Sclareol production in the moss Physcomitrella patens and observations on growth and terpenoid biosynthesis

Pan, Xiaoguang; Han, Lei; Zhang, Yuhua; Chen, Dongfang; Simonsen, Henrik Toft

doi:10.1007/s11816-015-0353-8

Cited by 42 publications

(36 citation statements)

References 41 publications

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“…However, a similar study by the same research group recorded biomass of ~1 g CDW L -1 in 14 d for the moss cultivated under similar conditions (Pan et al, 2015 (Zhan et al, 2014). However, our analyses were conducted in 400 mL scale-up compared to shake flask cultivations of the moss.…”

Section: Sesquiterpenoid Productivity Comparisons To Other Phototrophmentioning

confidence: 87%

Efficient phototrophic production of a high-value sesquiterpenoid from the eukaryotic microalga Chlamydomonas reinhardtii

Lauersen

Baier

Wichmann

et al. 2016

Metabolic Engineering

122

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Efficient phototrophic production of a high-value sesquiterpenoid from the eukaryotic microalga Chlamydomonas reinhardtii, Metabolic Engineering, http://dx.doi.org/10.1016/j.ymben.2016 This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting galley proof before it is published in its final citable form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. olaf.kruse@uni-bielefeld.de AbstractThe heterologous expression of terpene synthases in microbial hosts has opened numerous possibilities for bioproduction of desirable metabolites. Photosynthetic microbial hosts present a sustainable alternative to traditional fermentative systems, using freely available (sun)light and carbon dioxide as inputs for bio-production. Here, we report the expression of a patchoulol synthase from Pogostemon cablin Benth in the model green microalga Chlamydomonas reinhardtii. The sesquiterpenoid patchoulol was produced from the alga and was used as a marker of sesquiterpenoid production capacity. A novel strategy for gene loading was employed and patchoulol was produced up to 922 ± 242 µg g -1 CDW in six days. We additionally investigated the effect of carbon source on sesquiterpenoid productivity from C. reinhardtii in scale-up batch cultivations. It was determined that up to 1.03 mg L -1 sesquiterpenoid products could be produced in completely photoautotrophic conditions and that the alga exhibited altered sesquiterpenoid production metabolism related to carbon source.

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Section: Sesquiterpenoid Productivity Comparisons To Other Phototrophmentioning

confidence: 87%

Efficient phototrophic production of a high-value sesquiterpenoid from the eukaryotic microalga Chlamydomonas reinhardtii

Lauersen

Baier

Wichmann

et al. 2016

Metabolic Engineering

122

231

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“…A second line, pBK3, was generated by disrupting PpCPS/KS via targeted gene replacement using the pBK3 vector first described in Pan et al (2015) and utilizing the method described in King et al (2016), which contains a p35S-aph4-CaMVter cassette flanked by 5 0 -and 3 0 -genomic sequence of PpCPS/KS on its 5 0 and 3 0 end, respectively. Therefore, genomic sequence of PpCPS/KS was replaced with p35S-aph4-CaMVter, which gives hygromycin resistance.…”

Section: New Phytologistmentioning

confidence: 99%

The decision to germinate is regulated by divergent molecular networks in spores and seeds

et al. 2016

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Summary Dispersal is a key step in land plant life cycles, usually via formation of spores or seeds. Regulation of spore‐ or seed‐germination allows control over the timing of transition from one generation to the next, enabling plant dispersal. A combination of environmental and genetic factors determines when seed germination occurs. Endogenous hormones mediate this decision in response to the environment. Less is known about how spore germination is controlled in earlier‐evolving nonseed plants.Here, we present an in‐depth analysis of the environmental and hormonal regulation of spore germination in the model bryophyte Physcomitrella patens (Aphanoregma patens).Our data suggest that the environmental signals regulating germination are conserved, but also that downstream hormone integration pathways mediating these responses in seeds were acquired after the evolution of the bryophyte lineage. Moreover, the role of abscisic acid and diterpenes (gibberellins) in germination assumed much greater importance as land plant evolution progressed.We conclude that the endogenous hormone signalling networks mediating germination in response to the environment may have evolved independently in spores and seeds. This paves the way for future research about how the mechanisms of plant dispersal on land evolved.

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“…[1][2][3] The main application of sclareol is for synthesizing high-end substitutes of ambergris in the perfume industry. 4 rather than modify the branched chain. Only few strains can transform sclareol to sclareol glycol or sclareolide.…”

Section: Introductionmentioning

confidence: 99%

Comparative proteomic analyses of Hyphozyma roseonigra ATCC 20624 in response to sclareol

et al. 2019

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b r a z i l i a n j o u r n a l o f m i c r o b i o l o g y 4 9 S (2 0 1 8) 160-165 h t t p : / / w w w . b j m i c r o b i o l . c o m . b r / Associate Editor: Solange I. Mussatto Keywords: Comparative proteomic Hyphozyma roseonigra Sclareol Sclareol glycol Ambroxide a b s t r a c t Sclareol is an important intermediate for ambroxide synthesis industries. Hyphozyma roseonigra ATCC 20624 was the only reported strain capable of degrading sclareol to the main product of sclareol glycol, which is the precursor of ambroxide. To date, knowledge is lacking about the effects of sclareol on cells and the proteins involved in sclareol metabolism. Comparative proteomic analyses were conducted on the strain H. roseonigra ATCC 20624 by using sclareol or glucose as the sole carbon source. A total of 79 up-regulated protein spots with a >2.0-fold difference in abundance on 2-D gels under sclareol stress conditions were collected for further identification. Seventy spots were successfully identified and finally integrated into 30 proteins. The up-regulated proteins under sclareol stress are involved in carbon metabolism; and nitrogen metabolism; and replication, transcription, and translation processes. Eighteen up-regulated spots were identified as aldehyde dehydrogenases, which indicating that aldehyde dehydrogenases might play an important role in sclareol metabolism. Overall, this study may lay the fundamentals for further cell engineering to improve sclareol glycol production.

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Sclareol production in the moss Physcomitrella patens and observations on growth and terpenoid biosynthesis

Cited by 42 publications

References 41 publications

Efficient phototrophic production of a high-value sesquiterpenoid from the eukaryotic microalga Chlamydomonas reinhardtii

Efficient phototrophic production of a high-value sesquiterpenoid from the eukaryotic microalga Chlamydomonas reinhardtii

The decision to germinate is regulated by divergent molecular networks in spores and seeds

Comparative proteomic analyses of Hyphozyma roseonigra ATCC 20624 in response to sclareol

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