Scanning Electron and Atomic Force Microscopy to Study Plasma Torch Effects on B. cereus Spores

Tarasenko, Olga; Nourbakhsh, Said; Kuo, Spencer; Bakhtina, Assya; Alusta, Pierre; Kudasheva, D.; Cowman, MK; Levón, Kalle

doi:10.1109/tps.2006.878378

Cited by 26 publications

(16 citation statements)

References 36 publications

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“…Previously, we 14 and other groups 18,35,40,57,61,64,65,67,69,72 have shown that plasma is able to destroy cells; however, it was also observed that plasma might be able to initiate or catalyze some biochemical processes in biological systems. 14,22–24,26,27 Cell death after treatment of melanoma cells with non-thermal plasma occurs via the apoptotic pathway.…”

Section: Discussionmentioning

confidence: 91%

RETRACTED ARTICLE: Non-thermal Plasma Induces Apoptosis in Melanoma Cells via Production of Intracellular Reactive Oxygen Species

et al. 2010

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Non-thermal atmospheric pressure dielectric barrier discharge (DBD) plasma may provide a novel approach to treat malignancies via induction of apoptosis. The purpose of this study was to evaluate the potential of DBD plasma to induce apoptosis in melanoma cells. Melanoma cells were exposed to plasma at doses that did not induce necrosis, and cell viability and apoptotic activity were evaluated by Trypan blue exclusion test, Annexin-V/PI staining, caspase-3 cleavage, and TUNEL® analysis. Trypan blue staining revealed that non-thermal plasma treatment significantly decreased the viability of cells in a dose-dependent manner 3 and 24 h after plasma treatment. Annexin-V/PI staining revealed a significant increase in apoptosis in plasma-treated cells at 24, 48, and 72 h post-treatment (p<0.001). Caspase-3 cleavage was observed 48 h post-plasma treatment at a dose of 15 J/cm2. TUNEL® analysis of plasma-treated cells demonstrated an increase in apoptosis at 48 and 72 h post-treatment (p<0.001) at a dose of 15 J/cm2. Pre-treatment with N-acetyl-L-cysteine (NAC), an intracellular reactive oxygen species (ROS) scavenger, significantly decreased apoptosis in plasma-treated cells at 5 and 15 J/cm2. Plasma treatment induces apoptosis in melanoma cells through a pathway that appears to be dependent on production of intracellular ROS. DBD plasma production of intracellular ROS leads to dose-dependent DNA damage in melanoma cells, detected by γ-H2AX, which was completely abrogated by pre-treating cells with ROS scavenger, NAC. Plasma-induced DNA damage in turn may lead to the observed plasma-induced apoptosis. Since plasma is non-thermal, it may be used to selectively treat malignancies.

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Section: Discussionmentioning

confidence: 91%

RETRACTED ARTICLE: Non-thermal Plasma Induces Apoptosis in Melanoma Cells via Production of Intracellular Reactive Oxygen Species

et al. 2010

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“…Melanoma cells, treated by plasma, immediately show evidence of cell necrosis--an expected outcome in the tradition of the recent trend of surface sterilization by plasma (see [35,[63][64][65][66][67][68] for example). However, the remaining cells that survived the treatment do not behave as the untreated cells do.…”

Section: Melanoma Cell Apoptotic Behaviormentioning

confidence: 94%

“…Previously it was shown by authors [16] and by other groups (see [35,[63][64][65][66][67][68][69][70][71]) that plasma is able to destroy cells; however, it was also observed that plasma might be Fig. 9 Results of observation of treated and untreated cells for a 3-day period: percent of dead cells before and after FE-DBD treatment.…”

Section: Melanoma Cell Apoptotic Behaviormentioning

confidence: 96%

Floating Electrode Dielectric Barrier Discharge Plasma in Air Promoting Apoptotic Behavior in Melanoma Skin Cancer Cell Lines

Fridman

Shereshevsky

Jost

et al. 2007

Plasma Chem Plasma Process

551

412

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“…Chemically reactive oxygen species (ROS) [4, 34-36, 39] are known to be effective in the destruction and annihilation of bacterial spores, and atomic oxygen is probably the most effective one among them in decontamination.…”

Section: Plausible Mechanismmentioning

confidence: 99%

Plasma Assisted Decontamination of Bacterial Spores

Kuo¹

2008

TOBEJ

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The efficacy and mechanism of killing bacterial spores by a plasma torch is studied. Bacterial-spore (Bacillus cereus) suspension is inoculated onto glass/paper slide-coupons and desiccated into dry samples, and inoculated into well-microplate as wet sample. The exposure distance of all samples is 4 cm from the nozzle of the torch. In the experiment, paper slide-coupon is inserted inside an envelope. The kill times on spores in three types of samples are measured to be about 3, 9, and 24 seconds. The changes in the morphology and shape of still viable spores in treated wet samples are recorded by scanning electron and atomic force microscopes. The loss of appendages and exosporium in the structure and squashed/flattened cell shape are observed. The emission spectroscopy of the torch indicates that the plasma effluent carries abundant reactive atomic oxygen, which is responsible for the destruction of spores.

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Scanning Electron and Atomic Force Microscopy to Study Plasma Torch Effects on B. cereus Spores

Cited by 26 publications

References 36 publications

RETRACTED ARTICLE: Non-thermal Plasma Induces Apoptosis in Melanoma Cells via Production of Intracellular Reactive Oxygen Species

RETRACTED ARTICLE: Non-thermal Plasma Induces Apoptosis in Melanoma Cells via Production of Intracellular Reactive Oxygen Species

Floating Electrode Dielectric Barrier Discharge Plasma in Air Promoting Apoptotic Behavior in Melanoma Skin Cancer Cell Lines

Plasma Assisted Decontamination of Bacterial Spores

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