Scanning Electron Microscopy of the Eggs of Aedes scapularis from Southern South America

Santos-Mallet, Jacenir Reis dos; Müller, Gerson Azulim; Gleiser, Raquel M.; Alencar, Jerônimo; Marques, William de Almeida; Sarmento, Juliana Soares; Marcondes, Carlos Brisóla

doi:10.2987/09-5959.1

Cited by 10 publications

(2 citation statements)

References 21 publications

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“…This structure is composed of two distinct layers: the endochorion and exochorion [18]. The exochorion generally shows distinctive ornamentation, which is therefore an excellent marker for distinguishing significant differences between species [19][20][21][22][23][24][25][26].…”

Section: Introductionmentioning

confidence: 99%

Aedes aegypti: egg morphology and embryonic development

et al. 2021

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Background The diseases for which Aedes aegypti is a vector are worrisome. The high vector competence of this mosquito, as well as its anthropophilia and ability to adapt to the urban environment, allows it to exploit many habitats, making its prevention an arduous task. Despite current disease control measures focused on the mosquito, the effectiveness in containing its dispersion still requires improvement; thus greater knowledge about this insect is fundamental. Methods Aedes aegypti egg morphology and embryonic development were analyzed from eggs of the insectary of the Institute of Biomedical Sciences of the University of São Paulo. Optical (light and confocal) and electronic (transmission and scanning) microscopy were used to analyze the morphological and ultrastructural features of the eggs. Embryos were observed in the initial (0–20.5 h after egg-laying), intermediate (20.6–40.1 h after egg-laying), and final (40.2–61.6 h) stages of development, and kept at a temperature of 28 °C ± 1 °C until collection for processing. Results Eggs of Ae. aegypti were whitish at the time of oviposition, and then quickly became black. The egg length was 581.45 ± 39.73 μm and the width was 175.36 ± 11.59. Access to the embryo was difficult due to the egg morphology, point of embryonic development, and difficult permeability of the exochorion (mainly in fixation). Only about 5% of the collected eggs were successfully processed. In the initial stage of embryonic development, characteristics suggestive of intense cellular activity were found. In the intermediate stage, the beginning of the segmentation process was evident. In the final phase, it was possible to differentiate the cephalic region and the thoracic and abdominal segments. Conclusion The chorion was found to be an important protective barrier and a limiting factor for the evaluation of the embryos and mosquito embryonic cells, indicating that further studies need to be carried out to identify the reason that this occurs. Graphical abstract

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Section: Introductionmentioning

confidence: 99%

Aedes aegypti: egg morphology and embryonic development

et al. 2021

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“…Aedes fluviatilis mosquitoes were recently found naturally infected by the Chikungunya virus in Natal, the capital of Rio Grande do Norte state (Ximenes et al 2020). Aedes scapularis has been found infected with the Rocio, Mayaro and Ilheus virus, among others (Consoli & Lourenço-de-Oliveira 1994;Santos-Mallet et al 2010). Aedes terrens, a species found in depressions or tree trunks, was infected experimentally with the chikungunya virus (Lourenço-De-Oliveira & Failloux 2017).…”

Section: Discussionmentioning

confidence: 99%

Mosquitoes (Diptera: Culicidae) of the Brazilian semiarid: dynamic interactions with biotic and abiotic factors

Silva-Inácio

Ximenes

2023

Austral Entomology

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Mosquitoes are found worldwide; in Brazil, 530 species are distributed across all its biomes. Understanding of the biodiversity of the Caatinga biome remains incomplete, especially for Culicidae. We carried out a sampling of immature and adult mosquitoes in aquatic habitats and using Shannon traps in a seasonally dry tropical forest, in the semiarid of Rio Grande do Norte. A total of 1747 immatures of 13 species were collected during the study period (2017–2020) in groundwater sites, rock depressions, with and without vegetation, and tree holes. The maintenance of temporary breeding sites is related to the dry and rainy seasons. We collected 2132 adult mosquitoes of 12 species between 5 and 8 PM. Correlation analyses showed the effect of meteorological variables on Culicidae populations. Both immature and adult mosquitoes' abundance were significantly influenced by temperature and wind. The abundance of adult mosquitoes of the genera Aedes demonstrated a positive correlation with temperature, and a negative one with Mansonia and Uranotaenia. Our study adds information on the population dynamics of mosquitoes in the Brazilian semiarid, highlighting the bioecological relationships and breeding sites of species relevant to public health.

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