Schinus terebinthifolia leaf lectin (SteLL) is an immunomodulatory agent by altering cytokine release by mice splenocytes

Santos, Ardilles Juan Carlos Alves dos; Barros, Bárbara Rafaela da Silva; Aguiar, Lethícia Maria de Souza; Patriota, Leydianne Leite de Siqueira; Lima, Thâmarah de Albuquerque; Zingali, Russolina B.; Paiva, Patrícia Maria Guedes; Napoleão, Thiago Henrique; Melo, Cristiane Moutinho Lagos de; Pontual, Emmanuel Viana

doi:10.1007/s13205-020-2137-2

Cited by 14 publications

(6 citation statements)

References 26 publications

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“…This result agrees with previous studies that showed that ApuL and SteLL do not have cytotoxic effects on healthy cells. Brito et al [4] demonstrated the safety of ApuL with regards to human lymphocytes, while Santos et al [11] showed that SteLL (3.12-50 µg/mL) is not cytotoxic for mouse splenocytes. Additionally, the viability of macrophages isolated from mice was not affected by SteLL (2.0-16.0 µg/mL) [60].…”

Section: Discussionmentioning

confidence: 99%

“…An in vivo antiangiogenic property of SteLL was proved in the Coturnix japonica embryo model [10]. Furthermore, SteLL showed immunomodulatory effect on mouse splenocytes, stimulating the release of interleukins (IL-2, IL-4, and IL-17A), tumor necrosis factor alpha (TNF-α), interferon-gamma (IFN-γ), and reducing the secretion of nitric oxide (NO) [11]. Similar to ApuL, the hemagglutinating ability of SteLL was also not affected by monosaccharides (such as N-acetylglucosamine), but it was inhibited by the glycoproteins fetuin and ovalbumin [12].…”

Section: Introductionmentioning

confidence: 99%

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Effects of Lectins from Alpinia purpurata Inflorescence (ApuL) and Schinus terebinthifolia Leaf (SteLL) on Human Leukemic Cell Lines and Mesenchymal Stem Cells

et al. 2023

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Lectins (carbohydrate-binding proteins) are able to distinguish different patterns of glycosylation on cell surfaces. This study investigated the effects of lectins from Alpinia purpurata inflorescence (ApuL) and Schinus terebinthifolia leaf (SteLL) on the viability of human leukemia cells (K562, chronic myeloid leukemia; JURKAT, acute lymphoblastic leukemia) and mesenchymal stem cells (MSCs) from human umbilical cords. In addition, possible immunomodulatory effects of ApuL and SteLL on MSCs were assessed by determining cytokine levels in cultures. ApuL reduced the viability of JURKAT cells (IC50: 12.5 μg/mL), inducing both apoptosis and necrosis. For K562 cells, ApuL at 50 µg/mL caused a decrease in viability, but of only 8.8%. Conversely, SteLL exerted a cytotoxic effect on K562 (IC50: 6.0 μg/mL), inducing apoptosis, while it was not cytotoxic to JURKAT. ApuL and SteLL (0.19–100 μg/mL) did not decrease MSCs viability. Treatment with ApuL strongly suppressed (99.5% reduction) the release of IL-6 by MSCs. SteLL also reduced the levels of this cytokine in culture supernatant. In conclusion, ApuL and SteLL showed potential to reduce the viability of leukemia cells, as well as immunomodulatory effect on MSCs without being toxic to them. These biological properties can be explored biomedically and biotechnologically in the future.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Effects of Lectins from Alpinia purpurata Inflorescence (ApuL) and Schinus terebinthifolia Leaf (SteLL) on Human Leukemic Cell Lines and Mesenchymal Stem Cells

et al. 2023

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“…Plant lectins can induce the expression of humoral effectors against tumors or microbial infections [136,[141][142][143]. Activated macrophages can produce cytokine signal molecules.…”

Section: Plant Lectins Promote the Release Of Cytokines And Other Effectorsmentioning

confidence: 99%

“…The lectins stimulate cytokine production in different types of immune-related mammalian cells, such as murine macrophages, spleen cells, dendritic cells, mouse spleen cells, and human dendritic cells, neutrophils, peripheral blood mononuclear cells, etc. [136,142,143].…”

Section: Plant Lectins Promote the Release Of Cytokines And Other Effectorsmentioning

confidence: 99%

“…While the plant-lectin-induced insect immune responses are far from being clear, plant lectins can still be useful tools to investigate insect innate immunity. First of all, in mammalian systems, plant lectins have been analyzed in more detail for their immunomodulatory effects, especially regarding receptors on the cell membrane and in the cytosol, in both the cellular and humoral immune responses [136,[141][142][143]. In addition, the signaling pathways behind these responses have been extensively studied in vertebrate models [131].…”

Section: Plant Lectins As a Tool To Study Insect Immune Responsesmentioning

confidence: 99%

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Can Plant Lectins Help to Elucidate Insect Lectin-Mediated Immune Response?

Chen

Schutter

Damme

et al. 2021

Insects

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Lectins are carbohydrate-binding proteins that recognize and selectively bind to specific sugar structures. This group of proteins is widespread in plants, animals, and microorganisms, and exerts a broad range of functions. Many plant lectins were identified as exogenous stimuli of vertebrate immunity. Despite being the largest and most diverse taxon on earth, the study of lectins and their functions in insects is lagging behind. In insects, research on lectins and their biological importance has mainly focused on the C-type lectin (CTL) family, limiting our global understanding of the function of insect lectins and their role in insect immunity. In contrast, plant lectins have been well characterized and the immunomodulatory effects of several plant lectins have been documented extensively in vertebrates. This information could complement the missing knowledge on endogenous insect lectins and contribute to understanding of the processes and mechanisms by which lectins participate in insect immunity. This review summarizes existing studies of immune responses stimulated by endogenous or exogenous lectins. Understanding how lectins modulate insect immune responses can provide insight which, in turn, can help to elaborate novel ideas applicable for the protection of beneficial insects and the development of novel pest control strategies.

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