Scanning Electron Microscopy

Oatley, C.W.; Nixon, W. C. W.; Pease, R. F. W.

doi:10.1016/s0065-2539(08)61010-0

Cited by 210 publications

(42 citation statements)

References 28 publications

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“…The species come from previously unsampled regions, the quite remote (only approachable via a hike of >30 km) uniquely transitional forest of Kalambatritra (Irwin et al 2001) and its nearest neighbour, the eastern montane rainforest of Midongy-Sud (Bora et al 2007). We chose to describe both species integratively (Padial et al 2010), using drawings and scanning-electron microscopy (Oatley et al 1966) combined with genetic barcoding (Hebert et al 2003;Hebert & Gregory 2005). For only the second time in Diplopoda taxonomy (Akkari et al 2015), we also use micro-CT technology (Ritman 2004) to study and visualize internal structures non-invasively.…”

Section: R E S E a R C H A R T I C L Ementioning

confidence: 99%

Integrative description of two new species of Malagasy chirping giant pill-millipedes, genus Sphaeromimus (Diplopoda: Sphaerotheriida: Arthrosphaeridae)

Moritz‍

Wesener‍

2017

EJT

774

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Abstract. The species-rich giant pill-millipedes (Sphaerotheriida) often represent a microendemic component of Madagascar's mega-invertebrate fauna. Of the chirping genus Sphaeromimus de Saussure & Zehntner, 1902, ten species have been described. Here, we describe two new species of Sphaeromimus integratively, combining light microscopy, scanning electron microscopy, DNA barcoding and micro-CT technology for the first time in a taxonomic description of a giant pillmillipede. S. kalambatritra sp. nov. and S. midongy sp. nov. are the first giant pill-millipedes collected and described from the mountainous rainforests of Kalambatritra and Midongy. Both species show island gigantism compared to their congeners. Our analysis of the mitochondrial COI gene shows that the two species are related to one another with a moderate genetic distance (9.4%), while they are more closely related to an undetermined specimen from the forest of Vevembe (6.3% and 8.4%). They stand in a basal position with S. ivohibe S. musicus (de Saussure & Zehntner, 1897). The four aforementioned species share a high number of stridulation ribs on the male harp. Our micro-CT analysis provides a look into the head of S. kalambatritra sp. nov. and shows that non-destructive CT methods are a useful tool for studying the inner morphology of giant pill-millipedes.

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Section: R E S E a R C H A R T I C L Ementioning

confidence: 99%

Integrative description of two new species of Malagasy chirping giant pill-millipedes, genus Sphaeromimus (Diplopoda: Sphaerotheriida: Arthrosphaeridae)

Moritz‍

Wesener‍

2017

EJT

774

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“…Another reason for its usefulness stems from the large magnification range possible, which varies from about 5 times to 100,000 times [4,69,70].…”

Section: Scanning Electron Microscopementioning

confidence: 99%

Evaluation of Selected Bleaching Treatments Suitable for Historic White Cottons

Annis¹,

Reagan²

1979

Studies in Conservation

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“…The commercial scanning microscope is now a well-established scientific tool which is best developed in those instruments which use secondary electrons as the contrast mechanism (Smith & Oatley, 1955;Oatley & Pease, 1965). The focused spot of high energy electrons produces slow secondary electrons when it strikes a surface and these electrons can be collected efficiently to serve as the modulation signal for the display oscilloscope.…”

mentioning

confidence: 99%