Schizochytrium limacinum altered antioxidant capacity and transcriptome profiles in Pacific white shrimp fed a low-fishmeal diet

Xu, Jia; Yao, Xinzhou; Lin, Yingying; Chi, Shuyan; Zhang, Shuang; Cao, Jiankang; Tan, Beiping; Xie, Shiwei

doi:10.1016/j.aqrep.2022.101399

Cited by 3 publications

(4 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Additionally, the substitution of fish meal with BSFL meal in the diet resulted in a lower TC and TG concentration in juvenile striped catfish [ 35 ], which could be responsible for the presence of chitin in BSFL, which plays an important role in triglyceride hydrolysis and in boosting lipid utilization [ 36 ]. The low-level dietary fishmeal supplemented with 0.3% and 0.6% schizochytrium also significantly reduced the content of TG and TC [ 37 ] and had the potential to increase the CA concentration in plasma [ 16 ]. Levels of enzyme activities, such as AST and ALT, and contents of CREA and BUN are related to the health of the liver and kidney.…”

Section: Discussionmentioning

confidence: 99%

The Effect of Dietary Protein Hydrolysate from Black Soldier Fly Larvae and Schizochytrium on Palatability, Nutrient Metabolites and Health Status in Beagle Dogs

Wei,

Xue,

et al. 2024

Metabolites

View full text Add to dashboard Cite

Protein hydrolysate from black soldier fly larvae (BSFP) has garnered great attention with its lower allergenicity, high amount of essential amino acids, and small bioactive peptides. Schizochytrium is a promising alternative source of n-3 FUFA because it has enriched docosahexaenoic acid (DHA, C22: 6). The aim of this study was to assess palatability, the presence of diarrhea, plasma biochemistry panels, anti-oxidative and anti-inflammatory effects, and immune function in beagle dogs when supplementing a mixture of protein hydrolysate from black soldier fly larvae and schizochytrium (BSFPs) into their diets. Experiment I: 24 young beagle dogs (16 males and 8 females; 4–5 months; BW: 6.40 ± 0.15 kg) were randomly divided into four groups: (1) control (CON), (2) 5% BSFPs, (3) 10% BSFPs, (4) 15% BSFPs. Their body weights and fecal scores were recorded, and blood samples were collected for analysis. Experiment II: three diets containing 5%, 10%, and 15% BSFPs were evaluated by comparing them with a basal diet (CON) to evaluate palatability. These results suggested that a lower presence of diarrhea existed in the BSFP diet than the CON diet (p < 0.05). Three treatment groups remarkably increased their total protein (TP) and albumin (ALB) contents and decreased their concentrations of triglyceride (TG) and total cholesterol (TC) in plasma (p < 0.05). Moreover, the 5% and 15% BSFPs groups had a higher calcium (CA) content in plasma, and the activities of aspartate aminotransferase (AST) and alanine aminotransferase (ALT) and contents of creatinine (CREA) and urea nitrogen (BUN) were significantly reduced by supplementing BSFP in their diets (p < 0.05). Their anti-oxidative enzyme activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-PX) were dramatically enhanced, and their malondialdehyde (MDA) concentrations were remarkably reduced (p < 0.05). Immunoglobulin A and G (IgA and IgG) concentrations in the plasma in the 10% and 15% BSFPs groups were significantly increased (p < 0.05). Furthermore, lower interleukin-8 (IL-8) contents were shown in the BSFP diets than the CON diet (p < 0.05). Similarly, the diets supplemented with BSFPs exhibited a positive effect on palatability (p < 0.05). To sum up, the diets supplemented with BSFPs significantly enhanced palatability, immune function, and anti-oxidative and anti-inflammatory capacity to alleviate diarrhea and improve the general health of the beagle dogs.

show abstract

Section: Discussionmentioning

confidence: 99%

The Effect of Dietary Protein Hydrolysate from Black Soldier Fly Larvae and Schizochytrium on Palatability, Nutrient Metabolites and Health Status in Beagle Dogs

Wei,

Xue,

et al. 2024

Metabolites

View full text Add to dashboard Cite

show abstract

“…Secondly, supplementation of thraustochytrid biomass can enhance the antioxidant capacity of shrimps. Low-fishmeal diets supplemented with S. limacinum significantly improved the antioxidant capacity of Litopenaeus vannamei, altering its transcriptomic expression patterns, particularly genes related to antioxidant and immune functions, thus enhancing the health status of the shrimp (Xu et al, 2022). Moreover, the biofloc technology is an emerging and environmentally friendly aquaculture method.…”

Section: The Role Of Thraustochytrid Supplements In Growth Health And...mentioning

confidence: 99%

“…Several extraction methods of lipids containing high levels of DHA were established including enzymatic ethanolysis (He et al, 2019), high shear-assisted solvent extraction (Kwak et al, 2019), supercritical CO 2 extraction (de Melo et al, 2020) and ultrasound-assisted extraction (Li et al, 2021). Remarkably, plenty of recent researches demonstrated that feeding supplementation of biomass or extracts of thraustochytrids was beneficial for aquatic animals such as Nile tilapia (dos Santos et al, 2019), golden pompano (Xie et al, 2019), Pacific white shrimp (Xu et al, 2022) and Penaeus monodon larvae (Visudtiphole et al, 2021), as well as Japanese quail (Gladkowski et al, 2014), layer hen (Moran et al, 2020) and steers (Carvalho et al, 2018) in improving their growth performance, survival, immunity, anti-inflammatory effect, and regulating the blood cells and gut microbiota (Souza et al, 2020). Presumably, thraustochytrids will become an important source of DHA in animal originated foods in our daily diet, and thus improve human health (Figure 1).…”

Section: Introductionmentioning

confidence: 99%

Marine thraustochytrid: exploration from taxonomic challenges to biotechnological applications

Wang,

Zhang,

Hui

et al. 2024

Front. Mar. Sci.

View full text Add to dashboard Cite

Thraustochytrids, as a distinct group of heterotrophic protists, have garnered considerable attention owing to their remarkable adaptability in extreme marine environments, pronounced capacity for metabolic regulation and prolific production of high-value metabolites. The taxonomic classification of these microorganisms presents a substantial challenge due to the variability in morphological characteristics under different culture conditions. And this undermines the efficacy of traditional classification systems on physiological and biochemical traits. The establishment of a polyphasic taxonomic system integrating genomic characteristics in the future will provide new avenues for more accurate classification and identification. Thraustochytrids can effectively accumulate bioactive substances such as docosahexaenoic acid (DHA), eicosapentaenoic acid (EPA), squalene and carotenoids. Through fermentation optimization and genetic modification, scientists have significantly enhanced the production of these metabolites. Moreover, the application of thraustochytrids in aquaculture, poultry and livestock feed has significantly improved animal growth and physiological indicators meanwhile increasing their DHA content. Natural bioactive substances in thraustochytrids, such as terpenoid compounds with antioxidant properties, have been proposed for application in the cosmetics industry. In the field of pharmacology, thraustochytrids have shown certain anti-inflammatory and anti-cancer activities and provide potential for the development of new oral vaccines. Additionally, they can degrade various industrial and agricultural wastes for growth and fatty acid production, demonstrating their potential in environmental bioremediation. Therefore, thraustochytrids not only exhibit tremendous application potential in the field of biotechnology, but also hold significant value in environmental protection and commercialization.

show abstract

“…In another study, the authors identified that differentially expressed genes (DEGs) related to detoxification were associated with the intestinal microbiota, which sheds light on the competitive dynamics among specific bacteria [24]. Jia et al utilized transcriptomic analysis to probe into the adverse effects of low fishmeal on the metabolic response of L. vannamei [25]. Thus, this technology empowers researchers to dissect the molecular mechanisms underlying the response of L. vannamei to endogenous and exogenous environmental factors.…”

Section: Introductionmentioning

confidence: 99%

Investigating the Impact of Varied Dietary Protein Levels on Litopenaeus vannamei: An Exploration of the Intestinal Microbiota and Transcriptome Responses

Li,

Yuan,

et al. 2024

Animals

Self Cite

View full text Add to dashboard Cite

This study explored the effects of dietary protein levels on Litopenaeus vannamei with its intestinal microbiota and transcriptome responses. Previous studies on the effects of dietary protein levels on L. vannamei have focused on growth performance, antioxidant indices, and digestive enzyme activity, but few studies have been conducted at the microbiological and molecular levels. In this study, five isolipid experimental diets with protein levels of 32% (P32), 36% (P36), 40% (P40), 44% (P44), and 48% (P48) were used in an L. vannamei (0.63 ± 0.02 g) feeding trial for 56 days. At the end of the feeding trial, the growth performance, immunity, intestinal health, and transcriptional responses of L. vannamei were determined. This study demonstrated that higher protein levels (P44) led to superior weight gain and growth rates for L. vannamei, with lower feed conversion ratios (FCR) observed in the P48 and P44 groups compared to the P32 and P36 groups (p ≤ 0.05). The P44 and P48 groups also showed a notably higher protein efficiency ratio (PER) compared to others (p ≤ 0.05), and there was no significant difference between them. Upon Vibrio parahaemolyticus infection, the P48 group exhibited a significantly lower survival rate (SR) within 48 h, while during 72 h of white spot syndrome virus (WSSV) infection, the P44 group had a notably higher survival rate than the P32 group (p ≤ 0.05). Digestive enzyme activity and antioxidant levels in L. vannamei initially increased and then decreased as protein levels increased, usually peaking in the P40 or P44 groups. Lower dietary protein levels significantly reduced the relative abundance of beneficial bacteria and increased the relative abundance of pathogenic bacteria in the intestines of L. vannamei. Transcriptome sequencing analysis revealed that most differentially expressed genes (DEGs) were up-regulated and then down-regulated as dietary protein levels increased. Furthermore, KEGG pathway enrichment analysis indicated that several immune and metabolic pathways, including metabolic pathways, glutathione metabolism, cytochrome P450, and lysosome and pancreatic secretion, were significantly enriched. In summary, the optimal feed protein level for L. vannamei shrimp was 40–44%. Inappropriate feed protein levels reduced antioxidant levels and digestive enzyme activity and promoted pathogen settlement, deceasing factors in various metabolic pathways that respond to microorganisms through transcriptional regulation. This could lead to stunted growth in L. vannamei and compromise their immune function.

show abstract

Schizochytrium limacinum altered antioxidant capacity and transcriptome profiles in Pacific white shrimp fed a low-fishmeal diet

Cited by 3 publications

References 31 publications

The Effect of Dietary Protein Hydrolysate from Black Soldier Fly Larvae and Schizochytrium on Palatability, Nutrient Metabolites and Health Status in Beagle Dogs

The Effect of Dietary Protein Hydrolysate from Black Soldier Fly Larvae and Schizochytrium on Palatability, Nutrient Metabolites and Health Status in Beagle Dogs

Marine thraustochytrid: exploration from taxonomic challenges to biotechnological applications

Investigating the Impact of Varied Dietary Protein Levels on Litopenaeus vannamei: An Exploration of the Intestinal Microbiota and Transcriptome Responses

Contact Info

Product

Resources

About