The fauna of Phytoseiidae in Ruyuan Yao Autonomous County, in China is still poorly known. A survey of these mites was conducted in November 10–15, 2021, collecting 32 species, three of which are new to science. The species collected belong to nine genera of all three phytoseiid subfamilies. A checklist of the species collected is provided, including the description of the new species, named Euseius hamiltonii sp. nov., Phytoseius subcapitatus sp. nov. and Typhlodromus ruyuanensis sp. nov.