Scope and limitations of a multiplex conventional PCR for the diagnosis of S. stercoralis and hookworms

Fleitas, Pedro Emanuel; Vargas, Paola A.; Caro, Nicolás; Almazán, María Cristina; Echazú, Adriana; Juárez, Marisa; Cajal, Pamela; Krolewiecki, Alejandro; Nasser, Julio R.; Cimino, Rubén O.

doi:10.1016/j.bjid.2021.101649

Cited by 4 publications

(2 citation statements)

References 29 publications

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“…Polymerase chain reaction (PCR)-based methods possess high sensitivity as parasite detection is possible with low DNA concentrations ( Manuel et al ., 2021 ; Verweij et al ., 2001 ). A recent study by Fleitas et al ., (2021) reported successful detection of S. stercoralis and hookworm in stool samples with 2 – 20 pg genomic DNA. The sensitivity for S. stercoralis and hookworms was 97.4 % and 90.3 %, while the specificity was 100 % and 87.6 %, respectively ( Fleitas et al ., 2021 ).…”

Section: Conventional Pcrmentioning

confidence: 93%

“…A recent study by Fleitas et al ., (2021) reported successful detection of S. stercoralis and hookworm in stool samples with 2 – 20 pg genomic DNA. The sensitivity for S. stercoralis and hookworms was 97.4 % and 90.3 %, while the specificity was 100 % and 87.6 %, respectively ( Fleitas et al ., 2021 ). Conventional PCR is also used with other downstream techniques, such as restriction fragment length polymorphism (RFLP), to identify different species of STH ( George et al ., 2016 ; Manuel et al ., 2021 ).…”

Section: Conventional Pcrmentioning

confidence: 93%

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Advantages and limitations of microscopy and molecular detections for diagnosis of soil-transmitted helminths: An overview

2022

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Summary World Health Organization (WHO) reported that over 1.5 billion people are infected by soil-transmitted helminths (STH) worldwide in sub-Saharan Africa, the United States of America, China, and East Asia. Heavy infections and polyparasitism are associated with higher morbidity rates, and the patients are exposed to increased vulnerability to other diseases. Therefore, accurate diagnosis followed by mass treatment for morbidity control is necessary.STH diagnosis commonly involves the microscopic observation of the presence of the STH eggs and larvae in the faecal samples. Furthermore, molecular approaches are increasingly utilised in monitoring and surveillance as they show higher sensitivity. Their capability to differentiate hookworm species is an advantage over the Kato-Katz technique. This review discusses the advantages and limitations of microscopy and various molecular tools used for STH detection.

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