Schistosoma mansoni:Susceptibility Differences between Male and Female Mice Can Be Mediated by Testosterone during Early Infection

Nakazawa, Makoto; Fantappíé, Marcelo Rosado; Freeman, George L.; Elói-Santos, Silvana Maria; Olsen, Nancy J.; Kovacs, William J.; Secor, W. Evan; Colley, Daniel G.

doi:10.1006/expr.1997.4148

Cited by 73 publications

(64 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It is worth emphasizing that all groups of animals were infected with the same cercaria suspension by the same person in a random order and with no delay between groups, so that differences in cercarial virulence or viability could not account for the latter findings. Besides, worm burdens were investigated in groups of female wild-type and CCL3-deficient mice, excluding the possibility that sex-specific susceptibilities to infection could have biased the results (16,33).…”

Section: Discussionmentioning

confidence: 99%

Potential Role of the Chemokine Macrophage Inflammatory Protein 1α in Human and Experimental Schistosomiasis

Souza¹,

Roffê²,

Pinho³

et al. 2005

Infect Immun

View full text Add to dashboard Cite

In human schistosomiasis, the concentrations of the chemokine macrophage inflammatory protein 1␣ (MIP-1␣/CCL3) is greater in the plasma of patients with clinical hepatosplenic disease. The objective of the present study was to confirm the ability of CCL3 to detect severe disease in patients classified by ultrasonography (US) and to evaluate the potential role of CCL3 in Schistosoma mansoni-infected mice. CCL3 was measured by enzyme-linked immunosorbent assay in the plasma of S. mansoni-infected patients. CCL3-deficient mice were infected with 25 cercariae, and various inflammatory and infectious indices were evaluated. The concentration of CCL3 was higher in the plasma of S. mansoni-infected than noninfected patients. Moreover, CCL3 was greater in those with US-defined hepatosplenic than with the intestinal form of the disease. In CCL3-deficient mice, the size of the granuloma and the liver eosinophil peroxidase activity and collagen content were diminished compared to wild-type mice. In CCL3-deficient mice, the worm burden after 14 weeks of infection, but not after 9 weeks, was consistently smaller. The in vitro response of mesenteric lymph node cells to antigen stimulation was characterized by lower levels of interleukin-4 (IL-4) and IL-10. CCL3 is a marker of disease severity in infected humans, and experimental studies in mice suggest that CCL3 may be a causative factor in the development of severe schistosomiasis.Schistosomiasis is one of the most prevalent helminth diseases in the world and is caused by blood flukes of the Schistosoma genus (36). In infected individuals, the granulomatous inflammation in response to egg deposition in the liver in the case of Schistosoma mansoni is the major pathological finding and accounts for most of the clinical symptoms. Worm oviposition at 5 to 6 weeks poses a strong Th2 bias in the immune response against infection (6) while also inhibiting the Th1 component (12,42). Nonetheless, the granulomatous response that is maximal during the first few weeks after initial oviposition is also subjected to immunomodulation. Apart from interleukin-10 (IL-10) (29), other factors may also be involved in this process. For example, recent literature suggests that, in animal models, the granulomatous response that occurs at chronic time points is dependent on the soluble form of the IL-13 receptor ␣2 (27). In patients, failure in modulating the response might lead to the development severe schistosomiasis later in life (15). Indeed, a direct consequence of the persistence of an exacerbated immune response appears to be the development of large granulomatous reactions associated with intense collagen deposition (20) and the development of hepatosplenic schistosomiasis. Therefore, there is much scientific interest in the understanding of the mechanisms and inflammatory mediators underlying egg-induced granulomatous response, with the ultimate goal of proposing strategies to modulate fibrosis. It is known, however, that prevention of granuloma formation may be dangerous, since lethality ...

show abstract

Section: Discussionmentioning

confidence: 99%

Potential Role of the Chemokine Macrophage Inflammatory Protein 1α in Human and Experimental Schistosomiasis

Souza¹,

Roffê²,

Pinho³

et al. 2005

Infect Immun

View full text Add to dashboard Cite

show abstract

“…Fewer worms develop in male rodent hosts than in females and castration and exogenous hormones have the expected effects (Berg 1957, Nakazawa et al 1997. Fulford et al (1998) propose that in mice and in humans that this might be related to dehydroepiandrosterone levels.…”

Section: Worm Development Fecundity and Fecal Egg Excretionmentioning

confidence: 99%

Experimental models of Schistosoma mansoni infection

Cheever

Lenzi²,

Lenzi³

et al. 2002

Mem. Inst. Oswaldo Cruz

123

View full text Add to dashboard Cite

“…In the present study, we have conducted our experiment on female mice as it has been revealed that schistosomiasis is an immune-related disease and female mice can express prominent levels of immune response due to estrogen hormone. On the contrary, androgen of male has negative effect on immune response [19,20] . In the present work, light microscopic examination of sections of subgroup BI displayed eggs deposition in the splenic parenchyma which was disrupted.…”

Section: Discussionmentioning

confidence: 99%

Histological study on the possible therapeutic role of bone marrow derived mesenchymal stem cells in a model of Schistosoma mansoni infestation of spleen of mice

Raafat

Gawad

Fikry

2017

Egyptian Journal of Histology

View full text Add to dashboard Cite

Background: Hepatosplenomegaly is a characteristic feature of Schistosoma infestation. However, splenic injury had received little scientific researches than the well-known liver injury. Moreover, the role of bone marrow derived mesenchymal stem cells (BMMSCs) in treatment of splenic injury due to schistosomiasis has not yet been investigated. Aim of the work: To explore the structural changes which might occur to spleen during chronic infestation with schistosomiasis and the possible therapeutic role of (BMMSCs) in ameliorating these changes. Materials & Methods: Fifty female Swiss Albino mice, weighing about 25 gm were classified into group A (control group) and group B (experimental group). Animals in group A were equally subdivided into subgroup AI which served as donors for stem cells obtained from their bone marrow, and subgroup AII which were injected with phosphate buffer saline (PBS) and used to collect control spleen samples. Whereas, animals in group B, were all infected with S. mansoni cercariae (60/ mouse) by subcutaneous injection, then subdivided into three subgroups; subgroup BI sacrificed after eight weeks, subgroup BII treated intraperitoneally with 2x106 MSCs suspended in PBS per mouse at eighth week after infestation hen scarified four weeks later, and subgroup BIII allowed to survive for twelve weeks without treatment then sacrificed. Results: Histological examination of spleen sections of subgroup BI showed structural changes including deposition of eggs which were surrounded by inflammatory cells and collagen fibers. Subgroup BIII showed more extensive structural changes. This was associated with significant increase in collagen fibers and TNF-α immunological reaction compared to control. However, (BMMSCs) treated subgroup BII illustrated improvement of splenic structure. Conclusions: Chronic Schistosoma mansoni infestation has a deleterious effect on the structure of the spleen. Bone marrow derived mesenchymal stem cells have a relevant therapeutic potential on the spleen of an animal model of Schistosoma mansoni.

show abstract

Schistosoma mansoni:Susceptibility Differences between Male and Female Mice Can Be Mediated by Testosterone during Early Infection

Cited by 73 publications

References 14 publications

Potential Role of the Chemokine Macrophage Inflammatory Protein 1α in Human and Experimental Schistosomiasis

Potential Role of the Chemokine Macrophage Inflammatory Protein 1α in Human and Experimental Schistosomiasis

Experimental models of Schistosoma mansoni infection

Histological study on the possible therapeutic role of bone marrow derived mesenchymal stem cells in a model of Schistosoma mansoni infestation of spleen of mice

Contact Info

Product

Resources

About