— Scanning Electron Microscopic Observation of Amyloid‐Laden Mouse Liver —

Abstract

Amyloidosis of liver in mice induced by casein injection was studied by scanning electron microscopy (SEM). In the fractured‐surfaces of the central portion of amyloid nodules, they appeared as chestnut‐bur‐like structures and were composed of numerous thread‐like structures of fasciculated amyloid fibrils and of stick‐like structures of amyloid bundles in three dimensional figure. In the cytoplasm of the Kupffer cell, amyloid bundles were revealed as band‐like bulges. These bulges were projected from the Kupffer cell into the Disse's space and continued with thread‐like structures outside the cytoplasm of Kupffer cells. ACTA PATH. JAP. 27: 809‐822, 1977.

George Budd and His Contribution to the Early History of Amyloid Disease

“…microscopy (TEM) of a typical amyloid nodule, in which amyloid bundles are seen arranged in a radial fashion in the vicinity of the Kupffer cell (×12,000). Reproduced with permission from 3 Acta Pathologica Japonica 1977; 27: 809‐822. (B) Schematic representation of amyloid fibril (above left) consisting of up of 4‐6 protofilaments – each made up of contiguous ß‐sheet polypeptide chains – wound around one another to form a 7.5‐10 nm structure seen on TEM (×100,000 – below left).…”

“…Summaries of the clinical histories and presentations of Budd's cases are included below with references to his accurate analyses of the proteinaceous and not fatty nature of the infiltration, in which assays he was assisted by a certain Mr. Beale. George Budd's older brother William -the esteemed pioneer epidemiologist who had FIG 3 The Meckel family tree with the 5 anatomist/pathologists and their spouses. Reproduced with permission from 20 Neurosurgery 2010; 66:758-771. discovered the water-borne transmission of typhoid fever that he also distinguished from typhus -and a nephew, the notoriously sociopathic George Turnavine Budd, nemesis of Arthur Conan Doyle, creator of Sherlock Holmeswere also involved in the amyloid story.…”