Scanning Electron Microscopic Study on the Head Lice Eggs Detected in Korean Children

Park, Mi Soon; Chang, Byung Soo

doi:10.9729/am.2014.44.2.47

Cited by 2 publications

(1 citation statement)

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“…The prepared lice were placed on metallic stubs and vacuum-coated with gold by using SEM (JEOL Company, Model; JFC-1100E, Japan) ion sputtering device (Sputtering system: double pole horizontal electrode discharge system). The specimens were examined using electron microscopy according to Park and Chang (2014).…”

Section: Scanning Electron Microscope Of Treated Lice (Sem)mentioning

confidence: 99%

D‐limonene nanoemulsion: lousicidal activity, stability, and effect on the cuticle of Columbicola columbae

Gadelhaq

Aboelhadid

Abdel‐Baki

et al. 2022

Medical Vet Entomology

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The current study was conducted to investigate the efficacy and stability of D-limonene (DL) and its nanoemulsion (DLN) against pigeon feather lice (Columbicola columbae) and their mode of action. DL pure form and DLN were prepared and characterized freshly and after storage for 50 days. In vitro bioassay on live lice was conducted with different concentrations of DL, DLN, and deltamethrin (DM). The results revealed significant mortality rates in the DL-, DLN-, DM-treated groups when compared with the control (p < 0.05). The scanning electron micrographs of lice treated with DL and DLN revealed collapsed bodies with destruction in the cuticle of the mouthparts and damaged antennae. The 50 days stored DLN showed stability in their effectiveness when compared with the freshly prepared formulation. DL and DLN caused significant inhibition (p ≤ 0.05) in acetylcholinesterase activity (AchE). Malondialdehyde level (MDA) was significantly increased while glutathione was significantly decreased in DL-and DLNtreated lice. In conclusion, DL and DLN have significant lousicidal activities. DLN showed better stability than DL after storage for 50 days. In addition, the mode of action of DL may associate with its effect on the cuticle of the lice body, inhibition of AchE, and increasing oxidative stress in the treated lice.

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Section: Scanning Electron Microscope Of Treated Lice (Sem)mentioning

confidence: 99%