Scano-magneto immunoassay based on carbon nanotubes/gold nanoparticles nanocomposite for Salmonella enterica serovar Typhimurium detection

Amaro, Moegiratul; Oaew, Sukunya; Surareungchai, Werasak

doi:10.1016/j.bios.2012.05.018

Cited by 22 publications

(7 citation statements)

References 66 publications

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“…The results showed that if the plasma was not diluted, the LOD only reached 0.4 ng/mL, while for diluted plasma the LOD reached 0.1 ng/mL ( Figure 2A ). The difference may be due to interference by proteins or lipids in the plasma ( 15 ).…”

Section: Resultsmentioning

confidence: 99%

“…The highly variable natures of these different food matrices pose a challenge to BoNTs detection ( 3 , 12 ). For antigen-antibody reactions, it has previously been reported that the acidity of freshly squeezed orange juice and the fat or casein in milk can affect antigen-antibody binding, which could negatively affect the assay ( 15 ). To reduce the effects of the food matrix, some studies isolated, tested, and quantified toxins from food samples ( 3 , 16 ).…”

Section: Discussionmentioning

confidence: 99%

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Rapid and sensitive detection of botulinum toxin type A in complex sample matrices by AlphaLISA

Zhang

Lv²,

Zheng³

et al. 2022

Front. Public Health

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Botulinum toxin A(BoNT/A) is a neurotoxin produced by the bacteria Clostridium botulinum, which can cause serious food poisoning and is recognized as a potential biological warfare agent. BoNT/A is does not degrade easily and can remain in the complex matrix for a long time. Meanwhile, the poisonous dose of botulinum toxin exceptionally low and intravenous human lethal doses estimated at 1-3 ng/kg. Therefore, sensitive and accurate detection methods suitable for testing a wide range of complex samples are urgently needed. To this end, the “amplified luminescent proximity homogeneous assay linked immunosorbent assay” (AlphaLISA) was established for the detection of BoNT/A and its detection efficacy in plasma, beverage, food, and other complex samples was evaluated. The results showed that this method can very effectively resist matrix interference. The detection time is rapid, reaching a detection limit for all samples of up to 0.1 ng/mL in only 30 min. BoNT/A can also be accurately detected in vomit samples of patients with clinical food poisoning. This study demonstrates that AlphaLISA is an effective tool for the detection of BoNT/A in complex samples and can potentially be developed for commercial use in the future.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Rapid and sensitive detection of botulinum toxin type A in complex sample matrices by AlphaLISA

Zhang

Lv²,

Zheng³

et al. 2022

Front. Public Health

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show abstract

“…Another study using an immunochromatographic strip assay combined with signal amplification using liposome vesicles showed the detection limit to be 100 CFU/mL (Shukla et al, 2011). Other immunoassays reported lower detection limits of 10-50 CFU/mL (Amaro et al, 2012;Chattopadhyay et al, 2013). However, these methods require longer assay times, or more expensive and labor-intensive assay platform fabrication and/or sample preparation steps and expensive instrumentation.…”

Section: Sensitivity Of Imb-based Fluorescence Assaymentioning

confidence: 92%

“…Laube et al used phagoomagnetic immunoassay to detect S. Typhimurium in LB broth and milk, and obtained detection limits of 10 and 19 CFU/mL without any pre-enrichment step, respectively, which indicates the sensitivity of the assay decreased by nearly twofold in food testing (Laube et al, 2014). Similarly, Amaro et al detected S. Typhimurium in various food samples, including chicken broth, low fat milk and freshly squeezed orange juice, using scano-magneto immunoassay based on a nano-composite (Amaro et al, 2012). They found the sensitivity of the assay significantly decreased with food samples with detection limits of 838 CFU/mL (chicken broth), 3.2 3 10 3 CFU/mL (low fat milk), 3.7 3 10 4 CFU/ mL (orange juice) and 3.7 3 10 4 CFU/mL (whole milk), compared to 42 CFU/mL in buffer.…”

Section: Detection Of Salmonella In Food Samplesmentioning

confidence: 96%

Magnetic Bead‐Based Immunoassay Coupled with Tyramide Signal Amplification for Detection of Salmonella in Foods

Herzig

Aydın

Dunigan

et al. 2016

Journal of Food Safety

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Salmonella is the leading cause of bacteria-associated foodborne illnesses in the United States. Early detection of this pathogen by a rapid and sensitive assay is important to prevent salmonellosis. In this study, we describe a magnetic beadbased immunoassay for detection of Salmonella consisting of immunomagnetic separation for simple target concentration with tyramide signal amplification to increase the assay sensitivity. The developed immunoassay was able to detect Salmonella Typhimurium in culture with the detection limit of 280 CFU/mL in less than 3 h without any enrichment and further decreased to 70 CFU/mL with 3 h enrichment. When tested with ground beef and poultry samples artificially contaminated with S. Typhimurium and Enteritidis, the assay showed increased detection limits with 800 and 200 CFU/mL, respectively, due to the effect of complex food matrices. However, when 12 h enrichment was added, the detection limits in both food matrices decreased to 1 CFU. PRACTICAL APPLICATIONSThis study demonstrated that using immunomagnetic separation (IMS) and tyramide signal amplification can enhance sensitivity and specificity for the detection of Salmonella in food samples. The developed assay has great potential as a simple monitoring system for foodborne pathogens in food samples, which can improve food safety and public health. The results were also compared with sandwich type enzyme-linked immunosorbent assay, which showed the developed assay is approximately 50 times more sensitive than enzyme-linked immunosorbent assay.

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“…The transducers convert detectable biological recognition signals such as color change, fluorescence signal, and electrochemical signals. Nanomaterials such as gold nanoparticle (AuNP) [ 20 , 21 ], magnetic nanoparticle (MNPs) [ 22 ], quantum dot (QDs), carbon nanotubes (CNTs) [ 23 , 24 , 25 ], and carbon nanotubes with coupled gold nanoparticles (MWCNTs/AuNPs) [ 26 ] are the signal transduction elements [ 16 ].…”

Section: Introductionmentioning

confidence: 99%

Nucleic Acid-Based Nanobiosensor (NAB) Used for Salmonella Detection in Foods: A Systematic Review

et al. 2022

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Salmonella bacteria is a foodborne pathogen found mainly in food products causing severe symptoms in the individual, such as diarrhea, fever, and abdominal cramps after consuming the infected food, which can be fatal in some severe cases. Rapid and selective methods to detect Salmonella bacteria can prevent outbreaks when ingesting contaminated food. Nanobiosensors are a highly sensitive, simple, faster, and lower cost method for the rapid detection of Salmonella, an alternative to conventional enzyme-linked immunosorbent assay (ELISA) and polymerase chain reaction (PCR) techniques. This study systematically searched and analyzed literature data related to nucleic acid-based nanobiosensors (NABs) with nanomaterials to detect Salmonella in food, retrieved from three databases, published between 2010 and 2021. We extracted data and critically analyzed the effect of nanomaterial functionalized with aptamer or DNA at the limit of detection (LOD). Among the nanomaterials, gold nanoparticles (AuNPs) were the most used nanomaterial in studies due to their unique optical properties of the metal, followed by magnetic nanoparticles (MNPs) of Fe3O4, copper nanoparticles (CuNPs), and also hybrid nanomaterials multiwalled carbon nanotubes (c-MWCNT/AuNP), QD/UCNP-MB (quantum dotes upconverting nanoparticle of magnetic beads), and cadmium telluride quantum dots (CdTe QDs@MNPs) showed excellent LOD values. The transducers used for detection also varied from electrochemical, fluorescent, surface-enhanced Raman spectroscopy (SERS), RAMAN spectroscopy, and mainly colorimetric due to the possibility of visualizing the detection result with the naked eye. Furthermore, we show the magnetic separation system capable of detecting the target amplification of the genetic material. Finally, we present perspectives, future research, and opportunities to use point-of-care (POC) diagnostic devices as a faster and lower cost approach for detecting Salmonella in food as they prove to be viable for resource-constrained environments such as field-based or economically limited conditions.

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Scano-magneto immunoassay based on carbon nanotubes/gold nanoparticles nanocomposite for Salmonella enterica serovar Typhimurium detection

Cited by 22 publications

References 66 publications

Rapid and sensitive detection of botulinum toxin type A in complex sample matrices by AlphaLISA

Rapid and sensitive detection of botulinum toxin type A in complex sample matrices by AlphaLISA

Magnetic Bead‐Based Immunoassay Coupled with Tyramide Signal Amplification for Detection of Salmonella in Foods

Nucleic Acid-Based Nanobiosensor (NAB) Used for Salmonella Detection in Foods: A Systematic Review

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