironment as Characterized by Atomic Force Microscopy

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