Scanning electron microscopy preparation methods: their influence on the morphology and fibril formation in <i>Pseudomonas fragi</i> (ATCC 4973)

Fraser, Thomas W.K.; Gilmour, A.

doi:10.1111/j.1365-2672.1986.tb01092.x

Cited by 28 publications

(7 citation statements)

References 10 publications

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“…Tyler & Marshall (1967) suggested that soil bacteria produce some holdfast material to give more permanent binding to the surface. Fraser & Gilmour (1986) reported that visible fibrillar material is an artefact of sample preparation, but accumulating electron microscopy data, obtained by use of different techniques in various bacteria-surface interactions, are not in accordance with this opinion (Foster, 1986).…”

Section: Discussionmentioning

confidence: 97%

Active Attachment of Azospirillum brasilense Cd to Quartz Sand and to a Light-textured Soil by Protein Bridging

Bashan¹,

Levanony²

1988

Microbiology

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Inoculation and incubation of Azospirillurn brasilense Cd in pure quartz sand resulted in their attachment to sand particles by fibrillar material. Addition of low concentrations of nutrients, such as fructose or malate together with NH,C1 or root extract, enhanced bacterial multiplication and attachment. This attachment was relatively weak, grid depended on the presence of living bacterial cells and on growth conditions. Sequential washing after bacterial attachment decreased the number of bacterial cells in the sand. The killing of A . brasifense Cd, either before or after attachment, resulted in elimination of most of the applied cells from the sand. By comparison, light-textured soil adsorbed both dead and live bacteria. Addition of protease or EDTA to attached bacteria in sand significantly reduced the number of bacteria. The addition of various inhibitors or exposure to high temperatures yielded a reduced attachment of bacteria, proportionate to their relative inhibitory effect on growth of A . brasilense Cd. Agitating A . brasilense Cd cells immediately after addition to sand reduced bacterial attachment, but increased bacterial multiplication proportionate to the increase in agitation. Attachment of A . brasilense Cd under microaerophilic conditions was lower than under aerobic conditions, and depended on the amount, quality and composition of the available nutrients. The richer the mixture, the greater the attachment and multiplication of bacteria. Similar trends, but at a lower magnitude, were observed in light-textured soil.

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Section: Discussionmentioning

confidence: 97%

Active Attachment of Azospirillum brasilense Cd to Quartz Sand and to a Light-textured Soil by Protein Bridging

Bashan¹,

Levanony²

1988

Microbiology

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“…Pseudomonas fragi (ATCC 4973), Pseudomonas fluorescens, and Acinetobacter spp. have been reported to produce biofilm (89)(90)(91)(92)(93)(94)(95)(96)(97)(98)(99)(100). Salmonella typhimurium and Yersinia enterocolitica were reported as biofilm producers at the IAMFES conference (International Association of Milk, Food, and Environmental Sanitarians) in 1990.…”

Section: Food Industrymentioning

confidence: 98%

Biofilm formation in the industry: A review

Mattila‐Sandholm

Wirtanen

1992

Food Reviews International

231

112

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“…In all investigated specimens, the bacterial cover was detached from the host surface and the bacteria appeared to adhere more firmly to one another. The mucous matrix in which the bacteria were embedded probably shrank during the preparation process [21], but is still visible as small fibers interconnecting the individual cells (Fig. 1B).…”

Section: Types and Morphology Of Bacterial Coatsmentioning

confidence: 99%

Chemoautotrophic, sulfur-oxidizing symbiotic bacteria on marine nematodes: Morphological and biochemical characterization

et al. 1992

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The marine, free-living Stilbonematinae (Nematoda: Desmodorida) inhabit the oxygen sulfide chemocline in marine sands. They are characterized by an association with ectosymbiotic bacteria. According to their ultrastructure the bacteria are Gram-negative and form morphologically uniform coats that cover the entire body surface of the worms. They are arranged in host-genus or host-species specific patterns: cocci form multilayered sheaths, rods, and crescent- or filament-shaped bacteria form monolayers. The detection of enzymes associated with sulfur metabolism and of ribulose-1,5 bisphosphate carboxylase oxygenase, as well as elemental sulfur in the bacteria indicate a chemolithoautotrophic nature of the symbionts. Their reproductive patterns appear to optimize space utilization on the host surface: vertically standing rods divide by longitudinal fission, whereas other bacteria form non-septate filaments of up to 100 μm length.

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Scanning electron microscopy preparation methods: their influence on the morphology and fibril formation in Pseudomonas fragi (ATCC 4973)

Cited by 28 publications

References 10 publications

Active Attachment of Azospirillum brasilense Cd to Quartz Sand and to a Light-textured Soil by Protein Bridging

Active Attachment of Azospirillum brasilense Cd to Quartz Sand and to a Light-textured Soil by Protein Bridging

Biofilm formation in the industry: A review

Chemoautotrophic, sulfur-oxidizing symbiotic bacteria on marine nematodes: Morphological and biochemical characterization

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