Scissor-based fluorescent detection of pepsin using lysozyme-stabilized Au nanoclusters

Abstract

Highly selective and sensitive detection of pepsin was developed through peptic hydrolysis of lysozyme-stabilized gold nanoclusters at pH 3.

Protein Binding-Induced Surfactant Aggregation Variation: A New Strategy of Developing Fluorescent Aqueous Sensor for Proteins

“…29,30 The fluorescent nanoparticles show competitive sensitivity to pepsin (DL of 0.256 μg mL −1 ); however, this method needs much longer incubation time (180 min) for protein detection. 9 Voltammetric immunosensor presents higher sensitivity toward o-egg (DL of 0.83 pg mL −1 ) 31 but not to pepsin. Therefore, the present supramolecular fluorescent sensing platform represents a fast and highly sensitive fluorescent sensor to these two proteins.…”

“…The detection limits (DLs) of the present sensor system to pepsin and o-egg are determined to be 4.8 nM (0.168 μg mL –1 ) and 18.9 nM (0.837 μg mL –1 ), respectively, according to the 3σ IUPAC criteria. Presently, there are several methods that have been reported to detect these two proteins, such as chromatographic methods, − imprinting hybrid thin films or imprinting polymers, , fluorescent nanoparticles, and voltammetric immunosensor . By comparison, chromatographic analysis exhibits lower sensitivity at milligram levels, − while sensitivity information is not available for imprinting methods. , The fluorescent nanoparticles show competitive sensitivity to pepsin (DL of 0.256 μg mL –1 ); however, this method needs much longer incubation time (180 min) for protein detection .…”

“…A variety of materials have been used to fabricate fluorescent sensors for proteins. For example, water-soluble conjugated polymers, , protein-printed polymers, quantum dots , or carbon dots, nanoparticles, , and graphene oxide have been used to prepare fluorescent sensors for proteins. Usually, the materials used determine the detection environments, fluorescence stability or intensity, and biocompatibility of the sensor; however, the strategy employed has more influence on the sensing behaviors of the sensor, such as sensitivity, selectivity, simplicity, etc.…”

Detection of Fe(iii) and bio-copper in human serum based on fluorescent AuAg nanoclusters

“…Noble metal clusters are an emerging class of uorescent nanomaterials, such as Au nanoclusters (NCs) and Ag NCs, 1,2 circumventing most of the drawbacks of common uorescent compounds, which have drawn wide attention in single-molecule optoelectronic nanodevices, biological labeling, optical sensing, novel catalysis, and surface-enhanced Raman spectroscopy (SERS). [3][4][5][6][7][8] Recognition and quantication of metal ions are considerably signicant due to the fact that these ions play important roles in various biological and environmental processes. 9,10 In the past few years, there have been numerous reports on the design of uorescent sensors for the detection of various metal ions, such as Hg 2+ , 11 Fe 3+ , 12 Cu 2+ and Cd 2+ , 13,14 and Pb 2+ , 15 because of their high sensitivity, specicity, and realtime monitoring with fast response time.…”

A selective fluorescence turn-on sensing system for evaluation of Cu2+ polluted water based on ultra-fast formation of fluorescent copper nanoclusters

“…Recently, due to their special molecular, uorescent and low toxic properties, the application of nanoclusters has attracted more attention. [24][25][26][27][28][29][30][31][32][33][34][35][36] Among the nanoclusters, CuNCs are the most cost effective, but their application is still limited because of the probes' natural instability. As a result, only a few examples of CuNC-products have been employed in sensing applications (see Table S1 †).…”