Scanning Probe Microscopy across Dimensions

Gebeshuber, Ille C.; Smith, R. A.; Winter, H.; Aumayr, F.

doi:10.1007/1-4020-3616-7_8

Cited by 1 publication

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“…Significant progress in using of atomic force microscope (AFM) as a tool for investigations of eukaryotic and prokaryotic cells has been reached during past decade [ 1 , 2 ]. In contrast to traditional methods of visualization—scanning electron and optical microscopy—atomic force microscopy offers important benefits: high spatial resolution, real quantitative data acquisition in three dimensions, relatively simple and nondestructive sample preparation procedure, and flexibility in ambient operating conditions (i.e., without the need for a vacuum or gold sputtering) [ 3 ].…”

Section: Introductionmentioning

confidence: 99%

Humidity-Dependent Bacterial Cells Functional Morphometry Investigations Using Atomic Force Microscope

Nikiyan

Vasilchenko

Deryabin

2010

International Journal of Microbiology

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The effect of a relative humidity (RH) in a range of 93–65% on morphological and elastic properties of Bacillus cereus and Escherichia coli cells was evaluated using atomic force microscopy. It is shown that gradual dehumidification of bacteria environment has no significant effect on cell dimensional features and considerably decreases them only at 65% RH. The increasing of the bacteria cell wall roughness and elasticity occurs at the same time. Observed changes indicate that morphological properties of B. cereus are rather stable in wide range of relative humidity, whereas E. coli are more sensitive to drying, significantly increasing roughness and stiffness parameters at RH ≤ 84% RH. It is discussed the dependence of the response features on differences in cell wall structure of gram-positive and gram-negative bacterial cells.

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Section: Introductionmentioning

confidence: 99%