Scanning electron microscope observations of the organic matrix in the otolith of the teleost fish Fundulus heteroclitus (Linnaeus) and Tilapia nilotica (Linnaeus)

Watabe, Norimitsu; Tanaka, Kuniaki; Yamada, Jiro; Dean, John Mark

doi:10.1016/0022-0981(82)90100-9

Cited by 57 publications

(40 citation statements)

References 4 publications

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“…Both studies showed variations in the endolymph composition, which reinforces the hypothesis of a daily variation in otolith calcification raised in previous reports (Takana et al, 1981;Mugiya, 1984Mugiya, , 1987Watabe et al, 1982;Wright et al, 1992).…”

Section: Discussionsupporting

confidence: 89%

Daily variations of endolymph composition: relationship with the otolith calcification process in trout

Borelli¹,

Guibbolini²,

Mayer-Gostan³

et al. 2003

Journal of Experimental Biology

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SUMMARYIonic and organic parameters of the otolith calcification process in the trout Oncorhynchus mykiss were analysed in plasma and endolymph over the day:night cycle. Plasma pH remained constant and total CO2concentration was significantly lower (by 21%) during the day than at night. Calcifying parameters (total CO2, total calcium concentration) were measured in the proximal and distal endolymphs and were unchanged in the latter during the day:night cycle, but fluctuated in the former. Non-collagenous protein and collagen concentrations in endolymph were higher(1.5- and 10-fold, respectively) during the day than at night. As there was no change in total calcium concentration, we propose that Ca2+increases during the dark period and was maximal by the end of the night when the total CO2 concentration has also increased (by 14%). Measurements of endolymph pH in situ revealed significant differences between samples from proximal and distal endolymph (7.38 and 7.87,respectively), but no variation between values obtained during the day and at night. Thus, the saturation state of aragonite (Sa) in the proximal endolymph should fluctuate around unity during the day:night cycle, and CaCO3precipitation should occur when supersaturation is reached. The electrophoretic pattern of proximal endolymph showed variations in both major and minor components. Immunoblotting of endolymph, using a rabbit antiserum raised against the otolith soluble organic matrix revealed an increase in the expression of two proteins (65 kDa and 75 kDa) during the day period. We propose that organic matrix and calcium carbonate deposition on the otolith vary antiphasically: organic matrix deposition begins by the end of the day period, when the concentration of organic precursors is maximal in the endolymph, whereas CaCO3 precipitation starts once the solubility of CaCO3 is exceeded.

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Section: Discussionsupporting

confidence: 89%

Daily variations of endolymph composition: relationship with the otolith calcification process in trout

Borelli¹,

Guibbolini²,

Mayer-Gostan³

et al. 2003

Journal of Experimental Biology

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“…5.0) corresponding to the growth since first capture derived from the SEM analysis. Grey values range between 0 (black) and 255 (pure white), where high values correspond to high dispersion of light due to high otolith protein content (Watabe et al 1982, Seyama et al 1991. These grey values are referred to henceforth as opacity.…”

Section: Marking Of Fish With Data Storage Tags and Srclmentioning

confidence: 99%

“…Opacity is an expression of the otolith's light refraction/dispersal capacity and is a function of protein and calcium accretion rates and protein composition, where higher protein content leads to increased opacity (Mugiya & Muramatsu 1982, Watabe et al 1982, Seyama et al 1991. The rates of calcium and protein accretion are governed by a wide range of internal and external factors, the most prominent of which are temperature (Mosegaard & Titus 1987, Volk et al 1990) and starvation (Neilson & Geen 1985), but also size and age (Morales-Nin 1986a,b, Hüssy et al 2004).…”

Section: Introductionmentioning

confidence: 99%

Using data storage tags to link otolith macro- structure in Baltic cod Gadus morhua with environmental conditions

Hüssy¹,

Nielsen²,

Mosegaard³

et al. 2009

Mar. Ecol. Prog. Ser.

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We examined otolith opacity of Baltic cod in relation to environmental conditions in order to evaluate the formation mechanisms of seasonal patterns used in age determination. Adult fish were tagged with data storage tags (DSTs) and a permanent mark was induced in the otoliths by injection of a strontium chloride solution. Based on environmental conditions experienced, fish were classified into different behavioural types: non-reproducing 'non-spawner', and 'spawner' undertaking spawning migrations. Otolith opacity, an indicator of otolith and fish somatic growth and condition, was examined in relation to these environmental drivers. Temperature was the only environmental variable with a significant effect, overlaying a strong size-related effect. The temperature effect was not uniform across behavioural types and spawning periods. Opacity showed a negative correlation with temperature as expected -but in non-spawning fish only. In spawners, the general trend was a decrease in opacity from pre-to post spawning. A significant -but positive -temperature effect was only found in the pre-spawning period. The negative effects during and following spawning were not significant. In spawners, this decoupling leads to an otolith structure with stronger contrasts and more abrupt changes, while in non-spawners, opacity changes more smoothly. The trigger for this decoupling seems to be an interaction between temperature exposure and seasonal variations in food availability and may serve as a tool to identify the occurrence and repetitiveness of spawning in Baltic cod.

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“…They are composed of calcium carbonate, mainly in the mineral form of aragonite (Carlstrom 1963) and an organic matrix in which acidic amino acids dominate (Degens et al 1969). Accretion occurs by successive depositions of incremental (calcium dominant) and discontinuous (matrix dominant) zones (Watabe et al 1982, Morales-Nin 1987. In many fish the deposition of these 2 layers occurs throughout the day, producing a recognisable 'Corresponding author.…”

Section: Introductionmentioning

confidence: 99%

Daily variations of the saccular endolymph and plasma compositions in the turbot Psetta maxima:relationship with the diurnal rhythm in otolith formation

Edeyer¹,

Pontual²,

Payan³

et al. 2000

Mar. Ecol. Prog. Ser.

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Scanning electron microscope observations of the organic matrix in the otolith of the teleost fish Fundulus heteroclitus (Linnaeus) and Tilapia nilotica (Linnaeus)

Cited by 57 publications

References 4 publications

Daily variations of endolymph composition: relationship with the otolith calcification process in trout

Daily variations of endolymph composition: relationship with the otolith calcification process in trout

Using data storage tags to link otolith macro- structure in Baltic cod Gadus morhua with environmental conditions

Daily variations of the saccular endolymph and plasma compositions in the turbot Psetta maxima:relationship with the diurnal rhythm in otolith formation

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