Scope of Genetic Transformation in Sugarcane-A Review

Embryogenic Competence Acquisition in Sugar Cane Callus Is Associated with Differential H+-Pump Abundance and Activity

“…Genetic transformation has been successfully used for several purposes, such as to promote resistance to herbivory, drought, and herbicides and to increase sugar content; however, low transformation rates and inefficient regeneration protocols prevent the high-throughput transformation of sugar cane . Optimization of the regeneration protocol is dependent on advances in in vitro culture techniques, and somatic embryogenesis demonstrates great potential as a regeneration technique for transformed plants . Therefore, somatic embryogenesis is more effective for the regeneration of plants derived from both particle bombardment and Agrobacterium -mediated transformation techniques …”

“…9 Optimization of the regeneration protocol is dependent on advances in in vitro culture techniques, and somatic embryogenesis demonstrates great potential as a regeneration technique for transformed plants. 10 Therefore, somatic embryogenesis is more effective for the regeneration of plants derived from both particle bombardment and Agrobacteriummediated transformation techniques. 7 In general, somatic embryogenesis protocols include induction, multiplication, maturation, and conversion phases, 11 and recent studies have sought to elucidate the main molecular mechanisms associated with the process of somatic embryo formation in different species.…”

Introductory Chapter: Bioengineered Sugarcane - A Sustainable Biofactory of Renewable Energy

“…How the process of 'dead heart' starts, the young larvae tunnels into the nucleus of the spindle and damage the growing point, causing the shoot to wilt and die. This requires the development of resistance against several factors including biotic and abiotic in sugarcane [9], but traditional improvement strategies are hampered by high polyploidy, genetic complexity, environment-specificity, and low fertility [10]. Thus biotechnological approaches have the potential to address such stresses by engineering resistance.…”