Scheduled simple production method of pseudopregnant female mice for embryo transfer using the luteinizing hormone-releasing hormone agonist

Sari, Gema Puspa; Hilario, Patrick Louis Lagman; Yuri, Shunsuke; Honda, Arata; Isotani, Ayako

doi:10.1038/s41598-022-26425-2

Cited by 3 publications

(3 citation statements)

References 44 publications

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“…One possible solution to this issue is using a deep learning-based method (Sano et al, 2020). In addition, combining our method and some studies on synchronizing the estrus cycle by hormone administration may be effective in producing pseudopregnancy (Hasegawa et al, 2017;Sari et al, 2022). Another approach for enhancing pregnancy rates involves monitoring ovulation and the thickness of the fallopian tubes or uterus.…”

Section: Discussionmentioning

confidence: 99%

Intraperitoneal administration of mouse kisspeptin‐10 to mice during estrus stage induces pseudopregnancy

Mashiko,

Tokoro,

Yao

et al. 2023

Genes to Cells

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A simple method for producing pseudopregnant mice supports pup production. In this study, pregnant ICR were obtained mice without mating with vasectomized mice via administration of mouse Kisspeptin‐10 (mKp‐10) and transferring blastocysts to the uterus. Blastocyst transfer after mKp‐10 administration to mice with gapping and reddish pink vagina resulted in 65.2% (15/23) pregnancies, and 39.1% (34/87) of the transferred blastocysts showed full‐term growth. Vaginal smears were observed for accurate estrus cycle determination, and subsequent administration of mKp10 to mice during the estrus stage and blastocyst transfer resulted in 95.2% (20/21) pregnancies and 50.7% (104/205) birth rates. Regarding 2‐cell transfer after administration of mKp‐10, 100% (8/8) of the mice became pregnant, and 45.0% (36/80) of the embryos were born. Administration of mKp‐10 to mice during the estrus stage is a convenient way to generate pseudopregnant mice.

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Section: Discussionmentioning

confidence: 99%

Intraperitoneal administration of mouse kisspeptin‐10 to mice during estrus stage induces pseudopregnancy

Mashiko,

Tokoro,

Yao

et al. 2023

Genes to Cells

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“…Fertile and sexually active male rats were introduced on day three after administration of RICOM 1013-J into each group (ratio of 3 females to 1 male per cage) till the end of experiments for mating according to the methods of [10]. Mating was confirmed by the presence of a whitish vaginal plug with the presence of spermatozoa and is considered to be day '1' of conception [27,28]. The male rats were in contact with the female rats for the seven gestation periods of the study.…”

Section: Anti-conceptive Studies On Ricom 1013-jmentioning

confidence: 99%

Antifertility Efficacy of n-Hexane Seed Extract of Ricinus communis Var Minor in Wistar Rats Uterus In Vitro

Sanni,

Moritiwon,

Ogundeko

et al. 2024

PoS

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The seed of Ricinus communis var minor (RICOM 1013-J) is a popular contraceptive among the tribal women in Bassa Local Government Area of Plateau State, Nigeria. Several reports have confirmed the efficacy and safety of different fractions of RICOM 1013-J, particularly the n-Hexane fraction. RICOM 1013-J appears to possess a unique contraceptive effect. This study aimed to provide further insight into the impact of RICOM 1013-J on the reproductive organs compared with the activity profile of some uterotonic drugs. A total of 20 adult albino rats (15 females and five males) weighing 150-200 g were initially divided into five groups of 3 female rats each with treatments via the oral route as follows: Groups A (2ml olive oil), others n-Hexane extract of Ricinus communis seeds (RICOM 1013-J) B (5 mg/kg), C (10 mg/kg), D (20 mg/kg) and E (30 mg/kg). After three days, the five male rats were introduced into each group (ratio of 3 females to 1 male per cage) till the end of experiments for mating. The effect of the pretreatment with the n-hexane extract of RICOM 1013-J on the rat uterus was evaluated after days 10, 30, 60 and 90. RICOM 1013-J (20 mg/kg and 30 mg/kg) demonstrated potent anti-conceptive effects, protecting female Wister albino rats against pregnancy for over five gestational periods. RICOM 1013-J statistically altered the regular basal activity of the uterus in a time-dependent manner (10, 30, 60 and 90 days pretreatment). Furthermore, pretreatment with RICOM 1013-J decreased the reactivity of the uterus to some uterotonic drugs, including oxytocin (2x10-3 iu/ml), ergometrine (1x10 -3 mg/ml), misoprostol (2x10 -3 mg/ml), Ach (1x10 -5 gm/ml) and potassium chloride (1x10-3 gm/ml). The contraction to misoprostol and potassium chloride in the uterus was abolished on day 90 of pretreatment. This study has demonstrated the dose-dependent efficacy of RICOM 1013-J in protecting against pregnancy for over five gestation periods in rats. This protective effect may be due to the alterations in the activity profile of smooth muscle quiescence and inertia in the uterus. In addition, the changes in the responsiveness of the uterus to the uterotonic drugs further confirm the anti-conceptive effects of RICOM 1013-J.

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“…Then, 10 to 16-week-old C57BL/6 female mice purchased from BioLASCO Taiwan were used to induce pseudopregnancy and estrus synchronization was induced by administration of the luteinizing hormone releasing hormone agonist (LHRHa), as described in the previous study. 103 To efficiently synchronize the mating time for both pseudopregnant and superovulated female mice, the estrus synchronization was induced by the first i.p. injection of 20 μg of the LHRHa, namely [des-Gly 10 , D-Ala 6 ]-LH-RH ethylamide acetate salt hydrate (Sigma-Aldrich Product No.…”

Section: Pseudopregnancy Induction and Unilateral Embryo Transfers In...mentioning

confidence: 99%

Maternal exposure to hyperbaric oxygen at the preimplantation stages increases apoptosis and ectopic Cdx2 expression and decreases Oct4 expression in mouse blastocysts via Nrf2‐Notch1 upregulation and Nf2 downregulation

Li,

Chung,

et al. 2023

Developmental Dynamics

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BackgroundThe environmental oxygen tension has been reported to impact the blastocyst quality and cell numbers in the inner cell mass (ICM) during human and murine embryogenesis. While the molecular mechanisms leading to increased ICM cell numbers and pluripotency gene expression under hypoxia have been deciphered, it remains unknown which regulatory pathways caused the underweight fetal body and overweight placenta after maternal exposure to hyperbaric oxygen (HBO).ResultsThe blastocysts from the HBO‐exposed pregnant mice revealed significantly increased signals of reactive oxygen species (ROS) and nuclear Nrf2 staining, decreased Nf2 and Oct4 expression, increased nuclear Tp53bp1 and active caspase‐3 staining, and ectopic nuclear signals of Cdx2, Yap, and the Notch1 intracellular domain (N1ICD) in the ICM. In the ICM of the HBO‐exposed blastocysts, both Nf2 cDNA microinjection and Nrf2 shRNA microinjection significantly decreased the ectopic nuclear expression of Cdx2, Tp53bp1, and Yap whereas increased Oct4 expression, while Nrf2 shRNA microinjection also significantly decreased Notch1 mRNA levels and nuclear expression of N1ICD and active caspase‐3.ConclusionWe show for the first time that maternal exposure to HBO at the preimplantation stage induces apoptosis and impairs ICM cell specification via upregulating Nrf2‐Notch1‐Cdx2 expression and downregulating Nf2‐Oct4 expression.

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Scheduled simple production method of pseudopregnant female mice for embryo transfer using the luteinizing hormone-releasing hormone agonist

Cited by 3 publications

References 44 publications

Intraperitoneal administration of mouse kisspeptin‐10 to mice during estrus stage induces pseudopregnancy

Intraperitoneal administration of mouse kisspeptin‐10 to mice during estrus stage induces pseudopregnancy

Antifertility Efficacy of n-Hexane Seed Extract of Ricinus communis Var Minor in Wistar Rats Uterus In Vitro

Maternal exposure to hyperbaric oxygen at the preimplantation stages increases apoptosis and ectopic Cdx2 expression and decreases Oct4 expression in mouse blastocysts via Nrf2‐Notch1 upregulation and Nf2 downregulation

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