Scanning Electron Microscopic Examination of Yersinia enterocolitica Attached to Stainless Steel at Selected Temperatures and pH values

Herald, Paula J.; Zottola, E. A.

doi:10.4315/0362-028x-51.6.445

Cited by 47 publications

(25 citation statements)

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“…Some previous studies have reported a positive effect of lower temperature on the adhesion pattern of S. aureus. Herald and Zottola (1988) noted that Listeria monocytogenes and Yersinia enterocolitica cultivated in laboratorial media adhered to stainless surfaces in greater numbers at 21 ºC than at 30 ºC. Rode et al (2007) found higher attachment capacity for S. aureus on polystyrene when cultivated in tryptic soy broth at sub-optimal temperatures (20, 25 and 30 ºC).…”

Section: Resultsmentioning

confidence: 99%

Adhesion and biofilm formation by Staphylococcus aureus from food processing plants as affected by growth medium, surface type and incubation temperature

Jerônimo

Queiroga

Costa

et al. 2012

Braz. J. Pharm. Sci.

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This study assessed the effect of different growth media [BHI broth, BHI broth plus glucose (10 g/100 mL) and BHI broth plus NaCl (5 g/100 mL)] and incubation temperatures (28 or 37 ºC) on the adherence, detachment and biofilm formation on polypropylene and stainless steel surfaces (2 x 2 cm coupons) for a prolonged period (24-72 h) by some strains of Staphylococcus aureus (S3, S28 and S54) from food processing plants. The efficacy of the sanitizers sodium hypochlorite (250 mg/mL) and peracetic acid (30 mg/mL) in reducing the number of viable bacterial cells in a preformed biofilm was also evaluated. S. aureus strains adhered in highest numbers in BHI broth, regardless of the type of surface or incubation temperature. Cell detachment from surfaces revealed high persistence over the incubation period. The number of cells needed for biofilm formation was noted in all experimental systems after 3 days. Peracetic acid and sodium hypochlorite were not efficient in completely removing the cells of S. aureus adhered onto polypropylene and stainless steel surfaces. From these results, the assayed strains revealed high capacities to adhere and form biofilms on polypropylene and stainless steel surfaces under the different growth conditions, and the cells in biofilm matrixes were resistant to total removal when exposed to the sanitizers sodium hypochlorite and peracetic acid. Uniterms:Staphylococcus aureus/food processing. Biofilm. Surfaces. Sanitizers/efficacy.Este estudo teve como objetivo avaliar o efeito de diferentes meios de crescimento [caldo BHI, caldo BHI adicionado de glucose (10 g/100 mL) e caldo BHI adicionado de NaCl (5 g/100 mL)] e temperaturas de incubação (28 e 37 °C) sobre a adesão, separação e formação de biofilme sobre superfícies (2 x 2 cm) de polipropileno e aço inoxidável durante longo tempo de incubação (24-72 h) por parte de cepas de Staphylococcus aureus (S3, S58 e S54) isoladas de plantas de processamento de alimentos. Também foi avaliada a eficácia dos sanitizantes hipoclorito de sódio (250 mg/mL) e ácido peracético (30 mg/mL) na redução do número de células bacterianas viáveis presentes em um biofilme pré-formado. As cepas de S. aureus aderiram em número mais elevado quando incubadas em caldo BHI em ambos os tipos de superfícies e temperaturas de incubação testadas. A separação das células das superfícies revelou alta persistência ao longo do período de incubação. Número de células necessário para a formação do biofilme foi detectado depois de três dias de incubação em todos os sistemas experimentais. O ácido peracético e o hipoclorito de sódio não foram eficientes em remover completamente a células de S. aureus aderidas sobre as superfícies de polipropileno e aço inoxidável. Os resultados obtidos revelaram alta capacidade das cepas ensaiadas em aderir e formar biofilme sobre superfícies de polipropileno e aço inoxidável sobre diferentes condições de crescimento e que as células na matriz do biofilme apresentaram-se resistentes à total remoção quando expostas aos sanitizantes hipoclorito de sód...

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Section: Resultsmentioning

confidence: 99%

Adhesion and biofilm formation by Staphylococcus aureus from food processing plants as affected by growth medium, surface type and incubation temperature

Jerônimo

Queiroga

Costa

et al. 2012

Braz. J. Pharm. Sci.

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“…Adhesion phenomena has been evaluated as function of substratum, liquid medium, carbone source, pH and hydrodynamics parameters including flow rate. Many of the conclusions about biofilm development, composition, distribution, and relationship to substratum have been derived from scanning electron microscopy (Bragadeewaran et al, 2010;Herald & Zottola, 1988;Pinna et al, 2000). We report here several investigations made in our laboratory used scanning electron microscopy to study adhesion phenomena.…”

Section: Sem Applied Of Adhesion Stagementioning

confidence: 89%

Scanning Electron Microscopy (SEM) and Environmental SEM: Suitable Tools for Study of Adhesion Stage and Biofilm Formation

Abed¹,

Koraichi²,

Latrache³

et al. 2012

Scanning Electron Microscopy

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“…Pseudomonas aeruginosa and Listeria monocytogenes cells show the maximal adhesion on abiotic materials at neutral pH and a temperature of 30ºC [Herald & Zottola, 1988a;Busalmen & de Sanchez, 2001]. In Yersinia enterocolitica the adhesion is enhanced on solid surfaces at pH 8-9 and a temperature of 30ºC [Herald & Zottola, 1988b].…”

Section: Other Factorsmentioning

confidence: 93%

Bacterial Biofilms on Food Contact Surfaces - a Review

Myszka¹,

Czaczyk²

2011

Pol. J. Food Nutr. Sci.

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This review will discuss some of the basic concepts concerning biofi lm formation, development and control in the food industry. Biofi lm formation process on food contact surfaces can have a detrimental effect on the microbial status of food. The presence of biofi lm on abiotic materials can contaminate the product through direct contact. As a consequence, food spoilage is likely to occur that may lead to reduced shelf life and increased risk of food poisoning from pathogens. Bacteria colonizing food processing surfaces are extremely diffi cult to eradicate. Biofi lms can tolerate antimicrobial agents at concentrations of 10-1000 times that needed to inactivate genetically equivalent planktonic bacteria. A better understanding of bacterial adhesion process is needed for the production of microbiologically-safe and good-quality products in the food industry.

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Scanning Electron Microscopic Examination of Yersinia enterocolitica Attached to Stainless Steel at Selected Temperatures and pH values

Cited by 47 publications

References 0 publications

Adhesion and biofilm formation by Staphylococcus aureus from food processing plants as affected by growth medium, surface type and incubation temperature

Adhesion and biofilm formation by Staphylococcus aureus from food processing plants as affected by growth medium, surface type and incubation temperature

Scanning Electron Microscopy (SEM) and Environmental SEM: Suitable Tools for Study of Adhesion Stage and Biofilm Formation

Bacterial Biofilms on Food Contact Surfaces - a Review

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