Scanning electron microscopy of the endomysial collagen in the rat paravertebral musculature

Васильев, В. И.; Andreev, D.; Kühnel, Wolfgang

doi:10.1016/s0940-9602(11)80136-6

Cited by 3 publications

(2 citation statements)

References 2 publications

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“…The endomysium collagen fibers observed in HRSEM images show a three-dimensional net external to the sarcolemma basal lamina, and owing to the mechanical resistance of collagen, it uniformly distributes the tension forces during passive contraction and relaxation. The network between the muscle fibers and the capillaries contributes to structural and functional integration and through its wavy and loose aspect it could function as an elastic system absorbing impacts maintaining the blood flow in the muscular fiber (29).…”

Section: Discussionmentioning

confidence: 99%

Light and scanning electron microscopic studies of the angioarchitecture of intrinsic muscle fibers of the anterior rat tongue.

Motoyama

Watanabe

2001

Journal of Oral Science

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Histological and scanning electron microscopy (SEM) characteristics of the microvasculature of muscle fibers in the anterior third of the adult rat tongue are described. For light microscopy, the specimens were embedded in paraffin and frontal sections were stained with Picro-Sirius. Thick sections were obtained from Spurr embedded samples and stained with toluidine blue solution. For SEM, specimens were obtained by the corrosion cast method and fractured specimens in dimethyl sulfoxide were observed with high-resolution SEM (HRSEM). The blood vessels, enveloped with a thin layer of connective tissue, had fibers running parallel to the muscular fibers. Capillary arterioles were noted following the endomysium, but their lateral branches often ran at right angles to the fibers. The capillary beds were clearly revealed in transverse sections where the individual capillaries appeared ring-like or elliptical in shape. The nuclei of endothelial cell cores could be seen on the vessel surfaces. Collagen fibers type I and III were noted in the connective tissue and in the fibrous layer of vessels.

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Section: Discussionmentioning

confidence: 99%

Light and scanning electron microscopic studies of the angioarchitecture of intrinsic muscle fibers of the anterior rat tongue.

Motoyama

Watanabe

2001

Journal of Oral Science

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“…to Vasilev et al (1995) the endomysium is essential in shape, mechanics, and viscoelastic properties of the muscles.…”

Section: Discussionmentioning

confidence: 99%

Ultrastructure of motor nerve terminals in the anterior third of wistar rat tongue

Motoyama

Watanabe

Iyomasa

et al. 2009

Microscopy Res & Technique

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The nerve terminals of intrinsic muscular fibers of the tongue of adult wistar rats was studied by using silver impregnation techniques, transmission electron microscopy (TEM), and high resolution scanning electron microscopy (HRSEM) to observe the nerve fibers and their terminals. Silver impregnation was done according to Winkelman and Schmit, 1957. For TEM, small blocks were fixed in modified Karnovsky solution, postfixed in 1% buffered osmium tetroxide solution, and embedded in Spurr resin. For HRSEM, the parts were fixed in 2% osmium tetroxide solution with 1/15 M sodium phosphate buffer (pH 7.4) at 4 degrees C for 2 h, according to the technique described by Tanaka, 1989. Thick myelinated nerve bundles were histologically observed among the muscular fibers. The intrafusal nerve fiber presented a tortuous pathway with punctiform terminal axons in clusters contacting the surface of sarcolemma. Several myelinated nerve fibers involved by collagen fibers of the endoneurium were observed in HRSEM in three-dimensional aspects. The concentric lamellae of the myelin sheath and the axoplasm containing neurofilaments interspersed among the mitochondria were also noted. In TEM, myofibrils, mitochondria, rough endoplasmic reticulum, Golgi's apparatus, and glycogen granules were observed in sarcoplasm. It is also noted that the sarcomeres constituted by myofilaments with their A, I, and H bands and the electron dense Z lines. In areas adjacent to muscular fibers, there were myelinated and unmyelinated nerve fibers involved by endoneurium and perineurium. In the region of the neuromuscular junction, the contact with the sarcolemma of the muscular cell occurs forming several terminal buttons and showing numerous evaginations of the cell membrane. In the terminal button, mitochondria and numerous synaptic vesicles were observed.

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Mechanical and structural characteristics of single muscle fibres and fibre groups from raw and cooked pork longissimus muscle

Willems¹,

Purslow²

1997

Meat Science

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Scanning electron microscopy of the endomysial collagen in the rat paravertebral musculature

Cited by 3 publications

References 2 publications

Light and scanning electron microscopic studies of the angioarchitecture of intrinsic muscle fibers of the anterior rat tongue.

Light and scanning electron microscopic studies of the angioarchitecture of intrinsic muscle fibers of the anterior rat tongue.

Ultrastructure of motor nerve terminals in the anterior third of wistar rat tongue

Mechanical and structural characteristics of single muscle fibres and fibre groups from raw and cooked pork longissimus muscle

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