SCAR Marker for Identification and Discrimination of <i>Commiphora wightii</i> and<i> C. myrrha</i>

Sairkar, Pramod; Sharma, Anjana; Shukla, Narmada Prasad

doi:10.1155/2016/1482796

Cited by 12 publications

(6 citation statements)

References 71 publications

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“…This suggest that cloning-based sequencing is a preferred method to convert from RAPD to SCAR marker and it has been used by previous research groups in various plant such as longan (Yang et al, 2013); Commiphora sp. (Sairkar et al, 2016). The obtained sequences were then searched for homology by BLAST analysis.…”

Section: Resultsmentioning

confidence: 99%

Development of SCAR makers for longan (Dimocarpus longan L.) authentication in Vietnam

Ho¹,

Ngo²

2018

bta

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Longan (Dimocarpus longan L.) is an important fruit plant in Vietnam and therefore the demand of genetic conservation and variety authentication is of high importance. Due to the limitations of chemical and morphological longan characterization approaches, it is necessary to develop more precise DNA-based methods. In this study, we focused on developing SCAR (Sequence Characterized Amplified Region) markers for longan identification. The total of 11 longan accessions collected from different provinces in Vietnam were genetically characterized by using 20 RAPD (Random Amplified Polymorphic DNA) primers to identify polymorphic RAPD fragments. Twenty two polymorphic RAPD fragments were obtained and 2 of them were further sequenced and developed into SCAR markers. The sequences of specific RAPD fragments were used to design primers. Totally, 2 pairs of SCAR primers were archived. These primers showed significant effectiveness for cultivar identification of 2 favourite longan cultivars of Vietnam namely "Duong phen Hung Yen" and "Chin muon Hung Yen".

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Section: Resultsmentioning

confidence: 99%

Development of SCAR makers for longan (Dimocarpus longan L.) authentication in Vietnam

Ho¹,

Ngo²

2018

bta

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“…Authentication of endangered plants, like Michelia coriacea [29], Commiphora spp. [30], and Physalis [31], has also been carried out earlier using SCAR markers.…”

Section: Discussionmentioning

confidence: 99%

Development of an In Vitro Propagation Protocol and a Sequence Characterized Amplified Region (SCAR) Marker of Viola serpens Wall. ex Ging

Jha

Naz

Asif

et al. 2020

Plants

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An efficient protocol of plant regeneration through indirect organogenesis in Viola serpens was developed in the present study. Culture of leaf explants on MS (Murashige and Skoog) medium supplemented with 2.0 mg/L 6-benzyladenine and 0.13 mg/L 2,4-dichloro phenoxy acetic acid. Adventitious shoot formation was observed when calli were transferred on to MS medium containing 0.5 mg/L α-naphthalene acetic acid and 2.25 mg/L kinetin, which showed the maximum 86% shoot regeneration frequency. The highest root frequency (80.92%) with the 5.6 roots per explant and 1.87 cm root length was observed on MS medium supplemented with 2 mg/L indole-3-butyric acid. The plantlets were transferred to the mixture of sand, coffee husk and soil in the ratio of 1:2:1 in a pot, and placed under 80% shade net for one month. It was then transferred to 30% shade net for another one month, prior to transplantation in the field. These plantlets successfully acclimatized under field conditions. A Sequence Characterized Amplified Region (SCAR) marker was also developed using a 1135 bp amplicon that was obtained from RAPD (Random Amplification of Polymorphic DNA) analysis of six accessions of V. serpens. Testing of several market samples of V. serpens using the SCAR marker revealed successful identification of the genuine samples of V. serpens. This study, therefore, provides a proficient in vitro propagation protocol of V. serpens using leaf explants and a SCAR marker for the authentic identification of V. serpens. This study will be helpful for conservation of authentic V. serpens.

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“…Of course, in the future, we will collect more Physalis species to further verify the reliability of our results. An increasing number of studies have reported that SCAR markers have been used to authenticate a variety of plant species, such as Panax japonicas ( Choi et al, 2008 ), Casuarina equisetifolia ( Ghosh et al, 2011 ), Jatropha curcas ( Mulpuri et al, 2013 ), Rosa ( Bashir et al, 2014 ), Commiphora ( Sairkar et al, 2016 ), and Dasypyrum species ( Hu et al, 2017 ).…”

Section: Discussionmentioning

confidence: 99%

Development of Species-Specific SCAR Markers, Based on a SCoT Analysis, to Authenticate Physalis (Solanaceae) Species

Feng

Zhu

et al. 2018

Front. Genet.

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Physalis is an important genus in the Solanaceae family. It includes many species of significant medicinal value, edible value, and ornamental value. However, many Physalis species are easily confused because of their similar morphological traits, which hinder the utilization and protection of Physalis resources. Therefore, it is necessary to create fast, sensitive, and reliable methods for the Physalis species authentication. Intended for that, in this study, species-specific sequence-characterized amplified region (SCAR) markers were developed for accurate identification of the closely related Physalis species P. angulata, P. minima, P. pubescens, and P. alkekengi var. franchetii, based on a simple and novel marker system, start codon targeted (SCoT) marker. A total of 34 selected SCoT primers yielded 289 reliable SCoT loci, of which 265 were polymorphic. Four species-specific SCoT fragments (SCoT3-1404, SCoT3-1589, SCoT5-550, and SCoT36-520) from Physalis species were successfully identified, cloned, and sequenced. Based on these selected specific DNA fragments, four SCAR primers pairs were developed and named ST3KZ, ST3MSJ, ST5SJ, and ST36XSJ. PCR analysis of each of these primer pairs clearly demonstrated a specific amplified band in all samples of the target Physalis species, but no amplification was observed in other Physalis species. Therefore, the species-specific SCAR primer pairs developed in this study could be used as powerful tools that can rapidly, effectively, and reliably identify and differentiate Physalis species.

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SCAR Marker for Identification and Discrimination of Commiphora wightii and C. myrrha

Cited by 12 publications

References 71 publications

Development of SCAR makers for longan (Dimocarpus longan L.) authentication in Vietnam

Development of SCAR makers for longan (Dimocarpus longan L.) authentication in Vietnam

Development of an In Vitro Propagation Protocol and a Sequence Characterized Amplified Region (SCAR) Marker of Viola serpens Wall. ex Ging

Development of Species-Specific SCAR Markers, Based on a SCoT Analysis, to Authenticate Physalis (Solanaceae) Species

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