Scanning Electron Microscopy of Lens

Abstract

Our previous transmission electron microscope and biochemical studies showed considerable regression of in utero induced galactose cataracts in rats following birth. The ultrastructural and biochemical alterations of the lens associated with such cataracts regressed considerably within a few days following birth. This report describes scanning electron microscope (SEM) observations made on lenses of offsprings of galactose-fed mothers. Lenses of rats delivered by galactose-fed mothers exhibited mature cataracts at birth. With SEM these lenses showed the presence of intercellular cysts, abnormal configuration, conformation and fragmentation of lens fibers. These changes were extensive at birth in the major portion of the lens, however, they became less extensive and gradually reduced in severity as the animal grew. By 28 days after parturition, lenses appeared normal and their morphology was comparable to that observed in lenses of offsprings from lab chow-fed animals. A probable mechanism(s) for the reestablishment of lens transparency is discussed.

Modelling cortical cataractogenesis XXIX. Calpain proteolysis of lens fodrin in cataract

“…The fluorescent and radioactive labelling studies clearly showed that in the glucosetreated rat lenses the [Ca2+]¡ level started to increase at 24 hr of incubation around the lens equatorial cortex. These data are consistent with previous studies on the localization of calcium in isolated lens cells or in the cells of the intact lens (1, [36][37][38][39][40][41]. Combined photographic observation and leakage of LDH were in agreement with the following mechanism: -pre-cataractous glucose elevation leads to lens calcium uptake.…”