Scholarship Physics

Nelkon, M.; Armstrong, Hannah

doi:10.1119/1.1971034

Cited by 1 publication

(1 citation statement)

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“…However, since the bleb is part of a symmetric sphere, then the centers of curvatures are the same. Therefore, r 1 = r 2 = r, the single radius of curvature (Rand 1964; Nelkon 1979; Pellicer et al 2000). Thus…”

Section: Methodsmentioning

confidence: 99%

Biomechanics of the sarcolemma and costameres in single skeletal muscle fibers from normal and dystrophin-null mice

García‐Pelagio

Bloch

Ortega

et al. 2011

J Muscle Res Cell Motil

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We studied the biomechanical properties of the sarcolemma and its links through costameres to the contractile apparatus in single mammalian myofibers of Extensor digitorum longus muscles isolated from wild (WT) and dystrophin-null (mdx) mice. Suction pressures (P) applied through a pipette to the sarcolemma generated a bleb, the height of which increased with increasing P. Larger increases in P broke the connections between the sarcolemma and myofibrils and eventually caused the sarcolemma to burst. We used the values of P at which these changes occurred to estimate the tensions and stiffness of the system and its individual elements. Tensions of the whole system and the sarcolemma, as well as the maximal tension sustained by the costameres, were all significantly lower (1.8–3.3 fold) in muscles of mdx mice compared to WT. Values of P at which separation and bursting occurred, as well as the stiffness of the whole system and of the isolated sarcolemma, were ~2-fold lower in mdx than in WT. Our results indicate that the absence of dystrophin reduces muscle stiffness, increases sarcolemmal deformability, and compromises the mechanical stability of costameres and their connections to nearby myofibrils.

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Section: Methodsmentioning

confidence: 99%