Scintigraphy with In-111-labeled monoclonal antitumor antibodies: kinetics, biodistribution, and tumor detection.

Abstract

The purpose of this study was to evaluate and compare the kinetics, biodistribution, and tumor-depicting properties of three intact indium-111-labeled murine monoclonal antibodies (MoAb) and to determine if use of In-111-labeled F(ab')2 fragments of one of them had advantages over its intact counterpart for immunoscintigraphy. Ten patients with prostate cancer were studied with an anti-prostatic acid phosphatase MoAb (PAY-276), with a resultant tumor detection rate of 15%. Twenty-eight patients with melanoma were studied with ZME-018, a MoAb that targets the KD-240 melanoma antigen. Forty-three percent of the known lesions were detected. Forty patients with carcinoembryonic antigen (CEA)-producing tumors were studied, 24 with intact ZCE-025, and anti-CEA MoAb, and 16 with its F(ab')2 fragment. With use of intact ZCE-025, 34% of known lesions were detected versus 83% with its F(ab')2 fragment. The distribution of each MoAb appears unique unto itself with regard to kinetics, normal tissue distribution, and response to MoAb mass.

Pharmacokinetic analysis of antibody localization in human colon cancer: Comparison with immunoscintigraphy

“…The antibody was provided by Hybritech, Inc. (San Diego) in the U.S.A., and the immunoscintigraphy of colorectal cancer with "'Inlabeled ZCE-025 has been exclusively evaluated in the U.S.A. [2][3][4] Few papers have, however, dealt with its pharmacokinetic characterizations. 5 It is important to know how injected antibody is metabolized in humans from the point of antibody-guided targeting studies.…”

A transition metal complex (Venus flytrap cluster) for radioimmunodetection and radioimmunotherapy.

“…With few exceptions, monoclonal antibody (mAb)-chelator conjugates have been prepared with bifunctional reagents that are members of the aminocarboxylate chelator family, with derivatives of diethylenetriaminepentaacetic acid (DTPA) and ethylenediaminetetraacetic acid (EDTA) being most popular (1)(2)(3)(4)(5). These conjugates form stable radiopharmaceuticals with 1"'In that have been used for radioimmunodiagnosis of human tumors (6)(7)(8), although there is some concern about their uptake by normal tissues and possible metal release in vivo. Conjugates of this type, however, form less stable radiopharmaceuticals with 90Y and 67Cu (9,10), which can be used for radioimmunotherapy.…”

Anti-Melanoma Monoclonal Antibody 225.28S Immunoscintigraphy in Metastatic Melanoma

“…The role of scintigraphy with various melanoma-specific antibodies in the management of melanoma patients was evaluated in several studies [2][3][4][5][6][7][8][9], Our findings and those of others [2,7] do not confirm earlier reports stating that immunoscintigraphy with radiolabeled F(ab')2 fragments was a reliable procedure, with sensitivities of 69.9-100% [3 -5 , 8]. De tection is site-and size-dependent, being most sensitive in the case of lymph node and bone metastases (96 and 95%, respectively, vs. only 20.5% for cutaneous and subcutane ous tumors) [71.…”