Sclerotinia sclerotiorum catalase SCAT1 affects oxidative stress tolerance, regulates ergosterol levels and controls pathogenic development

Yarden, Oded; Veluchamy, Selvakumar; Dickman, Martin B.; Kabbage, Mehdi

doi:10.1016/j.pmpp.2013.12.001

Cited by 39 publications

(32 citation statements)

References 61 publications

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“…For example, in agreement with the role of SOD for B. cinerea infection, S. sclerotiorum also requires SOD for pathogenicity due to its role in oxalate production, a secreted metabolite required for pathogenicity (Veluchamy et al, 2012). However, in contrast to the role of CatB in the necrotrophs B. cinerea and C. heterostophus, catalases were required for pathogenicity by S. sclerotiorum but not for scavenging extracellular host-derived ROS, which is similar to the role of CatB described for M. oryzae (Robbertse et al, 2003;Schouten et al, 2002;Yarden et al, 2014).…”

Section: Necrotrophssupporting

confidence: 64%

Reactive oxygen species metabolism and plant-fungal interactions

Segal

Wilson

2018

Fungal Genetics and Biology

165

103

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Fungal interactions with plants can involve specific morphogenetic developments to access host cells, the suppression of plant defenses, and the establishment of a feeding lifestyle that nourishes the colonizer oftenbut not always-at the expense of the host. Reactive oxygen species (ROS) metabolism is central to the infection process, and the stage-specific production and/or neutralization of ROS is critical to the success of the colonization process. ROS metabolism during infection is dynamicsometimes seemingly contradictory-and involves endogenous and exogenous sources. Yet, intriguingly, molecular decision-making involved in the spatio-temporal control of ROS metabolism is largely unknown. When also considering that ROS demands are similar between pathogenic and beneficial fungal-plant interactions despite the different outcomes, the intention of our review is to synthesize what is known about ROS metabolism and highlight knowledge gaps that could be hindering the discovery of novel means to mediate beneficial plant-microbe interactions at the expense of harmful plant-microbe interactions.

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Section: Necrotrophssupporting

confidence: 64%

Reactive oxygen species metabolism and plant-fungal interactions

Segal

Wilson

2018

Fungal Genetics and Biology

165

103

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“…In addition to the well-known effectors that have been mentioned above, a number of genes encoding other S. sclerotiorum effectors, including SsBi1 [20], SsCaf1 [25], SCat1 [18], SsSodI [19] and SsPemG1 [29] were expressed, but not significantly induced in the current study. The discrepancy might be due to host and isolate-dependent differences in the expression of these genes or differences in experimental design.…”

Section: Discussionmentioning

confidence: 49%

“…Pathogen-derived ROS generated through NADPH oxidase activity are associated with appressoria formation and sclerotial development, as well as oxalic acid synthesis [8]. Catalase ( SCat1 ) [18] and superoxide dismutase ( SsSodI ) [19] appear to modulate the deleterious effects of these compounds internally. The apoptosis inhibitor, BAX inhibitor-1 ( SsBI1 ), is also required for full virulence and was postulated to prevent hyphal apoptosis resulting from exposure to host-derived ROS [20].…”

Section: Introductionmentioning

confidence: 99%

Changes in the Sclerotinia sclerotiorum transcriptome during infection of Brassica napus

et al. 2017

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Background Sclerotinia sclerotiorum causes stem rot in Brassica napus, which leads to lodging and severe yield losses. Although recent studies have explored significant progress in the characterization of individual S. sclerotiorum pathogenicity factors, a gap exists in profiling gene expression throughout the course of S. sclerotiorum infection on a host plant. In this study, RNA-Seq analysis was performed with focus on the events occurring through the early (1 h) to the middle (48 h) stages of infection.ResultsTranscript analysis revealed the temporal pattern and amplitude of the deployment of genes associated with aspects of pathogenicity or virulence during the course of S. sclerotiorum infection on Brassica napus. These genes were categorized into eight functional groups: hydrolytic enzymes, secondary metabolites, detoxification, signaling, development, secreted effectors, oxalic acid and reactive oxygen species production. The induction patterns of nearly all of these genes agreed with their predicted functions. Principal component analysis delineated gene expression patterns that signified transitions between pathogenic phases, namely host penetration, ramification and necrotic stages, and provided evidence for the occurrence of a brief biotrophic phase soon after host penetration.ConclusionsThe current observations support the notion that S. sclerotiorum deploys an array of factors and complex strategies to facilitate host colonization and mitigate host defenses. This investigation provides a broad overview of the sequential expression of virulence/pathogenicity-associated genes during infection of B. napus by S. sclerotiorum and provides information for further characterization of genes involved in the S. sclerotiorum-host plant interactions.Electronic supplementary materialThe online version of this article (doi:10.1186/s12864-017-3642-5) contains supplementary material, which is available to authorized users.

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“…Partial rescue of the ΔBbThm1 phenotype by supplementation with ergosterol Ergosterol biosynthesis has been linked to membrane integrity in a number of fungi (Dupont et al, 2011;Yarden et al, 2014;. In order to examine whether loss of BbThm1 could be linked to limitations in membrane ergosterol levels, phenotypic analyses were performed in media supplemented with 2.5 mM ergosterol ( Fig.…”

Section: Resultsmentioning

confidence: 99%

“…Ergosterol content was measured as described (Arthington-Skaggs et al, 1999;Yarden et al, 2014). Briefly, 1 ml of conidial suspensions (10 7 conidia/ml) were inoculated in 100 ml of SDB and incubated at room temperature for 3 days with aeration.…”

Section: Ergosterol Measurementsmentioning

confidence: 99%

The Thm1 Zn(II)₂Cys₆ transcription factor contributes to heat, membrane integrity and virulence in the insect pathogenic fungus Beauveria bassiana

Huang

Keyhani

Zhao

et al. 2019

Environmental Microbiology

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Summary The ability to withstand heat and cell wall stress is critical for successful infection of target hosts by many pathogenic fungi. We report on the characterization of BbThm1 (transcription factor for heat and membrane integrity), a Zn(II)2Cys6 (Gal4‐like) family member, which regulates resistance to heat (32°C) and specific detergents in the insect pathogenic fungus, Beauveria bassiana. BbThm1 gene knock‐out mutants showed severely impaired growth/resistance at 32°C and to SDS, but mild to moderate phenotypic responses to other stresses including oxidative, osmotic and cell‐wall targeting compounds, or to various fungicides. Shifts in cell wall properties, adhesion and decreased viability were noted for the ΔBbThm1 mutant. Susceptibility to SDS but not heat could be rescued by exogenous ergosterol. Insect bioassays revealed decreased virulence of the ΔBbThm1 mutant against Galleria mellonella both topically and via intrahemocoel injection assays that correlated with impaired in insecta hyphal body formation and altered host immune prophenol oxidase (PO) activation. Transcriptomic analyses of the wild‐type and ΔBbThm1 mutants revealed up‐regulation of hydrolases but down‐regulation of a wide range of basic metabolic processes. These data reveal fine tune aspects of the transcription factor network that regulates specific stress responses to contribute to the overall pathogenic process.

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Sclerotinia sclerotiorum catalase SCAT1 affects oxidative stress tolerance, regulates ergosterol levels and controls pathogenic development

Cited by 39 publications

References 61 publications

Reactive oxygen species metabolism and plant-fungal interactions

Reactive oxygen species metabolism and plant-fungal interactions

Changes in the Sclerotinia sclerotiorum transcriptome during infection of Brassica napus

The Thm1 Zn(II)₂Cys₆ transcription factor contributes to heat, membrane integrity and virulence in the insect pathogenic fungus Beauveria bassiana

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Sclerotinia sclerotiorum catalase SCAT1 affects oxidative stress tolerance, regulates ergosterol levels and controls pathogenic development

Cited by 39 publications

References 61 publications

Reactive oxygen species metabolism and plant-fungal interactions

Reactive oxygen species metabolism and plant-fungal interactions

Changes in the Sclerotinia sclerotiorum transcriptome during infection of Brassica napus

The Thm1 Zn(II)2Cys6 transcription factor contributes to heat, membrane integrity and virulence in the insect pathogenic fungus Beauveria bassiana

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The Thm1 Zn(II)₂Cys₆ transcription factor contributes to heat, membrane integrity and virulence in the insect pathogenic fungus Beauveria bassiana