Scanning Electron Microscopy Image of Escherichia Coli Exposed with Atmospheric-Pressure Plasma Jet

Kuwahata, Hiroshi; Kumazawa, Eisaku; Ohyama, Ryu-ichiro; Itou, Atushi

doi:10.1380/ejssnt.2010.74

Cited by 4 publications

(2 citation statements)

References 8 publications

(18 reference statements)

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“…It has a gas temperature of approximately 30 °C and is a low-temperature plasma. 21) In our previous study, E. coli cells seeded on an agar medium in a Petri dish were inactivated by Ar plasma jet irradiation for 2 s. 22) In this study, we carried out an experiment to determine whether light from a plasma jet contributed to the inactivation of E. coli. In this experiment, a quartz plate with a transmittance ²95% for light with wavelengths of 200-1000 nm was inserted between the end of the quartz tube and the E. coli on the agar medium so that E. coli was irradiated with only light from the plasma jet.…”

Section: Introductionmentioning

confidence: 99%

Inactivation ofEscherichia coliusing atmospheric-pressure plasma jet

Kuwahata

Yamaguchi

Ohyama

et al. 2014

Jpn. J. Appl. Phys.

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An atmospheric-pressure argon (Ar) plasma jet was applied to the inactivation of Escherichia coli. The Ar plasma jet was generated at a frequency of 10 kHz, an applied voltage of 10 kV, and an Ar gas flow rate of 10 L/min at atmospheric pressure. E. coli cells seeded on an agar medium in a Petri dish were inactivated by Ar plasma jet irradiation for 1 s. Scanning electron microscopy (SEM) revealed that E. coli cells were killed because their cell wall and membrane were disrupted. To determine the causes of the disruption of the cell wall and membrane of E. coli, we performed the following experiments: the measurement of the surface temperature of an agar medium using a thermograph, the analysis of an emission spectrum of a plasma jet obtained using a multichannel spectrometer, and the determination of the distribution of the concentration of hydrogen peroxide (H 2 O 2 ) generated on an agar medium by plasma jet irradiation using semiquantitative test strips. Moreover, H 2 O 2 solutions of different concentrations were dropped onto an agar medium seeded with E. coli cells to examine the contribution of H 2 O 2 to the death of E. coli. The results of these experiments showed that the cell wall and membrane of E. coli were disrupted by electrons in the plasma jet, as well as by electroneutral excited nitrogen molecules (N 2 ) and hydroxyl (OH) radicals in the periphery of the plasma jet.

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Section: Introductionmentioning

confidence: 99%

Inactivation ofEscherichia coliusing atmospheric-pressure plasma jet

Kuwahata

Yamaguchi

Ohyama

et al. 2014

Jpn. J. Appl. Phys.

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“…Previously, we reported that Escherichia coli (E. coli) AB1157 on an agar medium was killed upon irradiation with an atmospheric-pressure Ar plasma jet in air for 2 s, on the basis of the proliferation of cultured E. coli and the scanning electron microscopy (SEM) observation results [12,13]. Because an SEM image of E. coli cells irradiated with a plasma jet showed that the cells were punctured but retained their original shape to a certain degree, we speculated that the structures of the cell wall and membrane were changed by Ar plasma jet irradiation.…”

Section: Introductionmentioning

confidence: 99%

Atmospheric-Pressure Plasma Jet Irradiation onto Main Components of the Cell Wall and Membrane of <i>Escherichia Coli </i>

Kuwahata

Yamaguchi

Kojima

et al. 2014

e-J. Surf. Sci. Nanotechnol.

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To clarify the mechanism underlying the inactivation of Escherichia coli (E. coli) cells subjected to atmosphericpressure argon (Ar) plasma jet irradiation, we focused on phospholipids, the main components of the cell wall and membrane of E. coli. Commercially available standard samples of phospholipids, phosphatidylethanolamine (PE) and phosphatidylglycerol (PG) were irradiated with an atmospheric-pressure Ar plasma jet in air for 5 s. The phospholipids irradiated with the plasma jet were separated by thin-layer chromatography (TLC), and the results showed that PE was unchanged even after plasma jet irradiation, whereas PG was degraded into several substances. One of them was identified as lysophosphatidylglycerol (LPG), a lysophospholipid of PG, by liquid chromatography-mass spectrometry (LC-MS). From these results, we inferred that PG in the cell wall and membrane was degraded into LPG and a fatty acid by plasma jet irradiation, and that the cell wall and membrane were disrupted, inactivating E. coli.

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Studying the non-thermal plasma jet characteristics and application on bacterial decontamination

Al-rawaf

Fuliful

Khalaf³

et al. 2018

J Theor Appl Phys

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Non-thermal atmospheric-pressure plasma jet represents an excellent approach for the decontamination of bacteria. In this paper, we want to improve and characterize a non-thermal plasma jet to employ it in processes of sterilization. The electrical characteristics was studied to describe the discharge of the plasma jet and the development of plasma plume has been characterized as a function of helium flow rate. Optical emission spectroscopy was employed to detect the active species inside the plasma plume. The inactivation efficiency of non-thermal plasma jet was evaluated against Staphylococcus aureus bacteria by measuring the diameter of inhibition zone and the number of surviving cells. The results presented that the plasma plume temperature was lower than 34 C at a flow rate of 4 slm, which will not cause damage to living tissues. The diameter of inhibition zone is directly extended with increased exposure time. We confirmed that the inactivation mechanism was unaffected by UV irradiation. In addition, we concluded that the major reasons for the inactivation process of bacteria is because of the action of the reactive oxygen and nitrogen species which formed from ambient air, while the charged particles played a minor role in the inactivation process.

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Scanning Electron Microscopy Image of Escherichia Coli Exposed with Atmospheric-Pressure Plasma Jet

Cited by 4 publications

References 8 publications

Inactivation ofEscherichia coliusing atmospheric-pressure plasma jet

Inactivation ofEscherichia coliusing atmospheric-pressure plasma jet

Atmospheric-Pressure Plasma Jet Irradiation onto Main Components of the Cell Wall and Membrane of <i>Escherichia Coli </i>

Studying the non-thermal plasma jet characteristics and application on bacterial decontamination

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