Schistosoma mansoni: In VitroAdhesion of Parasite Eggs to the Vascular Endothelium. Subsequent Inhibition by a Monoclonal Antibody Directed to a Carbohydrate Epitope

Lejoly-Boisseau, Hélène; Appriou, M.; Seigneur, M.; Pruvost, Annie; Tribouley-Duret, J; Tribouley, J

doi:10.1006/expr.1999.4348

Cited by 36 publications

(24 citation statements)

References 29 publications

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“…Human endothelial cell line ECV-304, HUVECs and the undifferentiated hESCs were cultured as described previously (Lejoly-Boisseau et al, 1999;Zhang et al, 2008).…”

Section: Culturing Ecv-304 Cells Huvecs and Hescsmentioning

confidence: 99%

The role of Hath6, a novel shear stress-responsive transcription factor, in endothelial differentiation and function modulation

Fang¹,

Wasserman²,

Torres-Vázquez³

et al. 2014

Journal of Cell Science

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The key regulators of endothelial differentiation that is induced by shear stress are mostly unclear. Human atonal homolog 6 (Hath6 or ATOH8) is an endothelial-selective and shear-stress-responsive transcription factor. In this study, we sought to elucidate the role of Hath6 in the endothelial specification of embryonic stem cells. In a stepwise human embryonic stem cell to endothelial cell (hESC-EC) induction system, Hath6 mRNA was upregulated synchronously with endothelial determination. Subsequently, gain-of-function and lossof-function studies of Hath6 were performed using the hESC-EC induction model and endothelial cell lines. The overexpression of Hath6, which mimics shear stress treatment, resulted in an increased CD45 2 CD31 + KDR + population, a higher tubular-structure-formation capacity and increased endothelial-specific gene expression. By contrast, the knockdown of Hath6 mRNA markedly decreased endothelial differentiation. Hath6 also facilitated the maturation of endothelial cells in terms of endothelial gene expression, tubularstructure formation and cell migration. We further demonstrated that the gene encoding eNOS is a direct target of Hath6 through a reporter system assay and western blot analysis, and that the inhibition of eNOS diminishes hESC-EC differentiation. These results suggest that eNOS plays a key role in linking Hath6 to the endothelial phenotype. Further in situ hybridization studies in zebrafish and mouse embryos indicated that homologs of Hath6 are involved in vasculogenesis and angiogenesis. This study provides the first confirmation of the positive impact of Hath6 on human embryonic endothelial differentiation and function. Moreover, we present a potential signaling pathway through which shear stress stimulates endothelial differentiation.

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“…Human endothelial cell line ECV-304, HUVECs and the undifferentiated hESCs were cultured as described previously (Lejoly-Boisseau et al, 1999;Zhang et al, 2008).…”

Section: Culturing Ecv-304 Cells Huvecs and Hescsmentioning

confidence: 99%

The role of Hath6, a novel shear stress-responsive transcription factor, in endothelial differentiation and function modulation

Fang¹,

Wasserman²,

Torres-Vázquez³

et al. 2014

Journal of Cell Science

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“…mansoni modulates its host's immune system to survive. In the course of schistosomiasis, worms, eggs and their soluble antigens, i.e., a complex mixture of proteins, glycoproteins and glycolipids, interact with vascular endothelium [49] and myocytes causing morphological alterations [4,8,34,35,[50][51][52]. Previous data showed that schistosomiasis is associated with a reduction of mesenteric endothelial eNOS expression and ATP-induced NO production [15].…”

Section: Discussionmentioning

confidence: 99%

Endothelial P2X7 receptors’ expression is reduced by schistosomiasis

Oliveira

Coutinho‐Silva

Silva

2012

Purinergic Signalling

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Endothelial cells control vascular tone, permeability and leukocyte transmigration and are modulated by pro-inflammatory mediators. Schistosomiasis is an intravascular disease associated with inflammation, therefore altering endothelial cells' phenotype. Purinergic P2X7 receptors (P2X7R) play an important role in inflammation; however, the impact of the disease upon endothelial P2X7R function or expression has not been explored. Using ethidium bromide uptake to investigate P2X7R function, we observed that the effects of ATP (3 mM) and the P2X7R agonist 3′-O-(4-benzoyl)-ATP (BzATP) were smaller in mesenteric endothelial cells from the Schistosoma mansoni-infected group than in the control group. In the control group, BzATP induced endothelial nitric oxide production, which was blocked by the P2X7R antagonists KN-62 and A740003. However, in the infected group, we observed a reduced effect of BzATP and no effect of both P2X7R antagonists, suggesting a downregulation of endothelial P2X7R in schistosomiasis. We observed similar results in both infected and P2X7R −/− groups, which were also comparable to data obtained with KN-62-or A740004-treated control cells. Data from Western blot and immunocytochemistry assays confirmed the reduced expression of P2X7R in the infected group. In conclusion, our data show a downregulation of P2X7R in schistosomiasis infection, which likely limits the infection-related endothelial damage.

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“…At this time, granuloma formation usually starts around them. Moreover, S. mansoni ova can interact with endothelial cells and provoke an inflammatory reaction [10,11] leading to vascular damage, such as necrotizing vasculitis [12]. Thus, the pathophysiology of vasculitis in the latter stages of neuroschistosomiasis (after onset of egg laying) can stem from the ability of eggs to interact with endothelium and provoke an inflammatory reaction in the vascular wall, decreasing the vessel lumen and thrombosing.…”

Section: Discussionmentioning

confidence: 99%

Cerebral vasculitis associated with Schistosoma mansoniinfection

et al. 2012

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BackgroundCerebral involvement in schistosomiasis is not rare, but it is underdiagnosed because of the lack of clinical suspicion and the frequency of asymptomatic forms. Neurologic complications are generally supported by granuloma formation around ectopic eggs which have migrated to the brain. Moreover, vascular lesions and cerebral arteritis have been well documented in histopathological studies. Nevertheless, cerebral vasculitis in later stages of the Schistosoma mansoni infection have not yet been described in living subjects.Case presentationA 28-year-old french woman had a stroke linked with cerebral vasculitis, 6 monthes after returning from Burkina-Faso. At the same time, a S. mansoni disseminated infection was diagnosed. She suffered from a new stroke after undertaking praziquantel therapy, which lead us to associate the S. mansoni infection and cerebral vasculitis.ConclusionThis is the first report of such association, since cerebral vasculitis has never been described in later stages of the S. mansoni infection. Although the causal link between the two pathologies could not be proved, we suggest that S. mansoni is able to cause severe vascular damage in cerebral vessels. Schistosomiasis must be investigated in the event of a brain infarct in young people, particularly in patients originating or returning from an endemic area.

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Schistosoma mansoni: In VitroAdhesion of Parasite Eggs to the Vascular Endothelium. Subsequent Inhibition by a Monoclonal Antibody Directed to a Carbohydrate Epitope

Cited by 36 publications

References 29 publications

The role of Hath6, a novel shear stress-responsive transcription factor, in endothelial differentiation and function modulation

The role of Hath6, a novel shear stress-responsive transcription factor, in endothelial differentiation and function modulation

Endothelial P2X7 receptors’ expression is reduced by schistosomiasis

Cerebral vasculitis associated with Schistosoma mansoniinfection

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