Scanning electron microscopy of the extracellular matrices of rat molar tooth germs in organ culture in vitro

Mandibular first molars from 17-d-old mouse embryos were cultured in vitro for 2 to 4 d by a simple, disposable, improved floatation method. This method consisted of using a 24-well multidish and a plastic culture chamber with a membrane filter. The improved floatation method, as well as our previous method, was capable of the three-dimensional development of tooth germs. Cytodifferentiation of odontoblasts and ameloblasts and formation of extracellular matrices were accelerated by the present culture system, in comparison with our previous method. All the molars cultivated by this method were very similar in morphology to in vivo. On Day 2 of culture the terminal cytodifferentiation of odontoblasts and the formation of predentin were ascertained in the bucco-lingual sections of the cultured molars. A thick layer of predentin was formed at the tip of the cusp and gradually decreased toward the cervical loop and the fissure between the buccal and lingual cusps. On Day 4 in vitro, secretory ameloblasts produced enamel matrix, and the mineralized enamel showed showed prismatic structure very similar to that in vivo. Dentin and predentin also were normal in ultrastructure. The extracellular matrices (enamel, dentine, and predentin) were formed in line with the pattern of the cusp and the formation of matrices normally started at the tip of the cusp. We conclude that the three-dimensional development of whole tooth germs in vitro may be very important for normal expression of the developmental program intrinsic to mouse embryonic molars.

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A simple, disposable, and improved organ culture system for maintaining three-dimensional development of mouse embryonic molars

Sakakura

Fujiwara

Nawa

1989

In Vitro Cell Dev Biol

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Localization of lead and fluoride in cultured tooth germs by laser microprobe mass analysis

Vandeputte

Ameloot²,

Cleymaet³

et al. 1989

Biol Trace Elem Res

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Trace elements can influence dental health, possibly by altering tooth resistance during preeruptive development. Therefore, it was investigated whether lead and fluoride would be incorporated into the calcifying matrices or the cellular parts of tooth germs in vitro. Using laser microprobe mass analysis, the localization of lead and fluoride was studied in the different layers or tooth germs that had been cultured in a medium to which PbCl2 of NaF had been added in different concentrations. Both elements could only be detected in the dentine layer. Hence, the enamel organ in the secretory stage of tooth development excludes lead and fluoride from the enamel, even when enamel formation by the ameloblasts is visibly disturbed. Furthermore, there seemed to be a process of saturation in the accumulation of lead and fluoride in the dentine.

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Effect of different combinations of calcium, magnesium and phosphate on the inorganic composition of rat molars in vitro

Ameloot

Coomans

Smeyers‐Verbeke

et al. 1987

Archives of Oral Biology

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Scanning electron microscopy of the extracellular matrices of rat molar tooth germs in organ culture in vitro

Cited by 4 publications

References 30 publications

A simple, disposable, and improved organ culture system for maintaining three-dimensional development of mouse embryonic molars

A simple, disposable, and improved organ culture system for maintaining three-dimensional development of mouse embryonic molars

Localization of lead and fluoride in cultured tooth germs by laser microprobe mass analysis

Effect of different combinations of calcium, magnesium and phosphate on the inorganic composition of rat molars in vitro

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