Scanning Electron Microscopy of Macrophages

Carr, K. E.

doi:10.1007/978-1-4899-5031-4_6

Cited by 6 publications

(1 citation statement)

References 80 publications

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“…Thus, most of the leukocytes found to be adherent to the EC surface were probably monocytes, 8 although an unequivocal identification of this type of leukocyte cannot be based solely on scanning electron microscopy. In frozen sections, however, cells that clearly represented monocytes/ macrophages were easily identified by use of Leu-M3 and Leu-M5 antibodies, but they were rare in these sections.…”

Section: Leukocytes and Platelets On The Plaque Surfacementioning

confidence: 99%

The endothelium of advanced arteriosclerotic plaques in humans.

Bürrig

1991

Arterioscler Thromb

161

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The functional morphology of the endothelial cells (ECs) covering advanced but uncomplicated sclerotic plaques in humans was studied in carotid endarterectomy specimens and in coronary arteries from hearts explanted because of advanced ischemic heart disease. The endothelial layer was nearly always intact, and the endothelial patterns reflected the anticipated local flow patterns along the narrowed arteries, with the majority of flow irregularities downstream from the stenosis. Large (giant) ECs (defined as ECs with a surface area of S800 fim 1 ) were frequently found on the plaque surface, probably indicating accelerated EC senescence attributable to sustained nondenuding injury in the region of disturbed flow. Ultrastructurally, activation of ECs with hyperplasia of organelles was frequent In addition, as a sign of immunological activation, about 5% of ECs express class II antigens (HLA-DR and rarely focal HLA-DQ), as demonstrated by double immunofluorescence with von Willebrand factor to identify the ECs. EC activation may be responsible for adherence to the intact luminal surface by activated platelets and monocytes, which were always present (in contrast with nonsclerotic artery segments). Furthermore, an increase in myo-endothelial contacts to subendothelial modified smooth muscle cells was a regular feature of the sclerotic lesions; this feature represents an unknown process of EC and smooth muscle cell interaction in the sclerotic lesion and may be a compensatory process for EC control of smooth muscle cell proliferation. In advanced plaques the ECs are altered without denudation but with changed properties, which may contribute to plaque growth and which are consistent with the postulated EC dysfunction in the pathogenesis of arteriosclerotic lesions. (Arteriosclerosis and Thrombosis 1991; 11:1678-1689)

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Section: Leukocytes and Platelets On The Plaque Surfacementioning

confidence: 99%

The endothelium of advanced arteriosclerotic plaques in humans.

Bürrig

1991

Arterioscler Thromb

161

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Auger-Elektronen-Mikroanalyse Grundlagen und Anwendungen

Dudek¹

1986

Angewandte Oberflächenanalyse Mit SIMS Sekundär-Ionen-Massenspektrometrie AES Auger-Elektronen-Spektrometrie XPS Röntgen-Photoe

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Sensitivity of SEM/EDX to Detect Small Particles on Substrates.

Thornton¹

1997

Microsc Microanal

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The characterization of microscopic particles on substrates is of interest in numerous scientific fields. One example is semiconductor device defect review, where the characterization of particles or defects on a device is an essential aid in tracing sources of contamination in the production process. With the ever decreasing component separation on the integrated circuits the sensitivity of SEM/EDX to detect small particles on substrates is an important issue. The minimum size of particles causing defects in semiconductor device manufacture is predicted to decrease from 0.12µm today to 0.02µm by the year 2010. The question of interest is how long the SEM/EDX analysis tool can continue to be used to identify elements present in “killer” defect particles of ever decreasing size? This paper attempts to address this question.It has already been shown that films as thin as a monolayer can be detected with WDX provided high beam currents are available.

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Scanning Electron Microscopy of Macrophages

Cited by 6 publications

References 80 publications

The endothelium of advanced arteriosclerotic plaques in humans.

The endothelium of advanced arteriosclerotic plaques in humans.

Auger-Elektronen-Mikroanalyse Grundlagen und Anwendungen

Sensitivity of SEM/EDX to Detect Small Particles on Substrates.

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