Sclerotia of a phytopathogenic fungus restrict microbial diversity and improve soil health by suppressing other pathogens and enriching beneficial microorganisms

Mehmood, Mirza Abid; Zhao, Huizhang; Chéng, Jiāsēn; Xiè, Jiǎtāo; Fù, Yànpíng

doi:10.1016/j.jenvman.2019.109857

Cited by 17 publications

(10 citation statements)

References 140 publications

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“…Some species of the genus Schizothecium improved the growth of pumpkin under Verticillium dahliae stress 50 . Previous studies confirmed the antagonistic effect of Vermispora against Meloidogyne graminicola , Rhizoctonia solani , and nematodes 51 . Several species of the genus Podospora have antifungal functions 52 .…”

Section: Discussionsupporting

confidence: 59%

Synergistic effects of crop residue and microbial inoculant on soil properties and soil disease resistance in a Chinese Mollisol

Liu

Wang

et al. 2021

Sci Rep

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The soil-borne disease caused by Fusarium graminearum seriously affects the corn quality. Straw can greatly improve soil quality, but the effect is limited by its nature and environmental factors. This study explored the impact of straw-JF-1(biocontrol bacteria) combination on soil environment and soil disease resistance. The results showed that the combined treatment increased the proportion of soil large and small macro-aggregates by 22.50 and 3.84%, with soil organic carbon (SOC) content by 16.18 and 16.95%, respectively. Compared to treatment with returning straw to the field alone, the straw-JF-1 combination increased the soil content of humic acid, fulvic acid, and humin by 14.06, 5.50, and 4.37%, respectively. Moreover, A metagenomics showed that returning straw to the field alone increased the abundance of disease-causing fungi (Fusarium and Plectosphaerella), however, the straw-JF-1 combination significantly suppressed this phenomenon as well as improved the abundance of probiotic microorganisms such as Sphingomonas, Mortierella, Bacillus, and Pseudomonas. Functional analysis indicated that the combination of straw and JF-1 improved some bacterial functions, including inorganic ion transport and metabolism, post-translational modification/protein turnover/chaperones and function unknown, fungal functions associated with plant and animal pathogens were effectively inhibited. Pot experiments showed that the straw-JF-1 combination effectively inhibited the Fusarium graminearum induced damage to maize seedlings. Therefore, the combination of straw and JF-1 could be a practical method for soil management.

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Section: Discussionsupporting

confidence: 59%

Synergistic effects of crop residue and microbial inoculant on soil properties and soil disease resistance in a Chinese Mollisol

Liu

Wang

et al. 2021

Sci Rep

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“…In our previous study, we found that several bacterial genera, notably, Achromobacter, Burkholderia, Chitinophaga, Dyella, Kribbella, Sphingomonas, Mesorhizobium, and Rhizobium with a known potential role as biocontrol agents, plant growth promoters, and biological nitrogen fixation depicted increased abundance in Sclerotinia sclerotiorum-amended samples (Mehmood et al, 2020). In this study, we observed that Kribbella with a reported role as a biocontrol agent (Igarashi et al, 2017), Chitinophaga (Esitken et al, 2005;Yin et al, 2013), Flavisolibacter (Xiao et al, 2017), and Dyella (Anandham et al, 2008;Palaniappan et al, 2010) with the reported role of plant growth promotion depicted enhanced abundance in R. solani sclerotia-amended soil samples.…”

Section: Discussionmentioning

confidence: 90%

“…Equal quantities of the soil samples were thoroughly mixed to get a composite sample. Soil samples were dried at room temperature subsequently, small roots were removed with the help of forceps, and samples were passed through a 2 mm mesh size sieve as explained by others (Steinbeiss et al, 2008;Mehmood et al, 2020).…”

Section: Soil Samples Preparation and Amendment Of Sclerotiamentioning

confidence: 99%

“…All the non-amended and amended samples were incubated at 28 ± 2 °C with 12 h/12 h day/night photoperiod in a growth chamber for three months. During the entire experimental period, the water-filled pore space (WFPS) of the soil samples were maintained to 60-80% by weighing the pots and adding sterilized distilled water (Lin et al, 2013;Mehmood et al, 2020). Every month, approximately 10 g of soil from each sample was collected and preserved at -80 °C.…”

Section: Soil Samples Preparation and Amendment Of Sclerotiamentioning

confidence: 99%

“…Quantification of the extracted DNA concentration was checked using a Qubit 2.0 Fluorometer (Invitrogen, Carlsbad, CA, USA). Moreover, V3 and V4 hypervariable regions of bacterial 16S ribosomal RNA were targeted using forward and reverse primer with sequences "CCT ACG GRRBGCASCAGKVRVGAAT" and "GGA CTA CNVGGG TWT CTA ATC C" specially designed by GENEWIZ (GENEWIZ Inc., South Plainfield, NJ, USA) (Caporaso et al, 2011;Yang et al, 2016;Mehmood et al, 2020). Furthermore, to get the uniform amplification of libraries, 16S rRNA primers were supplemented with indexed adapter sequences.…”

Section: Extraction Of Soil Dna Amplification and Sequencingmentioning

confidence: 99%

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Enrichment of bacteria involved in the nitrogen cycle and plant growth promotion in soil by sclerotia of rice sheath blight fungus

et al. 2022

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Rice sheath blight pathogen, Rhizoctonia solani, produces numerous sclerotia to overwinter. As a rich source of nutrients in the soil, sclerotia may lead to the change of soil microbiota. For this purpose, we amended the sclerotia of R. solani in soil and analyzed the changes in bacterial microbiota within the soil at different time points. At the phyla level, Proteobacteria, Acidobacteria, Bacteroidetes, Actinobacteria, Chloroflexi and Firmicutes showed varied abundance in the amended soil samples compared to those in the control. An increased abundance of ammonia-oxidizing bacterium (AOB) Nitrosospira and Nitrite oxidizing bacteria (NOB) i.e., Nitrospira was observed, where the latter is reportedly involved in the nitrifier denitrification. Moreover, Thiobacillus, Gemmatimonas, Anaeromyxobacter and Geobacter, the vital players in denitrification, N2O reduction and reductive nitrogen transformation, respectively, depicted enhanced abundance in R. solani sclerotia-amended samples. Furthermore, asymbiotic nitrogen-fixing bacteria, notably, Azotobacter as well as Microvirga and Phenylobacterium with nitrogen-fixing potential also enriched in the amended samples compared to the control. Plant growth promoting bacteria, such as Kribbella, Chitinophaga and Flavisolibacter also enriched in the sclerotia-amended soil. As per our knowledge, this study is of its kind where pathogenic fungal sclerotia activated microbes with a potential role in N transformation and provided clues about the ecological functions of R. solani sclerotia on the stimulation of bacterial genera involved in different processes of N-cycle within the soil in the absence of host plants.

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Nano‐Bio Interactions and Their Practical Implications in Agriculture

Bezbaruah

Fortuna

2021

Microbial Interactions at Nanobiotechnology Interfaces

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Sclerotia of a phytopathogenic fungus restrict microbial diversity and improve soil health by suppressing other pathogens and enriching beneficial microorganisms

Cited by 17 publications

References 140 publications

Synergistic effects of crop residue and microbial inoculant on soil properties and soil disease resistance in a Chinese Mollisol

Synergistic effects of crop residue and microbial inoculant on soil properties and soil disease resistance in a Chinese Mollisol

Enrichment of bacteria involved in the nitrogen cycle and plant growth promotion in soil by sclerotia of rice sheath blight fungus

Nano‐Bio Interactions and Their Practical Implications in Agriculture

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