Scanning tunneling microscopy of unbroken chloroplasts

Dahn, Douglas C.; Cake, K.M.; Hale, Lawrence R.

doi:10.1016/0304-3991(92)90427-l

Cited by 8 publications

(3 citation statements)

References 9 publications

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“…Fibrous structures with a height of 1.2 nm and a width of 13 nm were also observed. Taking convolution effects into account (see Supporting Information), [22] the actual width of the fibrous structures is 8 nm. The surface coverage of the fibrous structure increased with increasing holding time and became dominant at 0.2 h. These fibrous www.chemeurj.org structures are considered to be of monolayer thickness, based on the diameter of the fullerene.…”

Section: Resultsmentioning

confidence: 99%

Fullerene Nanowires: Self‐Assembled Structures of a Low‐Molecular‐Weight Organogelator Fabricated by the Langmuir–Blodgett Method

Tsunashima

Noro

Akutagawa

et al. 2008

Chemistry A European J

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Fullerene derivative C60TT, which is substituted with the low-molecular-weight organogelator tris(dodecyloxy)benzamide, formed nanowire structures on application of the Langmuir-Blodgett (LB) method. The surface morphology of the C60TT LB film was dependent on the holding time before deposition at a surface pressure of 5 mN m(-1); it changed from a homogeneous monolayer to a bilayer fibrous structure via a fibrous monolayer structure, which was estimated to have dimensions of 1.2 nm in height, 8 nm in width, and 5-10 microm in length. From the structural and spectroscopic data, it is inferred that close packing of the fullerene moiety occurs along with intermolecular hydrogen bonding within the monolayer fibrous structure. The morphological changes in the LB film are explained kinetically by the Avrami theory, based on the decrease in the surface area of the monolayer at the air/water interface. The growth of the quasi-one-dimensional fibrous monolayer structures at holding times from 0 to 0.2 h is considered to be an interface-controlled process, whereas the growth of the quasi-one-dimensional bilayer fibrous structures from 0.2 to 18 h is thought to be a diffusion-controlled process.

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Section: Resultsmentioning

confidence: 99%

Fullerene Nanowires: Self‐Assembled Structures of a Low‐Molecular‐Weight Organogelator Fabricated by the Langmuir–Blodgett Method

Tsunashima

Noro

Akutagawa

et al. 2008

Chemistry A European J

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“…11 Optical microscopy techniques, such as fluorescence microscopy, provide some opportunities to access chemical fluxes from individual cells (or components) but this is indirect, requiring the use of appropriate indicators. 12,13 In terms of flux measurements, scanning electrochemical microscopy (SECM) is particularly suited to detect a wide range of redox-active species with high spatial and temporal resolution.…”

Section: Introductionmentioning

confidence: 99%

“…Microscopy investigations of individual chloroplasts and thylakoid membranes have tended to focus on structure, rather than the flux of chemical species, using techniques such as optical microscopy, − electron microscopy, − atomic force microscopy, , or scanning tunneling microscopy . Optical microscopy techniques, such as fluorescence microscopy, provide some opportunities to access chemical fluxes from individual cells (or components), but this is indirect, requiring the use of appropriate indicators. , …”

Section: Introductionmentioning

confidence: 99%

Quantitative Local Photosynthetic Flux Measurements at Isolated Chloroplasts and Thylakoid Membranes Using Scanning Electrochemical Microscopy (SECM)

et al. 2013

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Quantitative local photosynthetic flux measurements at isolated chloroplasts and thylakoid membranes using scanning electrochemical microscopy (SECM). Copies of full items can be used for personal research or study, educational, or not-forprofit purposes without prior permission or charge. Provided that the authors, title and full bibliographic details are credited, a hyperlink and/or URL is given for the original metadata page and the content is not changed in any way. Publisher's statement:This document is the Accepted Manuscript version of a Published Work that appeared in final form in Journal of Physical Chemistry Part B, copyright © American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see http://dx.doi.org/10.1021/jp403048fThe version presented here may differ from the published version or, version of record, if you wish to cite this item you are advised to consult the publisher's version. Please see the 'permanent WRAP url' above for details on accessing the published version and note that access may require a subscription. (CN) 6 4-), from the artificial electron acceptor ferricyanide (Fe(CN) 6 3-), using a stationary ultramicroelectrode at chloroplasts and thylakoid membranes (sourced from chloroplasts). Oxygen generation at films of chloroplasts and thylakoid membranes was detected directly during photosynthesis, but in the case of thylakoid membranes this switched to sustained oxygen consumption at longer illumination times. An initial oxygen concentration spike was detected over both chloroplast and thylakoid membrane films, and the kinetics of the oxygen generation were extracted by fitting the experimental data to a finite element method (FEM) simulation. In contrast to previous work, the oxygen generation spike was attributed to the limited size of the plastoquinone pool, a key component in the linear electron transport pathway and a contributing factor in photoinhibition. Finally, the mobile nature of the SECM probe, and its high spatial resolution, also allowed us to detect ferrocyanide produced from a single thylakoid membrane. These results further demonstrate the power of SECM for localized flux measurements in biological processes, in this case photosynthesis, and, that the high time resolution, combined with FEM simulations, allows the elucidation of quantitative kinetic information.

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Nanoscale Characterization of Surfaces and Interfaces

DiNardo

2006

Materials Science and Technology

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Scanning tunneling microscopy of unbroken chloroplasts

Cited by 8 publications

References 9 publications

Fullerene Nanowires: Self‐Assembled Structures of a Low‐Molecular‐Weight Organogelator Fabricated by the Langmuir–Blodgett Method

Fullerene Nanowires: Self‐Assembled Structures of a Low‐Molecular‐Weight Organogelator Fabricated by the Langmuir–Blodgett Method

Quantitative Local Photosynthetic Flux Measurements at Isolated Chloroplasts and Thylakoid Membranes Using Scanning Electrochemical Microscopy (SECM)

Nanoscale Characterization of Surfaces and Interfaces

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