Scanning-electron microscopy of chromosome aberrations

Mace, Myles L.; Daskal, Yerach; Wray, Wayne

doi:10.1016/0027-5107(78)90141-0

Cited by 77 publications

(36 citation statements)

References 11 publications

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“…Chromosome aberrations were evaluated in 100 well spread metaphases per donor (totally 200 metaphases per concentration). Gaps were not evaluated as CA according to Mace et al (1978). The scoring of SCE was carried out according to the IPCS guidelines (Albertini et al 2000).…”

Section: Methodsmentioning

confidence: 99%

The genotoxic and anti-genotoxic effects of Stachys petrokosmos leaf extract in human lymphocytes using microsomal fractions

2011

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The genotoxic and anti-genotoxic effects of Stachys petrokosmos leaf extracts (Sp) were investigated in human lymphocytes. The cells were treated with 1.5, 3.0 and 6.0 lL/mL concentrations of Sp leaf extracts for 24 and 48 h treatment periods in the absence and presence of metabolic activator (S9mix). In the absence of S9mix, Sp alone did not induce chromosome aberrations and formation of micronucleus while inducing the mean sister chromatid exchange at the highest concentration. In addition, Sp decreased the mutagenic effect of mitomycin-c. Sp alone showed a cytotoxic effect determined by a decrease in the proliferation index, mitotic index and nuclear division index. On the other hand a mixture of Sp and mitomycin-c resulted in a higher cytotoxic effect especially for 48 h treatment period. In the presence of S9mix, Sp was not genotoxic and cytotoxic however, it showed an anti-genotoxic effect by decreasing the effects of cyclophosphamide.

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Section: Methodsmentioning

confidence: 99%

The genotoxic and anti-genotoxic effects of Stachys petrokosmos leaf extract in human lymphocytes using microsomal fractions

2011

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“…Gaps were not considered as CA according to Mace et al (1978). For each donor, 100 well-spread, intact metaphases were investigated (totaly 400 metaphase spreads for four donors) in order to score the CAs at each concentration and treatment period.…”

Section: Microscopic Evaluationmentioning

confidence: 99%

In vitro genotoxicity assessment of the synthetic plant growth regulator, 1-naphthaleneacetamide

Kocaman

Güven

2015

Cytotechnology

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1-Naphthaleneacetamide (NAAm) is a synthetic plant growth regulator in the auxin family that is widely used in agriculture to promote the growth of numerous fruits, for root cuttings and as a fruit thinning agent. The potential genotoxic effects of NAAm were investigated in vitro by the chromosome aberrations (CAs), and cytokinesis-block micronucleus assays in human peripheral blood lymphocytes (PBLs) for the first time. The human PBLs were treated with 20, 40, 80, and 160 lg/mL of NAAm for 24 and 48 h. The results of this study showed that NAAm significantly induced the formation of structural CA and MN for all concentrations (20, 40, 80 and 160 lg/mL) and treatment periods (24 and 48 h) when compared with the negative and the solvent control. In addition, the higher concentrations of NAAm (80 and 160 lg/mL) caused a statistically significant increase in nuclear bud (NBUD) formation for both 24 and 48 h treatment times. With regard to the cell cycle kinetics, at all the tested concentrations, NAAm caused a statistically significant reduction in the mitotic index (MI) only for 48 h treatment period and also in the nuclear division index (NDI) for both 24 and 48 h treatment periods as compared to the control groups. The reductions in the MI and NDI occured in a concentration-dependent manner for both treatment times. In conclusion, the present results indicate that in the tested experimental conditions, NAAm was genotoxic and cytotoxic on human PBLs in vitro.

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“…Chromatid and chromosome gaps were not evaluated as chromosome aberrations (Mace et al 1978). The significances between the percentages of means of SCE, cells with CA, the percentages of binuclear cell with MN, PI, MI, and NDI in treated cultures, and their controls were evaluated by using the t test.…”

Section: Statistical Significancementioning

confidence: 99%

In vitro genotoxic perspective of Tamiflu

İla¹,

İlhan²

2012

Cytotechnology

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The aim of this study was to investigate the genotoxic and/or cytotoxic effects of Tamiflu, commercial form of the oseltamivir antiviral and most frequently prescribed for the treatment of influenza infections, on cultured human peripheral lymphocytes by using sister chromatid exchange (SCE), chromosomal aberration (CA), and cytokinesis-blocked micronucleus (CBMN) assays. Cells were treated with 0.5, 1, 2 lg/mL oseltamivir, the Tamiflu capsule ingredient, for 24 or 48 h in the absence or presence of an exogenous metabolic activation system (S9 mix). The test chemical did not demonstrate any genotoxic effect dose-dependently but it showed a weak cytotoxicity on cells in this study. On the other hand, some concentrations of Tamiflu (2 lg/mL without S9 mix for 48 h and 1 lg/mL with S9 mix) induced SCE and also decreased significantly the proliferation index (PI) (48 h period) and the nuclear division index (NDI) (24 h period) (P \ 0.05) in the absence of S9 mix. Considering the results, Tamiflu did not induce significant increases of CA or micronucleated cells in vitro in cultured peripheral blood lymphocytes under the treatment conditions used but weak SCE induction was observed. On the other hand, the weak cytotoxic effects observed disappeared in the cultures treated in presence of the S9 mix.

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Scanning-electron microscopy of chromosome aberrations

Cited by 77 publications

References 11 publications

The genotoxic and anti-genotoxic effects of Stachys petrokosmos leaf extract in human lymphocytes using microsomal fractions

The genotoxic and anti-genotoxic effects of Stachys petrokosmos leaf extract in human lymphocytes using microsomal fractions

In vitro genotoxicity assessment of the synthetic plant growth regulator, 1-naphthaleneacetamide

In vitro genotoxic perspective of Tamiflu

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