Scarce detection of mobile erm genes associated with tetQ in Bacteroides and Parabacteroides from Costa Rica

Quesada-Gómez, Carlos; Rodríguez-Cavallini, Evelyn; Rodríguez, César

doi:10.1016/j.anaerobe.2013.03.006

Cited by 8 publications

(6 citation statements)

References 35 publications

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“…After the debut of Tc in the 1950s, few isolates were resistant. By the 1970s, 20 to 30% of clinical and community isolates were Tc resistant, and by the 1990s, over 80% of Bacteroides isolates from clinical and community sources were resistant to Tc (20–23). Resistance to Tc has become so commonplace that Bacteroides strains are often not tested for susceptibility and are assumed to be resistant (24, 25).…”

Section: Introductionmentioning

confidence: 99%

Regulation of CTnDOT Conjugative Transfer Is a Complex and Highly Coordinated Series of Events

Waters

Salyers

2013

mBio

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CTnDOT is a 65-kb conjugative transposon that is found in Bacteroides spp., which are one of the more abundant members within the lower human gastrointestinal tract. CTnDOT encodes resistance to the antibiotics erythromycin and tetracycline (Tc). An interesting feature of CTnDOT is that exposure to low levels of Tc induces a cascade of events that ultimately results in CTnDOT conjugative transfer. However, Tc is apparently not a switch that activates transfer but rather a signal that appears to override a series of negative regulators that inhibit premature excision and transfer of CTnDOT. In this minireview, we summarize over 20 years of research that focused on elucidating the highly coordinated regulation of excision, mobilization, and transfer of CTnDOT.

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Section: Introductionmentioning

confidence: 99%

Regulation of CTnDOT Conjugative Transfer Is a Complex and Highly Coordinated Series of Events

Waters

Salyers

2013

mBio

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“…ermG was the only gene found in three strains (B. fragilis and Prevotella denticola). In previous studies in Costa Rica done with strains isolated from human samples, clindamycin resistance was not associated to erm genes [37].…”

Section: A C C E P T E D Accepted Manuscriptmentioning

confidence: 78%

Identification and antimicrobial susceptibility of obligate anaerobic bacteria from clinical samples of animal origin

et al. 2015

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The etiology of veterinary infectious diseases has been the focus of considerable research, yet relatively little is known about the causative agents of anaerobic infections. Susceptibility studies have documented the emergence of antimicrobial resistance and indicate distinct differences in resistance patterns related to veterinary hospitals, geographic regions, and antibiotic-prescribing regimens. The aim of the present study was to identify the obligate anaerobic bacteria from veterinary clinical samples and to determinate the in vitro susceptibility to eight antimicrobials and their resistance-associated genes. 81 clinical specimens obtained from food-producing animals, pets and wild animals were examined to determine the relative prevalence of obligate anaerobic bacteria, and the species represented. Bacteroides spp, Prevotella spp and Clostridium spp represented approximately 80% of all anaerobic isolates. Resistance to metronidazole, clindamycin, tetracycline and fluoroquinolones was found in strains isolated from food-producing animals. Ciprofloxacin, enrofloxacin and cephalotin showed the highest resistance in all isolates. In 17%, 4% and 14% of tetracycline-resistant isolates, the resistance genes tetL, tetM and tetW were respectively amplified by PCR whereas in 4% of clindamycin-resistant strains the ermG gene was detected. 26% of the isolates were positive for cepA, while only 6% harbored the cfxA (resistance-conferring genes to beta-lactams). In this study, the obligate anaerobic bacteria from Costa Rica showed a high degree of resistance to most antimicrobials tested. Nevertheless, in the majority of cases this resistance was not related to the resistance acquired genes usually described in anaerobes. It is important to address and regulate the use of antimicrobials in the agricultural industry and the empirical therapy in anaerobic bacterial infections in veterinary medicine, especially since antibiotics and resistant bacteria can persist in the environment.

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“…Parabacteroides sp . is an anaerobic bacteria and prefers oxygen‐poor environments. Additionally, the species has been isolated from the wastewater of a paper mill .…”

Section: Resultsmentioning

confidence: 99%

“…Parabacteroides sp. is an anaerobic bacteria 35 and prefers Table 3. Sequence analysis of the selected DGGE bands.…”

Section: Identification Of Dominant Species In the Agsmentioning

confidence: 99%

Effects of influent loads on performance and microbial community dynamics of aerobic granular sludge treating piggery wastewater

Chen

Huang

Tian

et al. 2017

J of Chemical Tech & Biotech

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BACKGROUND Understanding how influent loads affect the microbial community may promote applications of aerobic granular sludge (AGS). Herein, performance and microbial community dynamics of the AGS were assessed with stepwise increasing influent loads. RESULTS AGS exhibited good structure stability and effective removal efficiency; when the influent load increased from 6.5 kg COD m‐3 d‐1 (0.255 NH4+‐N m‐3 d‐1) to 10.1 kg COD m‐3 d‐1 (0.455 kg NH4+‐N m‐3 d‐1). COD and NH4+‐N removal efficiencies reached 97.1–97.9% and 84.5–98%, respectively. As indicated from the PCR‐DGGE results, the microbial community and dominant species in the AGS also gradually evolved along with the increase of influent loads. A coincidence was observed, namely, three main distinct clusters of the phylogenetic tree appeared respectively in the three stages of influent loads. The microbial diversity attained a maximum in the medium loads stage. The identified bacteria mainly belonged to Proteobacteria, Actinobacteria, Bacteroidetes and Firmicutes. The dominant species were responsible for the AGS stability and pollutant removal. CONCLUSION Variation in the influent load strongly affected the microbial community dynamics, and hence the AGS performance. These results and understanding could lead to potential improvements in guiding AGS wastewater treatment. © 2017 Society of Chemical Industry

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Scarce detection of mobile erm genes associated with tetQ in Bacteroides and Parabacteroides from Costa Rica

Cited by 8 publications

References 35 publications

Regulation of CTnDOT Conjugative Transfer Is a Complex and Highly Coordinated Series of Events

Regulation of CTnDOT Conjugative Transfer Is a Complex and Highly Coordinated Series of Events

Identification and antimicrobial susceptibility of obligate anaerobic bacteria from clinical samples of animal origin

Effects of influent loads on performance and microbial community dynamics of aerobic granular sludge treating piggery wastewater

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