Scintigraphic evaluation of functional hepatic mass in patients with advanced breast cancer

Virgolini, Irene; Kornek, Gabriela; Höbart, J; Li, S-R; Raolerer, M; Bergmann, Helmar; Scheithauer, Werner; Pantev, T.; Angelberger, P.; Sinzinger, H.

doi:10.1038/bjc.1993.384

Cited by 14 publications

(5 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Carriers have included asialoglycoproteins of natural origin, synthesized neoglycoproteins, and synthetic polymers modified to contain galactose residues (2)(3)(4)(5)(6). Another approach in the design of carriers binding the asialoglycoprotein receptor involves the synthesis of low molecular weight compounds containing multiple galactose residues (7,8).…”

Section: Introductionmentioning

confidence: 99%

“…Two additional groups of three rats each were anesthetized and prepared as above. The first group of animals was injected with 100 mg/kg body weight asialofetuin (type II, Sigma Chemical Co., St. Louis, MO), an asialoglycoprotein receptor ligand, 5 min prior to administration of 4 mg/kg of arabinogalactan-57Co-DTPA. The second group of rats was injected with 57Co-DTPA, 0.48 /¿mol of Na5DTPA/kg body weight (7 /¿Ci 57Co/kg), which is the amount of chelate injected with the 4 mg/kg body weight of arabinogalactan-57Co-DTPA.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Arabinogalactan for Hepatic Drug Delivery

Groman¹,

Enriquez²,

Jung³

et al. 1994

Bioconjugate Chem.

112

View full text Add to dashboard Cite

Arabinogalactan, a polysaccharide from the tree Larix occidentalis, has been purified and its biological and physical properties described. Intravenous injection of radiolabeled arabinogalactan (4 mg/kg) in rats resulted in 52.5% of the dose being present in the liver, while prior injection of asialofetuin (100 mg/kg) reduced hepatic radioactivity to 3.54%. Gel chromatography indicates arabinogalactan is a single species of 19 kDa, while light scattering gave a molecular weight of 40 kDa. Glycosyl linkage analysis of arabinogalactan is consistent with a highly branched structure comprising a backbone of 1,3-linked galactopyranose connected by 1,3-glycosidic linkages, comprised of 3,4,6-,3,6-, and 3,4- as well as 3-linked residues. In the carbon-13 NMR spectra, the major resonances of arabinogalactan are assigned to beta-galactopyranose, beta-arabinofuranose, and beta-arabinopyranose. Arabinogalactan produced no adverse reactions in single intravenous dose (mouse, 5000 mg/kg) and repeat dose toxicity studies (rats, 500 mg/kg/day, 90 days). When tritiated arabinogalactan was injected, radioactivity cleared from the liver with a half-life of 3.42 days. Arabinogalactan has properties that make it suitable as a carrier for delivering diagnostic or therapeutic agents to hepatocytes via the asialoglycoprotein receptor.

show abstract

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Arabinogalactan for Hepatic Drug Delivery

Groman¹,

Enriquez²,

Jung³

et al. 1994

Bioconjugate Chem.

112

View full text Add to dashboard Cite

show abstract

“…In vitro studies have revealed that a 1000‐fold higher number of specific receptors for 123 I‐Tyr‐(A14) insulin is expressed in HCC tissue than in normal liver tissue (24). Based on these studies, radiolabelled insulin has been proposed as a potential imaging agent for the noninvasive visualization of HCC (23–29). In patients with HCC, insulin uptake by tumour lesions is higher than uptake in the surrounding normal tissue (Fig.…”

Section: Differential Diagnostic Aspects Of Common Focal Liver Lesionsmentioning

confidence: 99%

Focal Liver Lesions: Receptor Scintigraphy for Differential Diagnosis

Kienast

Kurtaran

2003

Imaging Decisions

View full text Add to dashboard Cite

“…Non-invasive methods for functional liver assessment are of great interest for patient management in a diversity of clinical settings including liver surgery and transplantation (de Graaf et al 2010 ; Kaibori et al 2011 ; Hoekstra et al 2013 ) as well as diagnosis (Virgolini et al 1990 ) and treatment monitoring (Virgolini et al 1993 ) of cancer. Additionally, it has been revealed that the evaluation of remnant liver function can help to discriminate different stages of alcoholic liver cirrhosis (Virgolini et al 1991 ) and could be used to differentiate areas of steatosis, fibrosis and cholestasis (Bennink et al 2012 ).…”

Section: Introductionmentioning

confidence: 99%

[68Ga]Ga-NODAGA-TriGalactan, a low molecular weight tracer for the non-invasive imaging of the functional liver reserve

Zierke,

Rangger,

Samadikhah

et al. 2024

EJNMMI radiopharm. chem.

View full text Add to dashboard Cite

Background Determination of the functional liver mass is important in a variety of clinical settings including liver surgery and transplantation. [99mTc]Tc-diethylenetriamine-pentaacetic acid galactosyl human serum albumin (99mTc-GSA) is a radiotracer targeting the asialoglycoprotein receptor (ASGR) and is routinely used in Japan for this purpose. Here we describe the development and evaluation of [68Ga]Ga-NODAGA-TriGalactan a low molecular weight PET-tracer targeting this structure. Results For synthesis TRIS as branching unit and NODAGA as chelator for labelling with [68Ga]Ga are included. Three galactose moieties are conjugated via a click chemistry approach resulting in the desired labelling precursor.68Ga-labelling could be accomplished in high radiochemical yield and purity. [68Ga]Ga-NODAGA-TriGalactan is very hydrophilic and revealed high plasma stability and low plasma protein binding. Fluorescence imaging showed binding on ASGR-positive organoids and the IC50-value was in the nanomolar range. Most importantly, both biodistribution as well as animal imaging studies using normal mice demonstrated high liver uptake with rapid elimination from all other organs leading to even higher liver-to-background ratios as found for 99mTc-GSA. Conclusion [68Ga]Ga-NODAGA-TriGalactan shows high in vitro stability and selectively binds to the ASGR allowing imaging of the functional liver mass with high contrast. Thus, our first generation compound resulted already in an alternative to 99mTc-GSA for imaging the functional liver reserve and might allow the broader use of this imaging technique.

show abstract

Scintigraphic evaluation of functional hepatic mass in patients with advanced breast cancer

Cited by 14 publications

References 18 publications

Arabinogalactan for Hepatic Drug Delivery

Arabinogalactan for Hepatic Drug Delivery

Focal Liver Lesions: Receptor Scintigraphy for Differential Diagnosis

[68Ga]Ga-NODAGA-TriGalactan, a low molecular weight tracer for the non-invasive imaging of the functional liver reserve

Contact Info

Product

Resources

About