SCANNING ELECTRON MICROSCOPY DARI Clinostomum complanatum (DIGENEA: CLINOSTOMIDAE) PADA IKAN BETOK ( Anabas testudineus ) DI YOGYAKARTA, INDONESIA

Riauwaty, Morina; Kurniasih, Kurniasih; Prastowo, Joko; Windarti, Windarti

doi:10.15578/jra.6.2.2011.303-309

Cited by 1 publication

(1 citation statement)

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“…Additionally, a distinctive characteristic was observed in the oral sucker, with C. phalacrocoracis displaying a circular shape and C. complanatum exhibiting an elliptical form. Sensory papillae and furrows were evident at the base of the tegumental surface in both species, in agreement with the findings of [ 35 ] in Indonesia, who noted an elliptical oral sucker in C. complanatum and reported the presence of sensory papillae and furrows. It’s worth noting that [ 32 ] observed wrinkles on the oral sucker of Clinostomum species, a feature they indicated was absent in other trematodes.…”

Section: Discussionsupporting

confidence: 91%

A multidisciplinary study on Clinostomum infections in Nile tilapia: micro-morphology, oxidative stress, immunology, and histopathology

Mahdy,

Abdel-Maogood,

Abdelsalam

et al. 2024

BMC Vet Res

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Yellow grub disease, caused by Clinostomum metacercaria, is an endemic zoonotic infection in freshwater fish, responsible for Halzoun syndrome transmitted through the consumption of raw infected fish. This study aimed to conduct a multidisciplinary investigation integrating detailed morphology, oxidative stress, immunology, and histopathology alteration to advance our understanding of Clinostomum infection. In this annual study, 400 Nile tilapia (Oreochromis niloticus) were collected from the Nile River at Al Bahr Al Aazam, Giza Governorate to assess Clinostomum infection prevalence. Of the examined fish, 160 individuals (40.0%) harboured larval Clinostomum infections. Clinostomum metacercariae were observed in various anatomical locations, with 135 fish (33.8%) in buccal cavities, 21 fish (5.25%) in gill chambers, and 4 fish (1.0%) on the skin. Infection intensity ranged from 2 to 12 cysts per fish, averaging 5 cysts, notably with skin infections characterized by a single cyst in each fish. Macroscopic encysted metacercariae were collected from buccal cavities, gills, and skin. Micro-morphology revealed distinct features in C. complanatum, including an elliptical oral sucker with collar-like rings and large sensory papilla-like structures, contrasting with the absence of these features in C. phalacrocoracis. Oxidative stress, assessed through malondialdehyde (MDA) and nitric oxide levels, revealed an elevation in MDA to 35.13 ± 6 nmol/g and nitric oxide to 25.80 ± 3.12 µmol/g in infected fish. In infected fish, MHC-I gene expression increased approximately 13-fold, MHC-II peaked at 19-fold, and IL-1β significantly upregulated by 17-fold, compared to control levels. Histopathology detailed associated lesions, such as cyst encapsulation and eosinophilic infiltration. Clinstomiasis and its impacts on fish hosts offer crucial insights to control this emerging fish-borne zoonotic disease, threatening wildlife, aquaculture, and human health.

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Section: Discussionsupporting

confidence: 91%