Schistosoma mansoni Infection but Not Egg Antigen Promotes Recovery from Colitis in Outbred NMRI Mice

Bodammer, Peggy; Waitz, Grit; Loebermann, Micha; Holtfreter, Martha C.; Maletzki, Claudia; Krueger, Marzena Renata; Nizze, H; Emmrich, Joerg; Reisinger, Emil C.

doi:10.1007/s10620-010-1237-y

Cited by 31 publications

(33 citation statements)

References 24 publications

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“…Schistosome egg induction of anti-inflammatory responses is essential in reducing hepatic inflammation and is key for host survival (22)(23)(24)(25)(26)(27). Similar to schistosome eggs, the saline homogenate of schistosome eggs, soluble egg antigen (SEA), also induces strong CD4 ϩ Th2 responses (15,22,23,(28)(29)(30)(31). In this regard, coadministration of SEA with a third-party protein antigen led to an increase in vaccine-specific Th2-type responses (32).…”

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confidence: 99%

Schistosoma mansoni Soluble Egg Antigens Enhance Listeria monocytogenes Vector HIV-1 Vaccine Induction of Cytotoxic T Cells

Bui

Shollenberger

Paterson

et al. 2014

Clin. Vaccine Immunol.

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Vaccines are an important public health measure for prevention and treatment of diseases. In addition to the vaccine immunogen, many vaccines incorporate adjuvants to stimulate the recipient's immune system and enhance vaccine-specific responses. While vaccine development has advanced from attenuated organism to recombinant protein or use of plasmid DNA, the development of new adjuvants that safely increase immune responses has not kept pace. Previous studies have shown that the complex mixture of molecules that comprise saline soluble egg antigens (SEA) from Schistosoma mansoni eggs functions to promote CD4 ؉ T helper 2 (Th2) responses. Therefore, we hypothesized that coadministration of SEA with a Listeria vector HIV-1 Gag (Lm-Gag) vaccine would suppress host cytotoxic T lymphocyte (CTL) and T helper 1 (Th1) responses to HIV-1 Gag epitopes. Surprisingly, instead of driving HIV-1 Gag-specific responses toward Th2 type, we found that coadministration of SEA with LmGag vaccine significantly increased the frequency of gamma interferon (IFN-␥)-producing Gag-specific Th1 and CTL responses over that seen in mice administered Lm-Gag only. Analysis of the functionality and durability of vaccine responses suggested that SEA not only enlarged different memory T cell compartments but induced functional and long-lasting vaccine-specific responses as well. These results suggest there are components in SEA that can synergize with potent inducers of strong and durable Th1-type responses such as those to Listeria. We hypothesize that SEA contains moieties that, if defined, can be used to expand type 1 proinflammatory responses for use in vaccines.

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confidence: 99%

Schistosoma mansoni Soluble Egg Antigens Enhance Listeria monocytogenes Vector HIV-1 Vaccine Induction of Cytotoxic T Cells

Bui

Shollenberger

Paterson

et al. 2014

Clin. Vaccine Immunol.

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“…The majority have shown therapeutic effects on experimental autoimmunity or allergy. Some investigators reported that viable parasites were superior to administration of the antigens of parasites (Hunter et al, 2010;Bodammer et al, 2011;Osada et al, 2010) in therapeutic efficacy. In addition, there is still considerable controversy over the roles of regulatory cells (e.g.…”

Section: Discussionmentioning

confidence: 99%

“…Schistosoma spp.) have anti-colitic effects (Moreels et al, 2004;Ruyssers et al, 2009;Elliott et al, 2003;Mo et al, 2007;Zhao et al, 2009;Bodammer et al, 2011;Smith et al, 2007). A down-regulation of Th1/Th17-related cytokines and upregulation of anti-inflammatory cytokines are observed in most studies.…”

Section: Experimental Colitis As a Model Of Ibdmentioning

confidence: 99%

Parasitic Helminths as Potential Therapeutic Agents Against Autoimmune Disorders

Osada¹

2011

Autoimmune Disorders - Current Concepts and Advances From Bedside to Mechanistic Insights

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“…One method to overcome helminth-induced vaccine suppression is to identify vaccine vectors that are capable of producing vaccine-specific responses irrespective of helminth infection. In this regard, a Listeria vector HIV vaccine was recently shown to induce potent CD8 ϩ and Th1-type vaccine responses in schistosome-infected mice (12, 13).Schistosome soluble egg antigens (SEA) are potent inducers of CD4 ϩ Th2-type biasing (4,6,9,21), and in a previous study, we demonstrated that SEA coadministered with a third-party antigen was sufficient to induce vaccine-specific Th2-type cytokine responses (9). Here, we evaluated the influence of SEA on the ability of a Listeria vector HIV-1 vaccine to drive Th1-type and CTL responses in Th1-type C57BL/6 mice.…”

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confidence: 99%

“…Schistosome soluble egg antigens (SEA) are potent inducers of CD4 ϩ Th2-type biasing (4,6,9,21), and in a previous study, we demonstrated that SEA coadministered with a third-party antigen was sufficient to induce vaccine-specific Th2-type cytokine responses (9). Here, we evaluated the influence of SEA on the ability of a Listeria vector HIV-1 vaccine to drive Th1-type and CTL responses in Th1-type C57BL/6 mice.…”

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confidence: 99%

Schistosoma mansoni Soluble Egg Antigens Enhance T Cell Responses to a Newly Identified HIV-1 Gag H-2^bEpitope

Bui

Shollenberger

Paterson

et al. 2014

Clin. Vaccine Immunol.

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c Schistosome infection induces significant T helper type 2 (Th2) and anti-inflammatory immune responses and has been shown to negatively impact vaccine efficacy. Our goal was to determine if the administration of schistosome soluble egg antigens (SEA) would negatively influence the induction of cytotoxic T lymphocyte (CTL) and Th1-type T cell responses to an HIV candidate vaccine in the Th1-biased C57BL/6 mouse strain. Initial experiments failed, as we were unable to detect any response to the defined class I epitope for HIV-1 IIIB Gag. Therefore, we initiated an epitope mapping study to identify C57BL/6 (H-2 b ) T cell epitopes in HIV-1 IIIB Gag in order to perform the experiments. This analysis defined two previously unreported minimal class I H-2 b and class II I-A b epitopes for HIV-1 IIIB Gag. The newly defined HIV-1 IIIB Gag epitopes were used to evaluate the influence of SEA on the generation of CTL and Th1-type HIV-1 IIIB Gag responses. Surprisingly, in contrast to our hypothesis, we observed that the coadministration of SEA with a Listeria monocytogenes vector expressing HIV-1 IIIB Gag (Lm-Gag) led to a significantly increased frequency of gamma interferon (IFN-␥)-producing CD8؉ and CD4 ؉ T cells in C57BL/6 mice compared to mice immunized with Lm-Gag only. These observations suggest that SEA contains, in addition to Th2-type and immune-suppressive molecules, substances that can act with the Lm-Gag vaccine to increase CTL and Th1-type vaccine-specific immune responses.V accines for human immunodeficiency virus (HIV), tuberculosis, and malaria are in development or in clinical trials. The greatest incidence of each of these diseases is in sub-Saharan Africa, where helminth infection is endemic (1, 2). Helminth parasites bias immune responses to the CD4 ϩ Th2 type and can be immune suppressive (3-10). To evaluate the influence of helminth infection on vaccines, we can evaluate the ability to induce vaccine-specific immune responses in helminth-infected recipients or in recipients who have been treated with immune-biasing helminth antigens (11-13).Schistosomiasis is a helminth parasitic disease that affects Ͼ200 million people worldwide and is listed by the World Health Organization (WHO) as the second leading parasitic disease, after malaria (14). Infection occurs when cercariae that emerge from infected snail intermediate hosts contact and penetrate the skin of the vertebrate host. Larval parasites migrate and mature into adult male and female worms that mate and produce eggs (4, 6). Eggs that become lodged within host tissues are largely responsible for Th2 biasing of the host immune system and induction of antiinflammatory responses (3,4,6,15,16).Immune biasing induced by schistosome infection has been shown to reduce vaccine efficacy in both laboratory and clinical settings (8,11,17,18). Specifically, helminth infection has been shown to suppress immune responses to a Th1-type vaccine and impair the expansion of pathogen-specific cytotoxic CD8ϩ T cell (cytotoxic T lymphocyte [CTL]) responses (18)(19)(...

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Schistosoma mansoni Infection but Not Egg Antigen Promotes Recovery from Colitis in Outbred NMRI Mice

Abstract: The results presented here suggest that SEA treatment could not protect mice from acute colitis. However, both infection with S. mansoni and injection of SEA affect mucosal immune responses.

Cited by 31 publications

References 24 publications

Schistosoma mansoni Soluble Egg Antigens Enhance Listeria monocytogenes Vector HIV-1 Vaccine Induction of Cytotoxic T Cells

Schistosoma mansoni Soluble Egg Antigens Enhance Listeria monocytogenes Vector HIV-1 Vaccine Induction of Cytotoxic T Cells

Parasitic Helminths as Potential Therapeutic Agents Against Autoimmune Disorders

Schistosoma mansoni Soluble Egg Antigens Enhance T Cell Responses to a Newly Identified HIV-1 Gag H-2^bEpitope

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Schistosoma mansoni Infection but Not Egg Antigen Promotes Recovery from Colitis in Outbred NMRI Mice

Abstract: The results presented here suggest that SEA treatment could not protect mice from acute colitis. However, both infection with S. mansoni and injection of SEA affect mucosal immune responses.

Cited by 31 publications

References 24 publications

Schistosoma mansoni Soluble Egg Antigens Enhance Listeria monocytogenes Vector HIV-1 Vaccine Induction of Cytotoxic T Cells

Schistosoma mansoni Soluble Egg Antigens Enhance Listeria monocytogenes Vector HIV-1 Vaccine Induction of Cytotoxic T Cells

Parasitic Helminths as Potential Therapeutic Agents Against Autoimmune Disorders

Schistosoma mansoni Soluble Egg Antigens Enhance T Cell Responses to a Newly Identified HIV-1 Gag H-2bEpitope

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Schistosoma mansoni Soluble Egg Antigens Enhance T Cell Responses to a Newly Identified HIV-1 Gag H-2^bEpitope