Schistosoma mansoni: Gene expression of the nucleotide excision repair factor 2 (NEF2) during the parasite life cycle, and in adult worms after exposure to different DNA-damaging agents

Silva, Camila S.; Silva, Sérgio H.; Pereira-Júnior, Olavo S.; Cabral, Fernanda Janku; Costa-Cruz, Júlia Maria; Rodrigues, Vanderlei

doi:10.1016/j.actatropica.2007.07.006

Cited by 7 publications

(4 citation statements)

References 43 publications

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“…Recent genetic and bioinformatics analyses confirm the presence of DNA repair machinery in this lower eukaryote (Silva et al 2007; Onyangoa et al 2011; Achanta et al 2012). However, little is known about DNA repair mechanisms and the proteins involved in apicomplexan parasites such as T. gondii .…”

Section: Dna Repair Mechanisms and Toxoplasma Gondii Infectionmentioning

confidence: 82%

“…Nucleotide excision repair system is an important repair pathway during the parasite Schistosoma mansoni life cycle (Silva et al 2007), but the role of NER in T. gondii life cycle has not yet been clarified. The DNA damage in intracellular parasites is great, but very little is known about the NER type of DNA repair machineries that exist in Toxoplasma .…”

Section: Dna Repair Mechanisms and Toxoplasma Gondii Infectionmentioning

confidence: 99%

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DNA repair mechanisms and Toxoplasma gondii infection

2013

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Lately, we can observe significant progress in understanding mechanism of DNA repair owing to fast methods of DNA sequence analysis from different organisms the revealing of structure and function of DNA repair proteins in prokaryota and eukaryota. The protozoan parasites survival depends on DNA repair systems. Better understanding of DNA repair systems can help in new antipathogen drug development. This review is aimed at updating our current knowledge of the various repair pathways by providing an overview of DNA repair genes regarding Toxoplasma gondii infections and the corresponding proteins, participating either directly in DNA repair, or in checkpoint control and signaling of DNA damage.

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Section: Dna Repair Mechanisms and Toxoplasma Gondii Infectionmentioning

confidence: 82%

Section: Dna Repair Mechanisms and Toxoplasma Gondii Infectionmentioning

confidence: 99%

DNA repair mechanisms and Toxoplasma gondii infection

2013

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“…mansoni , the RAD23 gene is expressed in all developmental stages of the parasite: egg, sporocysts, cercariae, schistosomula and adult worms. This gene showed differential expression in the presence of DNA-damaging agents [ 47 ]. In this study, PGM and RAD23 were successfully subcloned, expressed as soluble proteins in E .…”

Section: Discussionmentioning

confidence: 99%

Screening Diagnostic Candidates for Schistosomiasis from Tegument Proteins of Adult Schistosoma japonicum Using an Immunoproteomic Approach

Zhang

et al. 2015

PLoS Negl Trop Dis

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BackgroundSchistosomiasis is one of the world’s most prevalent zoonotic diseases and a serious worldwide public health problem. Since the tegument (TG) of Schistosoma japonicum is in direct contact with the host and induces a host immune response against infection, the identification of immune response target molecules in the schistosome TG is crucial for screening diagnostic antigens for this disease.Methodology/Principal FindingsIn this study, an immunoproteomics approach used TG proteins as screening antigens to identify potential diagnostic molecules of S. japonicum. Ten spots corresponding to six proteins were identified that immunoreacted with sera from S. japonicum-infected rabbits but not sera from uninfected rabbits and their specific IgG antibody levels declined quickly after praziquantel treatment. Recombinant phosphoglycerate mutase (PGM) and UV excision repair protein RAD23 homolog B (RAD23) proteins were expressed and their diagnostic potential for schistosomiasis was evaluated and compared with schistosome soluble egg antigen (SEA) using ELISA. The results showed high sensitivity and specificity and low crossreactivity when rSjPGM-ELISA and rSjRAD23-ELISA were used to detect water buffalo schistosomiasis. Moreover, antibodies to rSjPGM and rSjRAD23 might be short-lived since they declined quickly after chemotherapy.Conclusion/SignificanceTherefore, the two schistosome TG proteins SjPGM and SjRAD23 were identified as potential diagnostic markers for the disease. The two recombinant proteins might have the potential to evaluate the effectiveness of drug treatments and for distinguishing between current and past infection.

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“…A c c e p t e d M a n u s c r i p t 7 Schistosoma mansoni, a water-borne parasite of humans with an intermediate molluscan host, has a complex life cycle in which it can be exposed to a subset of DNAdamaging agents, such as those that are present in the environment or the ones from the host immune responses. Using the comet assay, it was shown that DNA from adult worms can be damaged by different DNA-damaging agents such as tetramethylammonium chloride (TMA) and hydrogen peroxide [64].…”

Section: Platyhelminthes (Platodes)mentioning

confidence: 99%

The comet assay in animal models: From bugs to whales – (Part 1 Invertebrates)

Gajski

Žegura

Ladeira

et al. 2019

Mutation Research/Reviews in Mutation Research

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The comet assay, also called single cell gel electrophoresis, is a sensitive, rapid and low-cost technique for quantifying and analysing DNA damage and repair at the level of individual cells. The assay itself can be applied on virtually any cell type derived from different organs and tissues of eukaryotic organisms. Although it is mainly used on human cells, the assay has applications also in the evaluation of DNA damage in yeast, plant and animal cells. Therefore, the purpose of this review is to give an extensive overview on the usage of the comet assay in animal models from invertebrates to vertebrates, covering both terrestrial and water biota. The comet assay is used in a variety of invertebrate species since they are regarded as interesting subjects in ecotoxicological research due to their significance in ecosystems. Hence, the first part of the review (Part 1) will discuss the application of the comet assay in invertebrates covering protozoans, platyhelminthes, planarians, cnidarians, molluscs, annelids, arthropods and echinoderms. Besides a large number of animal species, the assay is also performed on a variety of cells, which includes haemolymph, gills, digestive gland, sperm and embryo cells. The mentioned cells have been used for the evaluation of a broad spectrum of genotoxic agents both in vitro and in vivo. Moreover, the use of invertebrate models and their role from an ecotoxicological point of view will also be discussed as well as the comparison of the use of the comet assay in invertebrate and human models. Since the comet assay is still developing, its increasing potential in assessing DNA damage in animal models is crucial especially in the field of ecotoxicology and biomonitoring at the level of different species, not only humans.

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Schistosoma mansoni: Gene expression of the nucleotide excision repair factor 2 (NEF2) during the parasite life cycle, and in adult worms after exposure to different DNA-damaging agents

Cited by 7 publications

References 43 publications

DNA repair mechanisms and Toxoplasma gondii infection

DNA repair mechanisms and Toxoplasma gondii infection

Screening Diagnostic Candidates for Schistosomiasis from Tegument Proteins of Adult Schistosoma japonicum Using an Immunoproteomic Approach

The comet assay in animal models: From bugs to whales – (Part 1 Invertebrates)

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