Schwann cell interactions during the development of the peripheral nervous system

Wilson, Emma R.; Nunes, Gustavo Della-Flora; Weaver, Michael; Frick, Luciana Romina; Feltri, M. Laura

doi:10.1002/dneu.22744

Cited by 53 publications

(51 citation statements)

References 249 publications

(348 reference statements)

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“…In the direct coculture experiment, both cbMSCs and SCLCs exhibited a further increased potential in promoting neurite sprouting, especially the SCLC group. As the native SCs have close and complex interactions with axons (Wilson et al, 2020), it is not surprise that the direct interaction between SCLCs and SH-SY5Y cells could achieved the best result in stimulating neurite formation and elongation. Collectively, our results clearly demonstrated that cbMSCs can be phenotypically, epigenetically, FIGURE 3 | SCLCs were assembled into three-dimensional (3D) spheroids to enhance their therapeutic potential.…”

Section: Discussionmentioning

confidence: 99%

3D Spheroids of Umbilical Cord Blood MSC-Derived Schwann Cells Promote Peripheral Nerve Regeneration

Lin

Lee

Chang

et al. 2020

Front. Cell Dev. Biol.

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Schwann cells (SCs) are promising candidates for cell therapy due to their ability to promote peripheral nerve regeneration. However, SC-based therapies are hindered by the lack of a clinically renewable source of SCs. In this study, using a well-defined non-genetic approach, umbilical cord blood mesenchymal stem cells (cbMSCs), a clinically applicable cell type, were phenotypically, epigenetically, and functionally converted into SC-like cells (SCLCs) that stimulated effective sprouting of neuritic processes from neuronal cells. To further enhance their therapeutic capability, the cbMSC-derived SCLCs were assembled into three-dimensional (3D) cell spheroids by using a methylcellulose hydrogel system. The cell–cell and cell–extracellular matrix interactions were well-preserved within the formed 3D SCLC spheroids, and marked increases in neurotrophic, proangiogenic and anti-apoptotic factors were detected compared with cells that were harvested using conventional trypsin-based methods, demonstrating the superior advantage of SCLCs assembled into 3D spheroids. Transplantation of 3D SCLC spheroids into crush-injured rat sciatic nerves effectively promoted the recovery of motor function and enhanced nerve structure regeneration. In summary, by simply assembling cells into a 3D-spheroid conformation, the therapeutic potential of SCLCs derived from clinically available cbMSCs for promoting nerve regeneration was enhanced significantly. Thus, these cells hold great potential for translation to clinical applications for treating peripheral nerve injury.

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Section: Discussionmentioning

confidence: 99%

3D Spheroids of Umbilical Cord Blood MSC-Derived Schwann Cells Promote Peripheral Nerve Regeneration

Lin

Lee

Chang

et al. 2020

Front. Cell Dev. Biol.

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“…In the healthy PNS, Schwann cells myelinate peripheral nerves, increasing the conduction velocity of axonal impulses [ 36 ] (depicted in Figure 2 A). However, the abundant population of Schwann cells in the PNS serves many purposes outside of their role as the neuron-myelinating cell [ 37 ]. This includes enhancing and guiding axon regeneration, calcium signaling to modulate synaptic neurotransmission, and chemokine signaling to recruit nearby cells [ 38 , 39 ].…”

Section: Introductionmentioning

confidence: 99%

“…Electrospun fiber scaffolds are used to create in vitro culture systems that recapitulate the topographical features of axons in peripheral nerves and white matter tracts in the spinal cord, allowing for increased physiological relevance when studying interactions between glia and neurons in culture [ 37 , 45 , 48 ]. Additionally, electrospun fibers are readily incorporated into synthetic nerve grafts and assessed using in vivo animal models for their ability to support the infiltration of glia and axonal regeneration across the length of the injury [ 15 , 16 , 61 , 62 , 63 , 64 , 65 , 66 , 67 , 68 , 69 ].…”

Section: Introductionmentioning

confidence: 99%

Electrospun Fiber Scaffolds for Engineering Glial Cell Behavior to Promote Neural Regeneration

et al. 2020

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Electrospinning is a fabrication technique used to produce nano- or micro- diameter fibers to generate biocompatible, biodegradable scaffolds for tissue engineering applications. Electrospun fiber scaffolds are advantageous for neural regeneration because they mimic the structure of the nervous system extracellular matrix and provide contact guidance for regenerating axons. Glia are non-neuronal regulatory cells that maintain homeostasis in the healthy nervous system and regulate regeneration in the injured nervous system. Electrospun fiber scaffolds offer a wide range of characteristics, such as fiber alignment, diameter, surface nanotopography, and surface chemistry that can be engineered to achieve a desired glial cell response to injury. Further, electrospun fibers can be loaded with drugs, nucleic acids, or proteins to provide the local, sustained release of such therapeutics to alter glial cell phenotype to better support regeneration. This review provides the first comprehensive overview of how electrospun fiber alignment, diameter, surface nanotopography, surface functionalization, and therapeutic delivery affect Schwann cells in the peripheral nervous system and astrocytes, oligodendrocytes, and microglia in the central nervous system both in vitro and in vivo. The information presented can be used to design and optimize electrospun fiber scaffolds to target glial cell response to mitigate nervous system injury and improve regeneration.

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“…121,122 Detailed reviews of Schwann cell development can be found elsewhere. [123][124][125][126] Recent work from several laboratories has highlighted how inhibition of the Hippo pathway, with consequent activation of YAP/TAZ, is essential for multiple steps of normal Schwann cell development, including radial sorting, proliferation and subsequent myelination. 92,127,128 YAP/TAZ are expressed throughout the Schwann cell lineage, but TAZ expression is higher between P3 and P15, the period corresponding to active radial sorting and myelination.…”

Section: Dorsal Root Ganglia Neurons and Satellite Cellsmentioning

confidence: 99%

The Hippo pathway: Horizons for innovative treatments of peripheral nerve diseases

Feltri

Weaver

Belin

et al. 2021

J Peripheral Nervous Sys

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Initially identified in Drosophila, the Hippo signaling pathway regulates how cells respond to their environment by controlling proliferation, migration and differentiation. Many recent studies have focused on characterizing Hippo pathway function and regulation in mammalian cells. Here, we present a brief overview of the major components of the Hippo pathway, as well as their regulation and function. We comprehensively review the studies that have contributed to our understanding of the Hippo pathway in the function of the peripheral nervous system and in peripheral nerve diseases. Finally, we discuss innovative approaches that aim to modulate Hippo pathway components in diseases of the peripheral nervous system.

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Schwann cell interactions during the development of the peripheral nervous system

Abstract: This is the author manuscript accepted for publication and has undergone full peer review but has not been through the copyediting, typesetting, pagination and proofreading process, which may lead to differences between this version and the Version of Record. Please cite this article as

Cited by 53 publications

References 249 publications

3D Spheroids of Umbilical Cord Blood MSC-Derived Schwann Cells Promote Peripheral Nerve Regeneration

3D Spheroids of Umbilical Cord Blood MSC-Derived Schwann Cells Promote Peripheral Nerve Regeneration

Electrospun Fiber Scaffolds for Engineering Glial Cell Behavior to Promote Neural Regeneration

The Hippo pathway: Horizons for innovative treatments of peripheral nerve diseases

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