Science‐in‐brief: Proteomics and metabolomics in equine veterinary science

Anderson, James

doi:10.1111/evj.13550

Cited by 3 publications

(1 citation statement)

References 43 publications

(45 reference statements)

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“…Lipidomic techniques were developed in the early 2000s [ 5 ] and the number of related scientific reports in the field have sky-rocketed ever since [ 6 ]. To date, this subfield of metabolomics has not yet been widely explored in equine medicine [ 7 ].…”

Section: Introductionmentioning

confidence: 99%

Lipids in Equine Airway Inflammation: An Overview of Current Knowledge

Mönki,

Mykkänen

2024

Animals

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Mild–moderate and severe equine asthma (MEA and SEA) are prevalent inflammatory airway conditions affecting horses of numerous breeds and disciplines. Despite extensive research, detailed disease pathophysiology and the differences between MEA and SEA are still not completely understood. Bronchoalveolar lavage fluid cytology, broadly used in clinical practice and in equine asthma research, has limited means to represent the inflammatory status in the lower airways. Lipidomics is a field of science that can be utilized in investigating cellular mechanisms and cell-to-cell interactions. Studies in lipidomics have a broad variety of foci, of which fatty acid and lipid mediator profile analyses and global lipidomics have been implemented in veterinary medicine. As many crucial proinflammatory and proresolving mediators are lipids, lipidomic studies offer an interesting yet largely unexplored means to investigate inflammatory reactions in equine airways. The aim of this review article is to collect and summarize the findings of recent lipidomic studies on equine airway inflammation.

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Section: Introductionmentioning

confidence: 99%

Lipids in Equine Airway Inflammation: An Overview of Current Knowledge

Mönki,

Mykkänen

2024

Animals

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Lipid species profiling of bronchoalveolar lavage fluid cells of horses housed on two different bedding materials

Mönki,

Holopainen,

Ruhanen

et al. 2023

Sci Rep

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The lipidome of equine BALF cells has not been described. The objectives of this prospective repeated-measures study were to explore the BALF cells’ lipidome in horses and to identify lipids associated with progression or resolution of airway inflammation. BALF cells from 22 horses exposed to two bedding materials (Peat 1—Wood shavings [WS]—Peat 2) were studied by liquid chromatography-tandem mass spectrometry (LC–MS/MS). The effects of bedding on lipid class and species compositions were tested with rmANOVA. Correlations between lipids and cell counts were examined. The BALF cells’ lipidome showed bedding-related differences for molar percentage (mol%) of 60 species. Whole phosphatidylcholine (PC) class and its species PC 32:0 (main molecular species 16:0_16:0) had higher mol% after Peat 2 compared with WS. Phosphatidylinositol 38:4 (main molecular species 18:0_20:4) was higher after WS compared with both peat periods. BALF cell count correlated positively with mol% of the lipid classes phosphatidylserine, sphingomyelin, ceramide, hexosylceramide, and triacylglycerol but negatively with PC. BALF cell count correlated positively with phosphatidylinositol 38:4 mol%. In conclusion, equine BALF cells’ lipid profiles explored with MS-based lipidomics indicated subclinical inflammatory changes after WS. Inflammatory reactions in the cellular lipid species composition were detected although cytological responses indicating inflammation were weak.

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